Francesca Torrini
Research Subjects
Research Interests
Francesca Torrini has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
Alvaro Brittoli
Dipartimento di Chimica "Ugo Schiff", Via della Lastruccia 3-13, 50019, Sesto Fiorentino, FI, Italy
1 shared publicationMaria Minunni
Dipartimento di Chimica "Ugo Schiff", Via della Lastruccia 3-13, 50019, Sesto Fiorentino, FI, Italy
1 shared publication- Pasquale Palladino
University of Florence via della Lastruccia, 3-13
1 shared publication - Simona Scarano
University of Florence via della Lastruccia, 3-13
1 shared publication - Veronica Baldoneschi
Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3-13, 50019, Sesto Fiorentino, Italy
1 shared publication
Latest Publications
- 13 July 2019
- Published by Springer Nature in Analytical and Bioanalytical Chemistry
- Vol. 411 (29) , 7709-7716
Characterization of troponin T binding aptamers for an innovative enzyme-linked oligonucleotide assay (ELONA)
Early diagnosis of acute myocardial infarction (AMI) is of outmost importance to reduce the mortality rate, and cardiac troponins are considered the gold standard biomarkers of myocardial necrosis. In this scenario, the characterization of two troponin T (TnT)-binding aptamers as viable alternative to antibodies employed on clinical immunoassays is here reported for the first time. Their recognition ability was first investigated through surface plasmon resonance (SPR). Subsequently, an enzyme-linked oligonucleotide assay (ELONA) was developed on common 96-well polystyrene plates, both by direct and sandwich detection strategies for comparison. In both cases, the assay exhibits a detection ability of TnT in the range of low nanomolar but a great advantage on serum interference was obtained by using both aptamers in a sandwich format, with excellent reproducibility and recovery values. Despite the sensitivity needing to be enhanced to the low picomolar range, these results are encouraging for the development of new, low-cost, and rapid antibody-free colorimetric assays for AMI studies based on aptamer–Troponin T recognition.
- 13 July 2019
- Published by Springer Nature in Analytical and Bioanalytical Chemistry
- Vol. 411 (29) , 7709-7716
Characterization of troponin T binding aptamers for an innovative enzyme-linked oligonucleotide assay (ELONA)
Early diagnosis of acute myocardial infarction (AMI) is of outmost importance to reduce the mortality rate, and cardiac troponins are considered the gold standard biomarkers of myocardial necrosis. In this scenario, the characterization of two troponin T (TnT)-binding aptamers as viable alternative to antibodies employed on clinical immunoassays is here reported for the first time. Their recognition ability was first investigated through surface plasmon resonance (SPR). Subsequently, an enzyme-linked oligonucleotide assay (ELONA) was developed on common 96-well polystyrene plates, both by direct and sandwich detection strategies for comparison. In both cases, the assay exhibits a detection ability of TnT in the range of low nanomolar but a great advantage on serum interference was obtained by using both aptamers in a sandwich format, with excellent reproducibility and recovery values. Despite the sensitivity needing to be enhanced to the low picomolar range, these results are encouraging for the development of new, low-cost, and rapid antibody-free colorimetric assays for AMI studies based on aptamer–Troponin T recognition.