Shibo Zhang

Vitis vinifera L. is a commercially important horticultural plant with abundant microbial resources. However, the impact of grape-associated microbiota on grape quality and flavor has been largely overlooked. We integrated volatomics and microbiomics to explore temporal variations in berry volatiles and microbial diversity of ‘Cabernet Sauvignon’ in Ningxia (NX) and Shanxi (SX), and the correlation between microbial communities and volatiles. A total of 38 and 35 free and bound aroma compounds, respectively, were identified in NX berries and SX berries. For free aroma, these 38 compounds were classified into aldehydes (69%), alcohols (22%), acids (4%), aromatics (4%), terpenes (0.6%), esters (0.37%), and norisoprenoids (0.3%). Similarly, the 35 bound aromas were attributed to aromatics (58%), acids (29%), terpenes (4%), esters (3%), alcohols (2.82%), aldehydes (2.78%), and norisoprenoids (0.4%). Additionally, a total of 616 bacterial genera and 254 fungal genera were detected in all samples from both regions. The results demonstrated that vineyard sites significantly shaped the characteristics of berry volatiles and microbial biogeographic patterns. SX berries exhibited more abundant free aroma and higher microbial diversity than NX berries, with three key taxa (Sphingomonas, Massilia, and Bacillus) identified in the bacterial network. Correlation analysis results highlighted that these key taxa might play an important role in berry-free aroma. This study reveals the crucial role of microbes in shaping grape flavor and uncovers the link between microbial diversity and the regional attributes of grapes and wine.

Owing to global warming, continuously increasing the grain yield of rainfed maize is challenging on the Loess Plateau in China. Plastic film mulching has been extensively utilized in dryland agriculture on the Loess Plateau. However, higher topsoil temperatures under film mulch caused rainfed-maize premature senescence and yield loss. Here, we aimed to explore the influence of topsoil temperature driven by novel double mulching patterns on rainfed maize productivity based on the excellent moisture conservation function of plastic film. A maize field experiment was conducted in two different areas, namely Changwu, a typical semi-arid area, and Yangling, a dry semi-humid area. The experiment followed a randomized block design with three replications. Five flat-planting practices were examined in 2021 and 2022: (1) bare land (CK), (2) transparent film mulching (PFM), (3) black film mulching (BFM), (4) double mulching of PFM with a black polyethylene net (PFM + BN), and (5) double mulching of PFM with whole maize stalks (PFM + ST). Soil hydrothermal conditions, maize growth dynamics, grain yield, water use efficiency (WUE), and economic returns were quantified under different mulching practices. Under double mulching treatments, topsoil temperatures were lower than PFM by 1.7–2.0 °C at the two sites (p < 0.05), whereas BFM was slightly lower than that of PFM by 0.6–0.7 °C at Yangling (p > 0.05). The average growth period for maize under double mulching was longer than that under PFM by 8–11 days at the two sites. Double mulching treatments significantly improved the leaf area index (LAI), chlorophyll relative content (SPAD), and aboveground biomass compared to CK and PFM during the late growth stage. Compared with PFM, average grain yield increased by 14.93%, 18.46%, and 16.45% in Changwu (p < 0.05) under BFM, PFM + BN, and PFM + ST, respectively, and by 2.71%, 24.55%, and 20.38% in Yangling. The corresponding WUEs also increased. Additionally, net income under BFM was higher than that under other treatments, and there were no significant (p > 0.05) differences between PFM + ST and BFM in Changwu. However, PFM + ST in net income averaged 10.72–52.22% higher than other treatments, and its output value was 19.51% higher in Yangling. In summary, smallholder farmers can adopt PFM + ST to improve rainfed-maize productivity in the Loess Plateau in China.

With the dramatic increased use of agricultural film, the potential environmental risks associated with it have been receiving widespread attention. Biodegradable film (BF) is considered an alternative to conventional polyethylene film (PF), but its feasibility to replace PF needs to be verified. Thus, we conducted a two-year field experiment in the Loess Plateau region of China, exploring the effects of residual biodegradable film and polyethylene film (RBF and RPF) on soil moisture, maize root, and productivity at different residual levels (75 kg ha⁻¹, 150 kg ha⁻¹ and 300 kg ha⁻¹). Regardless of the residual film type, soil water content (SWC), root length density (RLD), and root surface area density (RSD) all decreased with increasing residual level; this phenomenon observed significant differences when the residual level exceeded 150 kg ha⁻¹. Different organs (root and shoot) of maize differed in their sensitivity and sensitivity period to residual film. The two-year degradation rate of RBF was 59.24%, which was higher than that of RPF. Compared to the RPF treatments, the SWC, RLD, RSD, biomass, and root–shoot ratio of the RBF treatments were closer to the no residual film treatment in the second maize growing season. After the two-year experiment, compared to the grain yield, water use efficiency, and precipitation use efficiency of the RPF treatments, that of the RBF treatments increased 0.41–6.24%, 0.12–4.44%, and 0.41–06.24%. The application of BF to replace PF is beneficial to sustainable maize production in dryland, but finding efficient methods to recycle the residual film remains a priority.

Nitrogen (N) is one of the important factors affecting maize root morphological construction and growth development. An association panel of 124 maize inbred lines was evaluated for root and shoot growth at seedling stage under normal N (CK) and low N (LN) treatments, using the paper culture method. Twenty traits were measured, including three shoot traits and seventeen root traits, a genome-wide association study (GWAS) was performed using the Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) methods. The results showed that LN condition promoted the growth of the maize roots, and normal N promoted the growth of the shoots. A total of 185 significant SNPs were identified, including 27 SNPs for shoot traits and 158 SNPs for root traits. Four important candidate genes were identified. Under LN conditions, the candidate gene Zm00001d004123 was significantly correlated with the number of crown roots, Zm00001d025554 was correlated with plant height. Under CK conditions, the candidate gene Zm00001d051083 was correlated with the length and area of seminal roots, Zm00001d050798 was correlated with the total root length. The four candidate genes all responded to the LN treatment. The research results provide genetic resources for the genetic improvement of maize root traits.

In the context of global warming, the effects of warming in the root zone of crops on maize seedling characteristics deserve research attention. Previous studies on the adaptive traits of dryland maize have mainly focused on soil moisture and nutrients, rather than analyzing potential factors for the adaptive traits of root zone warming. This study was conducted to investigate the effects of different root zone warming ranges on the agronomic traits, hormones, and microstructures of maize seedling roots and leaves. The results showed that minor increases in the root zone temperature significantly enhanced maize seedling growth. However, when the temperature in the root zone was excessive, the stem diameter, root surface area, root volume, total root length, dry matter accumulation, and root/shoot biomass of maize seedlings sharply decreased. Under high temperature stress in the root zone, the root conduit area; root stele diameter; root content of trans-zeatin (ZT), gibberellin A₃ (GA₃), and indoleacetic acid (IAA); leaf thickness; upper and lower epidermis thickness; and leaf content of ZT and GA₃ were significantly decreased. The hormone content and microstructure changes might be an important reason for root growth maldevelopment and nutrient absorption blockage, and they also affected the leaf growth of maize seedlings. Compared with the ‘senescent’ maize type Shaandan 902 (SD902), the plant microstructure of the ‘stay-green’ maize type Shaandan 609 (SD609) was less affected by increased temperatures, and the ability of the root system to absorb and transport water was stronger, which might explain its tolerance of high temperature stress in the root zone.

Shoot organogenesis, one of the in vitro plant regeneration processes that occur during in vitro micropropagation, is used in the study of plant development. Morphological, physiological, and molecular aspects of in vitro shoot organogenesis have been extensively studied for over 50 years. Because of the research progress in plant genetics and molecular biology, our understanding of in planta and in vitro shoot meristem development, the cell cycle and cytokinin signal transduction has advanced significantly. These research advances provide useful information as well as molecular tools to study further the genetic and molecular aspects of shoot organogenesis. A number of key molecular markers, genes, and pathways have been shown to play a critical role in the process of in vitro shoot organogenesis. Furthermore, these studies reveal that in vitro shoot organogenesis, as with in planta shoot development, is a complex, well-coordinated developmental process, given that the induction of a single molecular event is likely to be insufficient to induce the entire process. Continued study is required to identify additional molecular events that trigger dedifferentiation and act as developmental switches for de novo shoot development.