Xianrui Xie

Conjugate electrospun fully inorganic SiO ₂ /kaolin 3D nanofiber sponges for rapid hemostasis.

Extraction sockets are prone to bacterial colonization, which causes alveolar bone resorption and refractory infection. However, conventional biomaterials cannot eliminate pathogenic bacteria and modulate the oral immune microenvironment at the same time. Herein, we constructed a structurally tailored hydrogel (HAQM) with in-network bactericidal activity to achieve mild photothermal synergistic sterilization. Mild hyperthermia (∼42 °C) increases bacterial membrane fluidity and strengthens the contact-killing performance of the hydrogel. In vitro and in vivo biological assays verify its outstanding antibacterial activity, particularly against oral obligate anaerobes, with an antibacterial efficiency reaching 95%. Transcriptomic analysis reveals that the hydrogel destabilizes bacterial membranes, disturbs bacterial metabolic pathways, and suppresses bacterial envelope repair. Benefiting from its interconnected porous architecture and redox-responsive activity, the hydrogel mitigates inflammatory response and promotes M2-type macrophage polarization. This multifunctional integrated platform provides an innovative and clinically translatable therapeutic strategy for the repair of infected oral bone defects.

Charred Sanguisorbae Radix (CSR) is a well-known traditional Chinese medicine clinically used to treat conditions such as burns and bleeding, with tannins being its main active ingredients. In this study, the tannins (SDT) were isolated and purified from CSR using macroporous resin. Subsequently, SDT was loaded onto a poly(lactic acid) (PLA) nanofiber membrane using electrospinning, and its potential application value as a wound dressing was evaluated. The experimental results demonstrated that the nanofiber membrane loaded with SDT exhibited strong antioxidant activity and favorable antibacterial performance, both of which were enhanced with increasing SDT concentration. Among the tested membranes, the PLA2 membrane with 9% SDT loading (90 mg SDT per 1 g PLA, mass ratio) exhibited excellent biocompatibility and could significantly promote wound closure of L929 fibroblasts in the cell scratch assay. Furthermore, the PLA2 membrane could slowly release gallic acid to continuously exert its therapeutic effect. In summary, the novel SDT-loaded PLA nanofiber membrane shows great potential as a wound dressing, providing a scientific foundation for the further application of CSR in wound healing therapy.

Introduction: Wound infection remains significant challenge in clinical healing, frequently leading to delayed tissue regeneration and prolonged inflammatory responses. Methods: Herein, a bioinspired electrospun Janus nanofibrous dressing (QAS-PU/FC) was designed to mimic the hierarchical architecture of natural skin and achieve synergistic infection inhibition and tissue regeneration. Results: The dressing is characterized by an Janus structure, in which the outer layer, composed of quaternary ammonium acrylate-based polyurethane and polycaprolactone (PCL), provides robust antibacterial protection and mechanical integrity, while the inner layer, composed of collagen and PCL, offers a biocompatible matrix that supports cell adhesion, proliferation, and tissue remodeling. The Janus design creates a functionally graded interface, enabling sequential antibacterial defense and regenerative stimulation. The outer antibacterial surface effectively inhibits both Staphylococcus aureus and Escherichia coli colonization, while the inner collagen-rich layer promotes fibroblast migration and neovascularization. In vivo studies further demonstrated that the Janus dressing significantly suppressed bacterial infection, reduced inflammation, and accelerated wound closure, achieving nearly complete tissue regeneration with organized collagen deposition and re-epithelialization. Discussion: This work provides a promising bioinspired strategy to design multifunctional Janus electrospun membranes for infection-controlled and regenerative wound care applications.

Chronic infected wounds remain challenging to treat, primarily due to persistent bacterial infection and dysregulated inflammation. Conventional dressings with physically blended bioactives suffer from burst release and unstable retention. Herein, curcumin (Cur) and a dihydroxy-quaternary ammonium salt (Qas) were covalently linked to polylactic acid (PLA) (forming PCur and PQas). This strategy eliminated burst release, enabling sustained bioactive release for 21 days (vs PQas > physical blends > PLA) underscores the critical role of covalent conjugation in balancing antimicrobial, antioxidant, and regenerative functions for prolonged wound therapy. Covalent conjugation is key for balanced functions, offering a sustained therapy for chronically infected wounds.