Tomáš Lošák

Intercropping of arable crops might provide yield benefits over monocrops. To assess the effect of sowing date and sowing ratio on biomass formation and competition over time, a two-year field experiment with wheat and pea plants was performed in Eastern Austria with two sowing times (autumn vs. spring) and with the following substitutive wheat–pea sowing ratios (%:%): 100:0, 75:25, 50:50, 25:75, 12.5:87.5 and 0:100. For both crops, facultative cultivars were used. Compared to spring-sowing, autumn-sowing resulted in earlier development of plants, taller plants, faster soil coverage, a higher crop growth rate up to mid-May in the first year and early June in the second year, more above-ground dry matter production and in the second year also in a higher land equivalent ratio (LER) of intercrops. Sowing ratios affected absolute and relative growth rates of wheat and pea plants. Wheat, which was generally the stronger partner in the intercrops, out-competed pea plants in all intercrops in the first year due to a higher early crop growth rate and in the second year, when the monocrop biomass of wheat was lower than that of pea plants, even in the intercrops with lower wheat and higher pea share. All intercrops resulted in a yield advantage compared to the monocrops as indicated by the LER. At final harvest, this yield advantage was over both sowing times and all four intercropping ratios 14% in the first and 10% in the second year. The competitive abilities of individual crops in mixtures, as indicated by the partial LER, were not affected by the sowing time.

The labile fractions of?organic carbon (OC), which are a?reflection of?the properties of?soil and its use, appear to?be suitable parameters for their use as?indicators. The aim of?this study was to?determine the reliable and relatively simple ...

The invasion of plant species is considered to be one of the most dangerous forces in biodiversity change and alteration of soil properties. Due to their significant impact on ecology and the economy, it is important to find an effective approach to manage invasive plant expansion and utilize them as a beneficial biomass source. This review focuses on the characterization of the negative and positive features of invasive plant species in general. Most studies focus on invasive species removal and lack an evaluation of their potential in modern biotechnologies. Currently, there are studies aimed at investigating their use in soil remediation, medicine, the chemical industry, the textile industry, and even gastronomy. Based on these reviews, we bring forward possible future developments in this research field which might serve as a theoretical premise for further research.

The content of this chapter symbolically closes the process of biomass production and use. Referring to the circulation of matter in nature, its aim is to show the full potential of biomass at a stage which, in conventional management, would mean waste. In the context of the circular bioeconomy, by contrast, it is something of value, necessary for further biomass production. The chapter therefore starts with a presentation of the concept of the natural recycling of biomatter (a form of ‘waste’) as nutrients and how those nutrients are turned into fertilisers. This naturally occurring or controlled process is placed within the legal and institutional framework, relating, inter alia, to the definition and status of ‘waste’. These conditions differ between individual EU member states, a subject which is also addressed in this chapter. The use of biowaste for further biomass production or remediation has significant economic, social and environmental impacts, which are also discussed. In the final part of the chapter, the authors present the results of their own experiments in the form of case studies of mixtures of sewage sludge and cellulose waste, and of biomass ash with sewage sludge, as soil improvers.

Due to the typical production of Cannabis sativa L. for medical use in an artificial environment, it is crucial to optimize environmental and nutritional factors to enhance cannabinoid yield and quality. While the effects of light intensity and nutrient composition on plant growth are well-documented for various crops, there is a relative lack of research specific to Cannabis sativa L., especially in controlled indoor environments where both light and nutrient inputs can be precisely manipulated. This research analyzes the effect of different light intensities and nutrient solutions on growth, flower yield, and cannabinoid concentrations in seeded chemotype III cannabis (high CBD, low THC) in a controlled environment. The experiment was performed in a licensed production facility in the Czech Republic. The plants were exposed to different light regimes during vegetative phase and flowering phase (light 1 (S1), photosynthetic photon flux density (PPFD) 300 µmol/m²/s during vegetative phase, 900 µmol/m²/s in flowering phase and light 2 (S2) PPFD 500 µmol/m²/s during vegetative phase, 1300 µmol/m²/s during flowering phase) and different nutrition regimes R1 (fertilizer 1) and R2 (fertilizer 2). Solution R1 (N-NO₃⁻ 131.25 mg/L; N-NH₄⁺ 6.23 mg/L; P₂O₅ 30.87 mg/L; K₂O 4112.04 mg/L; CaO 147.99 mg/L; MgO 45.68 mg/L; SO₄²⁻ 45.08 mg/L) was used for the whole cultivation cycle (vegetation and flowering). Solution R2 was divided for vegetation phase (N-NO₃⁻ 171.26 mg/L; N-NH₄⁺ 5.26 mg/L; P₂O₅ 65.91 mg/L; K₂O 222.79 mg/L; CaO 125.70 mg/L; MgO 78.88 mf/L; SO₄²⁻ 66.94 mg/L) and for flowering phase (N-NO₃⁻ 97.96 mg/L; N-NH₄⁺ 5.82 mg/L; P₂O₅ 262.66 mg/L; K₂O 244.07 mg/L; CaO 138.26 mg/L; MgO 85.21 mg/L; SO₄²⁻ 281.54 mg/L). The aim of this study was to prove a hypothesis that light will have a significant impact on the yield of flowers and cannabinoids, whereas fertilizers would have no significant effect. The experiment involved a four-week vegetative phase followed by an eight-week flowering phase. During the vegetative and flowering phases, no nutrient deficiencies were observed in plants treated with either nutrient solution R1 (fertilizer 1) or R2 (fertilizer 2). The ANOVA analysis showed that fertilizers had no significant effect on the yield of flowers nor cannabinoids. Also, light intensity differences between groups S1 (light 1) and S2 (light 2) did not result in visible differences in plant growth during the vegetative stage. However, by the fifth week of the flowering phase, plants under higher light intensities (S2—PPFD 1300 µmol/m²/s) developed noticeably larger and denser flowers than plants in the lower light intensity group (S1). The ANOVA analysis also confirmed that the higher light intensities positively influenced cannabidiol (CBD), tetrahydrocannabinol (THC), cannabigerol (CBG), and cannabichromene (CBC) when the increase in the concentration of individual cannabinoids in the harvested product was 17–43%. Nonetheless, the study did not find significant differences during the vegetative stage, highlighting that the impact of light intensities is phase-specific. These results are limited to controlled indoor conditions, and further research is needed to explore their applicability to other environments and genotypes.

Invasion of plant species has been considered as one of the most dangerous force in biodiversity changes and alteration of soil properties. Due to their significant impacts on ecology and the economy, it is important to find an effective approaches to manage the invasive plants expansion and utilize them as beneficial biomass sources. This review focuses on the characterization of the negative and positive features of invasive plant species in general. Most studies targeted on invasive species removal and lacked an evaluation of their potentials in modern biotechnologies. Currently, there are studies aimed at their use in soil remediation, medicine, the chemical industry, the textile industry, and even gastronomy. Based on these reviews, we bring forward possible future development in this research field, which might serve as a theoretical premise for further researches.

Chickpea (Cicer arietinum L.) is one of the most important legumes currently grown. It is an important source of proteins and nutrients, such as calcium, potassium and iron. As a result, precise crop management is necessary for maximizing its production. The presented study deals with the effect of soil heterogeneity caused by variable contents of macro- and micronutrients on the uptake of nutrients by chickpea. The values measured (contents of macro- and micronutrients in plant samples) indicate that soil heterogeneity is an important factor for the contents of nutrients and soil reactions, which strongly affect the growth of chickpea. We investigated the soil heterogeneity in a chickpea field. Two zones (A and B) with different stand development were found in the model plot. Zone A showed a healthy (green) growth, while Zone B exhibited a yellow-coloured growth, indicating deficits in nutrient uptake. The contents of selected nutrients (P, K, Ca, Mg, Fe, Cu, Zn and Mn) in the soil and in the plant biomass (i.e., stems, leaves, pods and seeds) were analyzed. In the zone with the yellow-coloured biomass, the results showed significantly (p < 0.05) reduced contents of N, P, K, Mg, Fe, Mn, Cu and Zn in the leaves; higher values of soil reaction (pH); and higher contents of calcium and calcium carbonate in the soil. The uptake of nutrients by the plants and their translocation were affected by the above-mentioned soil parameters and by their mutual interactions. Therefore, it is possible to state that soil heterogeneity (caused by variable contents of nutrients in soil) should be taken into account in the precise crop management of chickpeas.

The demand for agricultural products is growing and is resulting in significant environmental impacts due to the overuse of fertilizers (and pesticides in some cases). There is a continued need to find sustainable methods in agricultural systems without harming the environment. Regenerative agriculture can be considered as one of the best methods of sustainable agriculture. The aim of this comparative life cycle assessment (LCA) study was to quantify the environmental impacts associated with the production of silage maize at different doses of fertilizers and pesticides under conventional agriculture and without the use of fertilizers and pesticides under regenerative agriculture. The input data were obtained from the experimental fields and supplemented by background process databases of Ecoinvent, World Food Live Cycle Assessment Database (WFLCD), and the French database AGRIBALYSE. The results of the study were related to six midpoint impact categories: global warming, marine eutrophication, freshwater eutrophication, freshwater ecotoxicity, marine ecotoxicity, and terrestrial ecotoxicity. Although the variant of growing silage maize without the use of fertilizers and pesticides according to the principle of regenerative agriculture showed the lowest burden on the environment, the yields of the cultivated silage maize were 43–55% lower than those of the fertilized variants.

Freezing soil samples at subzero temperatures is a commonly employed preservation method in soil science to halt microbial activity and enzymatic processes, preserving the sample’s composition and structure. The concentration of adenosine triphosphate (ATP) and the soil microbial biomass carbon content were determined from soil samples stored at +4 °C, −21 °C and −80 °C for 24 h, 7 days, and 20 days. The results showed that the ATP in the soil was not significantly affected by temperature or storage time. Larger differences were observed in the carbon content of microbial biomass, where the amount of this parameter increased by 14.2% after 20 days of the experiment.

Poppy cultivation has a long tradition in Central Europe. Growing winter poppy instead of the commonly grown spring poppy might increase seed yield, especially in the face of changing climatic conditions. However, knowledge regarding optimum sowing date and optimum sowing rate for winter poppy under Pannonian climate conditions in Central Europe is missing. Therefore, a two-year field experiment was performed in Eastern Austria with four sowing dates ranging from early September to mid/end of October and two sowing rates with 50 or 100 mg seeds m⁻². Seed yields were considerably higher than values reported for spring poppy throughout all sowing dates, mainly due to a higher number of seeds capsule⁻¹ and, thereby, a higher seed yield capsule⁻¹. The highest seed yields were obtained by sowing in early October, while the earliest and especially the latest sowing date resulted in lower seed yields. Consequently, the optimum sowing date for winter poppy under Pannonian climate conditions in Central Europe is early October but sowing can be performed over a wider range of dates. No seed yield differences were observed between sowing rates. Consequently, the sowing rate can be much lower than the recommended sowing rate for spring poppy.