Kazuaki Tanaka

Zoological Science publishes articles, reviews and editorials that cover the broad and increasingly interdisciplinary field of zoology.

Sentence-final particles serve an essential role in spoken Japanese because they express the speaker's mental attitudes toward a proposition and/or an interlocutor. They are acquired at early ages and occur very frequently in everyday conversation. However, there has been little proposal for a computational model of acquiring sentence-final particles. This paper proposes Subjective BERT, a self-attention model that takes various subjective senses in addition to language and images as input and learns the relationship between words and subjective senses. An evaluation experiment revealed that the model understands the usage of "yo", which expresses the speaker's intention to communicate new information, and that of "ne", which denotes the speaker's desire to confirm that some information is shared.

Copy number variation of amylase alpha 2B gene is associated with feed efficiency traits in Large White pigs--title not given-- | Tsukasa Yoshidomi, Kazuaki Tanaka, Tatsuya Takizawa, Satoshi Nikaido, Tetsuya Ito, Mai Kamikawa, Kensuke Hirose | Agricultural Journals

Detection of copy number variation of alpha amylase genes in domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa) | Tsukasa Yoshidomi, Kensuke Hirose, Takeshi Kuge, Yukio Okada, Yaetsu Kurosawa, Tatsuya Takizawa, Kazuaki Tanaka | Agricultural Journals

The majority of dairy cows in Japan are Holstein cows. Milk items containing only milk from Jersey cows are sold at a premium price. Therefore, it is important to keep Holstein milk from contaminating the milk labeled as pure Jersey milk to maintain brand value. Here, genetic testing was conducted on packaged milk items. Polymorphisms of the melanocortin-1 receptor (MC1R) gene, which controls variations in coat color, were assayed in addition to the frequencies of MC1R alleles in Holstein and Jersey cows in Japan. In Holstein cows, the frequency of the E^D allele was 0.876, but this allele is absent in Jersey cows. Thus, detection of the E^D allele clearly indicates the presence of Holstein DNA. This DNA-based test was also useful in testing sterilized milk products. The results of the DNA-based test show that pure Jersey milk can be easily distinguished from Holstein milk.

Sika deer (Cervus nippon) in Japan are classified into southern and northern groups. However, previous studies primarily relied on maternally inherited mitochondrial DNA (mtDNA). The paternally inherited Y-chromosome is useful for analyzing the contribution of males to the population genetic history of sika deer. In total, approximately 16 kb of partial sequences of four Y-chromosomal genes, Y-linked, sex-determining region Y, DEAD-box helicase 3 Y-linked, and Zinc finger protein Y-linked, were sequenced to investigate intraspecific variation. As a result, we identified nine intronic single nucleotide polymorphisms (SNPs) in 478 sika deer samples collected over the entire Japanese archipelago from Hokkaido to Kyushu. SNP genotyping revealed 10 distinct haplotypes (SYH1–SYH10). The most common haplotype (SYH1) was present in all populations and was the most abundant haplotype, identified in 80.3% of the sampled individuals. The remaining haplotypes were unique to a single locality. SYH1 was also central to all other haplotypes that diverged by a SNP, resulting in this haplotype being the core of a star-like cluster topography. We found that contrary to mtDNA patterns, there was no clear differentiation of Y-chromosome markers between the southern and the northern populations. Due to the female philopatry of sika deer, mtDNA may provide a highly structured differentiation of populations. On the other hand, the male-biased gene flow may provide a reduced differentiation of populations. Our findings revealed that the genetic structure of the Japanese sika deer is more complex than previously thought based on mtDNA-based phylogeographic studies.

Interleukin-4 (IL4) and interleukin-13 (IL13) are involved in the initial response of T helper 2 lymphocytes through the activation of the IL4 receptor alpha (IL4RA), which is a common receptor chain for these cytokines. In humans, several single-nucleotide polymorphisms (SNPs) identified in the IL4R and in interleukin coding genes were associated with atopic disorders. However, the association between canine IL4R polymorphisms and atopic disorders has not been investigated yet. This study aimed to determine the associations between four non-synonymous SNPs and canine atopic dermatitis (CAD) in shiba inu and miniature dachshund populations. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to genotype four polymorphisms of canine IL4R and IL13 in 34 shiba inu and 19 miniature dachshund patients with CAD, as well as 29 shiba inu and 39 miniature dachshund patients without the condition. Results from miniature dachshunds revealed a potential association between the presence of minor A allele rs24378020 and CAD (odds ratio, 0.10; 95% confidence interval, 0.01–0.85; poriginal=0.0062). This CAD resistance allele led to an amino acid substitution (Arg688Cys) that could impair IL4 and IL13 signaling. In shiba inu patients, rs24378020 was fixed by homozygosity of the major G allele. No association was found between the remaining three evaluated SNPs and CAD. Nevertheless, the study suggests that the IL4R Cys688 variant reduces the risk of CAD in miniature dachshunds.

To develop a noninvasive sample collection method for genotyping, we compared PCR products from samples collected from neonates using five different brands of adhesive tape. Next, the youngest application age to distinguish genotypes was established. The tapes were applied on the backs of rats on postnatal day (PND) 10. DNA extracts from two brands provided clear PCR products that enabled genotype identification. The youngest age for distinguishing genotypes was PND 5; however, the youngest age that provided accurate results was PND 7. Thus, the present method allows for genotyping during the neonatal period without invasive burden and may improve animal welfare by refining.

Phase-contrast microscopy is a convenient technique for live-cell observation on the surface of materials with high optical transmittance. Here, we demonstrate a novel technique to observe living cells on the surface of materials with low optical transmittance, such as polytetrafluoroethylene (PTFE), which are widely used in biomaterials for blood-contacting devices. The surface of a cover glass was coated with a thin PTFE layer with sufficient transmittance, thereby enabling the observation of living cells on the PTFE surface with a phase-contrast microscope.