Qi Zhang
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Qi Zhang has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Alexander A. Green
Boston University
1 shared publication Duo Ma
Biodesign Center for Molecular Design and Biomimetics, The Biodesign Institute and School of Molecular Sciences, Arizona State University, Tempe, USA
1 shared publicationFuqing Wu
School of Biological and Health Systems Engineering, Arizona State University, Tempe, USA
1 shared publicationKaiyue Wu
Department of Biomedical Engineering, Boston University, Boston, USA
1 shared publicationKylie Standage-Beier
School of Biological and Health Systems Engineering, Arizona State University, Tempe, USA
1 shared publication- Xiao Wang
Arizona State University
1 shared publication
Latest Publications
- 21 June 2021
- Published by Springer Nature in Nature Chemical Biology
- Vol. 17 (7) , 828-836
Predictable control of RNA lifetime using engineered degradation-tuning RNAs
The ability to tune RNA and gene expression dynamics is greatly needed for biotechnological applications. Native RNA stabilizers or engineered 5′ stability hairpins have been used to regulate transcript half-life to control recombinant protein expression. However, these methods have been mostly ad hoc and hence lack predictability and modularity. Here, we report a library of RNA modules called degradation-tuning RNAs (dtRNAs) that can increase or decrease transcript stability in vivo and in vitro. dtRNAs enable modulation of transcript stability over a 40-fold dynamic range in Escherichia coli with minimal influence on translation initiation. We harness dtRNAs in messenger RNAs and noncoding RNAs to tune gene circuit dynamics and enhance CRISPR interference in vivo. Use of stabilizing dtRNAs in cell-free transcription-translation reactions also tunes gene and RNA aptamer production. Finally, we combine dtRNAs with toehold switch sensors to enhance the performance of paper-based norovirus diagnostics, illustrating the potential of dtRNAs for biotechnological applications.
- 21 June 2021
- Published by Springer Nature in Nature Chemical Biology
- Vol. 17 (7) , 828-836
Predictable control of RNA lifetime using engineered degradation-tuning RNAs
The ability to tune RNA and gene expression dynamics is greatly needed for biotechnological applications. Native RNA stabilizers or engineered 5′ stability hairpins have been used to regulate transcript half-life to control recombinant protein expression. However, these methods have been mostly ad hoc and hence lack predictability and modularity. Here, we report a library of RNA modules called degradation-tuning RNAs (dtRNAs) that can increase or decrease transcript stability in vivo and in vitro. dtRNAs enable modulation of transcript stability over a 40-fold dynamic range in Escherichia coli with minimal influence on translation initiation. We harness dtRNAs in messenger RNAs and noncoding RNAs to tune gene circuit dynamics and enhance CRISPR interference in vivo. Use of stabilizing dtRNAs in cell-free transcription-translation reactions also tunes gene and RNA aptamer production. Finally, we combine dtRNAs with toehold switch sensors to enhance the performance of paper-based norovirus diagnostics, illustrating the potential of dtRNAs for biotechnological applications.