A. M. Mendybayeva

Background and Aim. Infectious pathologies are prevalent among canines, with the majority being of viral etiology. The most notable examples include carnivore plague and adenovirus infection. A distinctive attribute of viral infections is their tendency to occur in clusters, whereas monoinfections are observed with a lower frequency. Clinically, high mortality rates are frequently observed in viral diseases, often due to the development of secondary infections that complicate the course of the primary disease. The objective of the present study was to investigate the clinical manifestations (symptoms, haematological and biochemical blood parameters) of viral diseases (i.e. canine plague and adenovirus infection) in dogs complicated by opportunistic microorganisms, in the conditions of Kostanay city, RK. Materials and Methods. Canines of various breeds, sexes and age groups were utilised as subjects for the study. The diagnosis of viral diseases in canines was conducted in a comprehensive manner. Dogs with confirmed viral infection were sampled for the isolation and identification of opportunistic microorganisms. Results. In Kostanay city, from 2022 to 2024, 15 cases of plague of carnivores and 12 cases of adenovirus infection were registered, 13 isolates of opportunistic microorganisms were detected and identified. Blood analysis in monoinfection showed decreased erythrocyte count, haemoglobin and platelet levels. In adenovirus type CAV-1, there were abnormalities in total bilirubin, ALT, AST alkaline phosphotase, and albumin. Blood analysis in viral diseases complicated by associations of opportunistic microorganisms showed leukocytosis, increased neutrophils, globulins and urea indicating the development of secondary infection. Conclusion. The combination of clinical symptoms and laboratory data allow a more accurate assessment of the severity of the animal's condition and identify lesions of various body systems. The prevalence of opportunistic microorganisms in viral pathologies aggravate the course of the main disease and cause the development of secondary infection.

Background and Aim. Meat is a favourable environment for the growth and viability of pathogenic microorganisms. Bacteria in meat cause spoilage and increase the risk of foodborne toxic infections of various origins during consumption. The importance of monitoring and analysing microbial contamination is increasing due to increased requirements for food safety and public health protection. The aim of the study was to analyse the overall level of microbial contamination of different types of meat sampled from retail outlets in the Kostanay region.Materials and Methods. A total of 30 meat samples, including pork, beef, and horse meat, were collected for analysis. To assess microbial contamination of the meat, the number of mesophilic aerobic and facultatively anaerobic microorganisms (QMAFAnM) and their species composition were determined.Results. It was found that 96% of the meat sold at the retail level had elevated QMAFAnM, with coliforms isolated in 30% of the samples. Salmonella spp. and Listeria monocytogenes were not detected in the meat tested. Pork meat had the highest microbial contamination compared to beef and horse meat samples.Conclusion. Exceeding the permitted levels of QMAFAnM and the presence of coliform bacteria indicate potential health risks for consumers and more effective measures are required to ensure food safety.

Parvovirus enteritis of dogs is the cause of a highly contagious acute disease of many carnivorous animals, which leads to severe gastroenteritis and myocarditis. Parvovirus infection is characterized by severe enteritis and vomiting, as well as dehydration, fever, leukopenia and diarrhea. The article presents data on the frequency of occurrence of the disease in dogs with parvovirus enteritis in Kostanay, the Republic of Kazakhstan. In order to assess the epizootological situation of parvovirus enteritis, the logs of registration of sick animals, the results of rapid tests for infectious diseases of dogs of private veterinary clinics of the city for the period from 2020 to 2023 were analyzed. The object of the study was dogs of different breeds and age groups. Data analysis has shown that parvovirus enteritis is the most common infectious diseases of dogs. It was diagnosed in 20.3% of veterinary clinic patients, in two forms of intestinal and myocarditis. The disease is seasonal. Infection of dogs with parvovirus occurs mainly in spring (35,6%) and autumn (37,8%). The analysis of the sex and age structure of patients with parvovirus enteritis indicates a greater susceptibility to the disease of puppies under the age of one year. 86% of patients belonged to this age group. Dogs get sick regardless of gender. Parvovirus enteritis of dogs is the cause of a highly contagious acute disease of many carnivorous animals, which leads to severe gastroenteritis and myocarditis. Parvovirus infection is characterized by severe enteritis and vomiting, as well as dehydration, fever, leukopenia and diarrhea. The article presents data on the frequency of occurrence of the disease in dogs with parvovirus enteritis in Kostanay, the Republic of Kazakhstan. In order to assess the epizootological situation of parvovirus enteritis, the logs of registration of sick animals, the results of rapid tests for infectious diseases of dogs of private veterinary clinics of the city for the period from 2020 to 2023 were analyzed. The object of the study was dogs of different breeds and age groups. Data analysis has shown that parvovirus enteritis is the most common infectious diseases of dogs. It was diagnosed in 20.3% of veterinary clinic patients, in two forms of intestinal and myocarditis. The disease is seasonal. Infection of dogs with parvovirus occurs mainly in spring (35,6%) and autumn (37,8%). The analysis of the sex and age structure of patients with parvovirus enteritis indicates a greater susceptibility to the disease of puppies under the age of one year. 86% of patients belonged to this age group. Dogs get sick regardless of gender. Parvovirus enteritis of dogs is the cause of a highly contagious acute disease of many carnivorous animals, which leads to severe gastroenteritis and myocarditis. Parvovirus infection is characterized by severe enteritis and vomiting, as well as dehydration, fever, leukopenia and diarrhea. The article presents data on the frequency of occurrence of the disease in dogs with parvovirus enteritis in Kostanay, the Republic of Kazakhstan. In order to assess the epizootological situation of parvovirus enteritis, the logs of registration of sick animals, the results of rapid tests for infectious diseases of dogs of private veterinary clinics of the city for the period from 2020 to 2023 were analyzed. The object of the study was dogs of different breeds and age groups. Data analysis has shown that parvovirus enteritis is the most common infectious diseases of dogs. It was diagnosed in 20.3% of veterinary clinic patients, in two forms of intestinal and myocarditis. The disease is seasonal. Infection of dogs with parvovirus occurs mainly in spring (35,6%) and autumn (37,8%). The analysis of the sex and age structure of patients with parvovirus enteritis indicates a greater susceptibility to the disease of puppies under the age of one year. 86% of patients belonged to this age group. Dogs get sick regardless of gender.

This article reveals the features of the resistance of Staphylococcus аureus strains isolated from animals, birds and animal products in the territory of Kostanay region. Staphylococci can infect any tissue or organ of an animal, causing more than 100 different diseases. According to literary sources, more than a dozen species of non-pathogenic or conditionally pathogenic staphylococci are isolated from many agricultural and domestic animals and birds. For the first time in the Kostanay region, the analysis of antibiotic resistance of staphylococcus strains isolated in livestock farms and animal products was carried out.The analysis of antibiotic resistance of staphylococcus strains isolated in livestock farms of Kostanay region and animal products was carried out. The study showed that the largest number of isolates of Staphylococcus аureus showed high resistance to beta-lactam antibiotics by an average of 55.3%, fluoroquinolones by 47.1% and tetracyclines by 45.6%.Of the 69 studied isolates of Staphylococcus аureus, antibiotic resistance was shown by 43 (62.3%) isolates, 26 (37.7%) isolates were sensitive to all groups of AMD.It was found that the largest number of isolates is 62.3% in the group of beta-lactams resistant to various antibiotics, the least resistant strains are 7.9% in the group of aminoglycosides and 20.3% in the group of sulfonamides.

One of the main problems in the treatment of infectious diseases in pets is the spread of strains of microorganisms resistant to antimicrobial drugs. The aim of the study was to isolate conditionally pathogenic microorganisms of the Enterobacteriaceae family from dogs with parvavirus enteritis and clinically healthy animals, with the determination of phenotypic and genotypic resistance to antimicrobial drugs. In the period from March 2021 to March 2023, biological material from dogs with parvavirus enteritis (n = 152) and healthy dogs (n = 196), taken in veterinary clinics of Kostanay, was analyzed. Eighty-two isolates of conditionally pathogenic microorganisms of the Enterobacteriaceae family were isolated and studied from 348 biomaterial samples taken from dogs with enteritis. Conditionally pathogenic Enterobacteriaceae were isolated in 26.3% of cases (40 strains), among them: 65% of E.coli strains, 12% - Klebsiella, 10% - Proteus, 8% - Citrobacter, 5%- Enterobacter. Forty two microorganisms were isolated from healthy dogs: 83% are E.coli microorganisms, 5% are Klebsiella and Proteus isolates, and 2% are Citrobacter and Enterobacter microorganisms. In all isolated isolates, the resistance/sensitivity to the action of antibacterial drugs was determined by the disco-diffuse method. It was revealed that all isolated strains of microorganisms showed sensitivity to the action of streptomycin, belonging to the group of aminoglycosides, showed resistance to tetracycline, doxycycline, ofloxacin, ampicillin, amoxicillin. Resistance genes have been identified to beta-lactams, aminoglycosides, tetracyclines, and sulfonamides. The resistance gene to fluoroquinolones was isolated from 1 E. coli isolate, from a healthy animal. Resistance genes were not detected in the DNA of Citrobacter, Enterobacter and Proteus isolates isolated from clinically healthy dogs. The most common genes were genes encoding resistance to aminoglycosides - 28% of strains, to beta-lactams - 21.9%, to tetracyclines - 13.4% of animals. It was concluded that the uncontrolled and frequent use of antibacterial drugs of the beta-lactam group and tetracyclines in dogs leads to the spread of genotypic resistance among microorganisms of the Enterobacteriaceae family.

Background and Aim: Salmonella is one of the main causative agents of foodborne infections. The source of the pathogen, in most cases, is poultry products. The intensification of poultry farming and the constant and uncontrolled use of antimicrobials has led to an increase in the level of antibiotic resistance, especially in developing countries. This study aimed to determine the level of sensitivity to antimicrobial agents in Salmonella enterica strains isolated from poultry products in Northern Kazakhstan, as well as to determine the genetic mechanisms of resistance and the presence of integrons. Materials and Methods: In total, 398 samples of poultry products sold in Northern Kazakhstan were selected. Salmonella strains were isolated from product samples using microbiological methods. Salmonella was identified based on morphological, biochemical, and serological methods, as well as polymerase chain reaction (PCR). Sensitivity testing for antimicrobial agents was performed using the disk diffusion method. The detection of resistance genes was performed using PCR and gel electrophoresis. Results: Out of 398 samples of poultry products, a total of 46 Salmonella isolates were obtained. Most of the isolates belong to the serovar Salmonella Enteritidis (80.4%). The assessment of sensitivity to antibacterial agents showed that Salmonella was mainly resistant to nalidixic acid (63%), furadonin (60.9%), ofloxacin (45.6%), and tetracycline (39.1%). In 64.3% of cases, Salmonella was resistant to three or more groups of antibacterial agents. Resistance genes such as tetA, tetB, blaTEM, aadA, sul3, and catII, as well as integrons of two classes (teg1 and teg2), were identified. Conclusion: Poultry products contain antimicrobial-resistant strains of Salmonella, as well as genes encoding resistance mechanisms. The results emphasize the need for constant monitoring of not only pathogenic microorganisms but also their sensitivity to antimicrobial agents. The potential threat to human health requires a unified approach to the problem of antibiotic resistance from representatives of both public health and the agroindustrial complex. Keywords: antibiotic resistance, food safety, poultry, resistance genes, Salmonella.

The widespread use of antimicrobial agents for pets in veterinary practice has led to the emergence and spread of drug resistance not only in pathogenic but also in opportunistic bacteria. The study aims to evaluate the profile of resistance to antibacterial agents in Escherichia coli strains isolated from domestic cats and dogs, as well as to look into the determinants of resistance responsible for the ability of microorganisms to resist the effects of antimicrobial agents. During 2021-2022, biological material from cats and dogs, taken in veterinary clinics of Kostanay, Republic of Kazakhstan, was analyzed. Identification of genes encoding antimicrobial resistance was carried out by Polymerase Chain Reaction (PCR). As a result, it was found that all isolated strains of microorganisms showed sensitivity to the action of meropenem included in the group of beta-lactam antibiotics and showed resistance to tetracycline, doxycycline, ofloxacin, ampicillin, and amoxicillin. Resistance genes were identified for all the studied groups of antibiotics in the DNA of E. coli isolated from cats and no genes encoding resistance to fluoroquinolones were detected in the DNA of strains isolated from dogs. The most common genes were genes encoding resistance to aminoglycosides (43.8%), tetracyclines (35%), beta-lactams (33.5%), sulfonamides (16.1%), and fluoroquinolones (1.24%). In general, domestic cats and dogs have a high prevalence of E. coli strains resistant to beta-lactam drugs (ampicillin: 40%, amoxicillin: 36%) and tetracyclines (tetracycline: 71%, doxycycline: 47%). Resistance in most cases was explained by the presence of such resistance genes as blaTEM, OXA, tetA, and tetB.