Miriam Russ
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Miriam Russ has a total of 14 co-authors. Here are the top co-authors by number of shared publications:
- Astrid Ortner
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Graz, Austria
5 shared publications Reinhold Wintersteiger
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, 8010 Graz, Austria
5 shared publicationsMichaela Andrä
Medical University of Graz
4 shared publicationsSusanne Jauk
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, 8010 Graz, Austria
4 shared publications- Iva Brcic
Diagnostic and Research Institute of Pathology, Medical University of Graz, Graz, Austria
2 shared publications Joachim Greilberger
Medical University of Graz
2 shared publications
Latest Publications
- Open Access
- 31 July 2020
- Published by Wiley in Oxidative Medicine and Cellular Longevity
- Vol. 2020, 1-11
Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts
As progressive organ shortage in cardiac transplantation demands extension of donor criteria, effort is needed to optimize graft survival. Reactive oxygen and nitrogen species, generated during organ procurement, transplantation, and reperfusion, contribute to acute and late graft dysfunction. The combined application of diverse substances acting via different molecular pathways appears to be a reasonable approach to face the complex mechanism of ischemia reperfusion injury. Thus, an antioxidant solution containing α-ketoglutaric acid, 5-hydroxymethylfurfural, N-acetyl-L-methionine, and N-acetyl-selenium-L-methionine was combined with endogenous angiotensin-(1-7). Its capacity of myocardial protection was investigated in isolated Langendorff-perfused rat hearts subjected to warm and cold ischemia. The physiological cardiac parameters were assessed throughout the experiments. Effects were evaluated via determination of the oxidative stress parameters malondialdehyde and carbonyl proteins as well as immunohistochemical and ultrastructural tissue analyses. It was shown that a combination of 20% () antioxidant solution and 220 pM angiotensin-(1-7) led to the best results with a preservation of heart tissue against oxidative stress and morphological alteration. Additionally, immediate cardiac recovery (after warm ischemia) and normal physiological performance (after cold ischemia) were recorded. Overall, the results of this study indicate substantial cardioprotection of the novel combination with promising prospective for future clinical use.- 31 July 2020
- Published by Wiley in Oxidative Medicine and Cellular Longevity
- Vol. 2020, 1-11
Open AccessAntioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat Hearts
As progressive organ shortage in cardiac transplantation demands extension of donor criteria, effort is needed to optimize graft survival. Reactive oxygen and nitrogen species, generated during organ procurement, transplantation, and reperfusion, contribute to acute and late graft dysfunction. The combined application of diverse substances acting via different molecular pathways appears to be a reasonable approach to face the complex mechanism of ischemia reperfusion injury. Thus, an antioxidant solution containing α-ketoglutaric acid, 5-hydroxymethylfurfural, N-acetyl-L-methionine, and N-acetyl-selenium-L-methionine was combined with endogenous angiotensin-(1-7). Its capacity of myocardial protection was investigated in isolated Langendorff-perfused rat hearts subjected to warm and cold ischemia. The physiological cardiac parameters were assessed throughout the experiments. Effects were evaluated via determination of the oxidative stress parameters malondialdehyde and carbonyl proteins as well as immunohistochemical and ultrastructural tissue analyses. It was shown that a combination of 20% () antioxidant solution and 220 pM angiotensin-(1-7) led to the best results with a preservation of heart tissue against oxidative stress and morphological alteration. Additionally, immediate cardiac recovery (after warm ischemia) and normal physiological performance (after cold ischemia) were recorded. Overall, the results of this study indicate substantial cardioprotection of the novel combination with promising prospective for future clinical use. - Open Access
- 24 July 2019
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 461 (1-2) , 73-80
Investigation of antioxidative effects of a cardioprotective solution in heart tissue
A multi-component solution, containing α-ketoglutaric acid (α-KG), 5-hydroxymethylfurfural (5-HMF), N-acetyl-seleno-l-methionine (NASeLM), and N-acetyl-l-methionine (NALM) as active ingredients, has been tested considering its supposed antioxidative effect with respect to heart transplantations. Oxidative stress was induced on isolated rat hearts through occlusion of a coronary artery and in chicken heart tissue through hydrogen peroxide. Both heart types were analyzed and the oxidative stress markers malondialdehyde (MDA) and carbonyl proteins (CPs) were determined via HPLC/UV–Vis. In both approaches, it was found that treatment with the multi-component solution led to a lower amount of MDA and CPs compared to a negative control treated with Krebs–Ringer solution (KRS). Further investigation on chicken heart tissue identified α-KG as antioxidative component in these experiments. However, numerous factors like arrhythmia, vessel dilatation, and minimization of oxidative stress effects play an important role for successful transplantation. Therefore, the investigated multi-component solution might be a novel approach against oxidative stress situations, for example at ischemia reperfusion injury during heart transplantations.
- 24 July 2019
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 461 (1-2) , 73-80
Open AccessInvestigation of antioxidative effects of a cardioprotective solution in heart tissue
A multi-component solution, containing α-ketoglutaric acid (α-KG), 5-hydroxymethylfurfural (5-HMF), N-acetyl-seleno-l-methionine (NASeLM), and N-acetyl-l-methionine (NALM) as active ingredients, has been tested considering its supposed antioxidative effect with respect to heart transplantations. Oxidative stress was induced on isolated rat hearts through occlusion of a coronary artery and in chicken heart tissue through hydrogen peroxide. Both heart types were analyzed and the oxidative stress markers malondialdehyde (MDA) and carbonyl proteins (CPs) were determined via HPLC/UV–Vis. In both approaches, it was found that treatment with the multi-component solution led to a lower amount of MDA and CPs compared to a negative control treated with Krebs–Ringer solution (KRS). Further investigation on chicken heart tissue identified α-KG as antioxidative component in these experiments. However, numerous factors like arrhythmia, vessel dilatation, and minimization of oxidative stress effects play an important role for successful transplantation. Therefore, the investigated multi-component solution might be a novel approach against oxidative stress situations, for example at ischemia reperfusion injury during heart transplantations.
- Open Access
- 12 October 2018
- Published by Springer Nature in International Journal of Peptide Research and Therapeutics
- Vol. 25 (4) , 1271-1278
Stabilization of Angiotensin-(1-7) in Cardioprotective Solutions
- 12 October 2018
- Published by Springer Nature in International Journal of Peptide Research and Therapeutics
- Vol. 25 (4) , 1271-1278
Open AccessStabilization of Angiotensin-(1-7) in Cardioprotective Solutions
- Open Access
- 20 September 2018
- Published by Taylor & Francis in Analytical Letters
- Vol. 52 (6) , 892-901
Characterization of Oxidative Stress in Chicken Heart Tissue by High-Performance Liquid Chromatography with Ultraviolet‐Visible (HPLC–UV–Vis) Detection
Oxidative stress can lead to tissue damage in the body, resulting in conditions that may lead to heart failure. Carbonyl proteins and malondialdehyde are common markers for determining oxidative stress. In this study, a high-performance liquid chromatography with ultraviolet‐visible (HPLC–UV–Vis) method was developed for both carbonyl proteins and malondialdehyde and compared with common spectrophotometric methods. The HPLC–UV–Vis method for determining carbonyl proteins showed a linear correlation in a range of 0.05–1.6 µg.mL−1 (correlation coefficient: 0.9939). The limits of detection and quantitation were determined to be 1.7 and 5.1 pmol on-column, respectively. The HPLC–UV–Vis method for malondialdehyde exhibited a linear correlation in the range of 0.04–13.6 µg.mL−1 (correlation coefficient: 0.9982) with limits of detection and quantitation of 1.5 and 4.5 pmol on-column, respectively. As a proof of concept, the applicability of the methods for both marker substances was investigated in chicken heart tissue as a complex matrix sample. To simulate oxidative stress, the tissue was treated with hydrogen peroxide as the oxidizing agent. Samples prepared with hydrogen peroxide showed increased carbonyl protein and malondialdehyde levels when compared to non-oxidized samples and samples treated additionally with the antioxidant Trolox. Regarding oxidative stress, similar results were achieved when compared with common spectrophotometric methods. However, HPLC–UV–Vis presented higher reproducibility and accuracy. The developed methods for both markers enable rapid and simple investigation of possible antioxidant effects on tissue samples.
- 20 September 2018
- Published by Taylor & Francis in Analytical Letters
- Vol. 52 (6) , 892-901
Open AccessCharacterization of Oxidative Stress in Chicken Heart Tissue by High-Performance Liquid Chromatography with Ultraviolet‐Visible (HPLC–UV–Vis) Detection
Oxidative stress can lead to tissue damage in the body, resulting in conditions that may lead to heart failure. Carbonyl proteins and malondialdehyde are common markers for determining oxidative stress. In this study, a high-performance liquid chromatography with ultraviolet‐visible (HPLC–UV–Vis) method was developed for both carbonyl proteins and malondialdehyde and compared with common spectrophotometric methods. The HPLC–UV–Vis method for determining carbonyl proteins showed a linear correlation in a range of 0.05–1.6 µg.mL−1 (correlation coefficient: 0.9939). The limits of detection and quantitation were determined to be 1.7 and 5.1 pmol on-column, respectively. The HPLC–UV–Vis method for malondialdehyde exhibited a linear correlation in the range of 0.04–13.6 µg.mL−1 (correlation coefficient: 0.9982) with limits of detection and quantitation of 1.5 and 4.5 pmol on-column, respectively. As a proof of concept, the applicability of the methods for both marker substances was investigated in chicken heart tissue as a complex matrix sample. To simulate oxidative stress, the tissue was treated with hydrogen peroxide as the oxidizing agent. Samples prepared with hydrogen peroxide showed increased carbonyl protein and malondialdehyde levels when compared to non-oxidized samples and samples treated additionally with the antioxidant Trolox. Regarding oxidative stress, similar results were achieved when compared with common spectrophotometric methods. However, HPLC–UV–Vis presented higher reproducibility and accuracy. The developed methods for both markers enable rapid and simple investigation of possible antioxidant effects on tissue samples.
- 9 October 2015
- Published by Springer Nature in Journal of Fluorescence
- Vol. 26 (1) , 81-86
Determination of Angiotensin-(1–7) with HPLC/Fluorescence-Detection
- 9 October 2015
- Published by Springer Nature in Journal of Fluorescence
- Vol. 26 (1) , 81-86
Determination of Angiotensin-(1–7) with HPLC/Fluorescence-Detection