Hsin-Chih Lai
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Hsin-Chih Lai has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
Jia-Hurng Liu
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taiwan, ROC
1 shared publicationJwu-Ching Shu
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan (ROC)
1 shared publicationKwen-Tay Luh
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan, ROC
1 shared publicationMeng-Jiun Lai
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan (ROC)
1 shared publicationPo-Chi Soo
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan (ROC)
1 shared publicationShen-Wu Ho
School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan (ROC)
1 shared publication
Latest Publications
- 1 November 2000
- Published by Springer Nature in Journal of Biomedical Science
- Vol. 7 (6) , 475-483
Role offlhDC in the expression of the nuclease genenucA, cell division and flagellar synthesis inserratia marcescens
We investigated inSerratia marcescens the functions of theflhDC operon, which controls motility and cell division in enteric bacteria. Included in our evaluations were investigation of cell division, flagellar synthesis and regulation of the expression of nuclease (encoded by thenucA Sm gene, one of the virulence factors). Interruption of the chromosomalflhDC operon inS. marcescens CH-1 resulted in aberrant cell division and loss of nuclease and flagella. Expression ofnucA Sm and other mutated phenotypes was restored in theflhDC mutant by the induction of overexpression offlhDC in a multicopy plasmid. MulticopiedflhDC also induced the formation of differentiated cells (polyploid aseptate cells with oversynthesis of peritrichous flagella) in broth culture using minimal growth medium. Expression of theflhDC operon showed positive autoregulation, and was growth phase dependent (upregulated in early log phase). In addition,flhDC expression was inhibited when the temperature increased from 30 to 37°C, and when osmolarity was increased, but was not influenced by glucose catabolite repression. These results show that FlhD/FlhC is a multifunctional transcriptional activator involved in the process of cell differentiation, swarming and virulence factor expression.
- 1 November 2000
- Published by Springer Nature in Journal of Biomedical Science
- Vol. 7 (6) , 475-483
Role offlhDC in the expression of the nuclease genenucA, cell division and flagellar synthesis inserratia marcescens
We investigated inSerratia marcescens the functions of theflhDC operon, which controls motility and cell division in enteric bacteria. Included in our evaluations were investigation of cell division, flagellar synthesis and regulation of the expression of nuclease (encoded by thenucA Sm gene, one of the virulence factors). Interruption of the chromosomalflhDC operon inS. marcescens CH-1 resulted in aberrant cell division and loss of nuclease and flagella. Expression ofnucA Sm and other mutated phenotypes was restored in theflhDC mutant by the induction of overexpression offlhDC in a multicopy plasmid. MulticopiedflhDC also induced the formation of differentiated cells (polyploid aseptate cells with oversynthesis of peritrichous flagella) in broth culture using minimal growth medium. Expression of theflhDC operon showed positive autoregulation, and was growth phase dependent (upregulated in early log phase). In addition,flhDC expression was inhibited when the temperature increased from 30 to 37°C, and when osmolarity was increased, but was not influenced by glucose catabolite repression. These results show that FlhD/FlhC is a multifunctional transcriptional activator involved in the process of cell differentiation, swarming and virulence factor expression.