Juan Francisco Toro-Fernandez
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Juan Francisco Toro-Fernandez has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
- Adela López-García
Digestive and General Surgery Service, Principe de Asturias University Hospital, 28015 Madrid, Spain
1 shared publication - Alberto Gutiérrez-Calvo
Hospital Universitario Príncipe de Asturias, General and Digestive Surgery Department, Alcalá de Henares, Spain
1 shared publication - Christian Vaquero-Yuste
Department of Immunology, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain
1 shared publication - Ignacio Juarez
Departamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de Madrid, Spain
1 shared publication - Inmaculada Lasa-Unzúe
Hospital Universitario Príncipe de Asturias, General Surgery, Madrid, Spain
1 shared publication - José Manuel Martín-Villa
Departamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain
1 shared publication
Latest Publications
- Open Access
A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer
We have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a housekeeping gene by conventional PCR and the densitometric analysis of the bands obtained. We established a cut-off point based on the mean and standard deviation analyzing the DNA of 30 gastric tissues from patients undergoing non-cancer gastrectomy. diffPCR and qPCR yielded consistent results. HER2-overexpression was detected in 25% of patients and was further confirmed by immunohistochemistry and immunofluorescence. The approaches herein described may serve as complementary and reliable methods to assess HER2 amplification.
Open AccessA Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer
We have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a housekeeping gene by conventional PCR and the densitometric analysis of the bands obtained. We established a cut-off point based on the mean and standard deviation analyzing the DNA of 30 gastric tissues from patients undergoing non-cancer gastrectomy. diffPCR and qPCR yielded consistent results. HER2-overexpression was detected in 25% of patients and was further confirmed by immunohistochemistry and immunofluorescence. The approaches herein described may serve as complementary and reliable methods to assess HER2 amplification.