Clara Puig-Herreros

Currently, there is no consensus on the characterization of the human voice. The objective of the present study is to describe the myoelectric behavior of the extrinsic musculature of the larynx in 146 people with normal voice (Spanish speakers), aged between 20 and 50 years old. Different vocal tasks were recorded using a surface electromyograph (SEMG). In all vocal tasks, it was observed that women had higher activation (µV) in the suprahyoid and sternocleidomastoid muscles than men, while men had higher activation in the infrahyoid muscles. SEMG is a valid procedure to help define normal vocal characteristics in the studied population, providing reference values during clinical examination. However, it is necessary to adopt a universal system of assessment tasks and standardized measurement techniques to allow for comparisons with future studies.

The high demand for aesthetic treatments among the population has resulted in a wide array of bleaching products available on the market. It is imperative to investigate the potential adverse effects these products may have on dental health. The objective of this systematic review is to assess, based on available experimental in vitro studies in the scientific literature, whether bleaching products exhibit cytotoxic properties against pulp stem cells and fibroblasts. A comprehensive literature search was conducted across the Medline, Scopus, and Lilacs databases using the search formula ((Tooth whitening) OR (bleaching agent)) AND cytotoxicity AND ((stem cell*) OR fibroblast). Following the application of inclusion and exclusion criteria, 14 articles were deemed suitable for full analysis. The most utilized assay in the evaluated studies was the MTT cell viability assay. Fibroblasts emerged as the most scrutinized cell type due to the potential adverse effects of bleaching, such as invasive cervical resorption, which primarily affects the periodontal ligament where fibroblasts are located. It was observed that as the concentration of hydrogen peroxide increases, so does the cytotoxicity of the product. Additionally, other factors such as application time, activation methods, and the type of peroxide used also play a significant role. Bleaching products have been shown to exert cytotoxic effects on fibroblasts. Further exploration of the impact of bleaching agents on dental pulp stem cells is warranted to better understand their implications for these cells.

The aim of this study was to assess the influence of eucalyptol and menthol on the cell viability, migration, and reactive oxygen species production of human gingival fibroblasts (GFs) in vitro. Three different concentrations of eucalyptol and menthol were prepared following ISO 10993-5 guidelines (1, 5, and 10 mM). GFs were isolated from extracted teeth from healthy donors. The following parameters were assessed: cell viability via MTT, Annexin-V-FITC and 7-AAD staining, and IC₅₀ assays; cell migration via horizontal scratch wound assay; and cell oxidative stress via reactive oxygen species assay. Data were analyzed using one-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05. Eucalyptol and Menthol exhibited high cytotoxicity on gingival fibroblasts, as evidenced by cytotoxicity assays. Eucalyptol showed lower levels of cytotoxicity than menthol, compared to the control group. The cytotoxicity of the tested substances increased in a concentration-dependent manner. The same occurred in a time-dependent manner, although even 10 min of exposure to the tested substances showed a high cytotoxicity to the GFs. Commercially available products for oral application with these substances in their composition should be tested for cytotoxicity before their use.

Background: There are four pairs of paranasal sinuses: maxillary, ethmoidal, frontal, and sphenoidal. It is common to see changes in size and shape throughout life, so understanding the effect of age on sinus volume can help in radiographic studies and in planning dental and surgical procedures in the sinus–nasal region. The aim of the present systematic review was to perform a qualitative synthesis of available studies which assess the volumetric characteristics of the sinuses and their changes according to age. Materials and Methods: The present review followed PRISMA 2020 guidelines. A systematic advanced electronic search was performed in five databases (Medline (via PubMed), Scopus, Embase, Cochrane, and Lilacs) in June–July 2022. Studies that assessed the volumetric changes of paranasal sinuses with age were eligible for inclusion. A qualitative synthesis of the methodology and results of the included studies was performed. Quality assessment was performed using the NIH quality assessment tool. Results: A total of 38 studies were included in the qualitative synthesis. Most authors who studied the maxillary and ethmoidal sinuses concluded that it begins its development from birth until the maximum peak of growth, from which it begins to decrease in volume with age. Results regarding the volumetric changes of the frontal and sphenoidal sinuses are mixed. Conclusions: Based on the results of the studies included in the present review, it can be concluded that the volume of the maxillary and ethmoidal sinus appears to decrease with age. Conclusions on the volumetric changes of the sphenoidal and frontal sinuses require further evidence.

The aim of this study was to assess the influence of eucalyptol, chloroform, and Endosolv on the proliferative capability, cell viability, and migration rates of human periodontal ligament stem cells (hPDLSCs) in vitro. Solvent eluates were formulated following ISO 10993-5 guidelines, and 1%, 0.25%, and 0.1% dilutions were prepared. The HPDLSCs were isolated from the extracted third molars of healthy donors. The following parameters were assessed: cell viability via trypan blue and IC50 assays, cell migration via horizontal wound healing assay, cell morphology via cell cytoskeleton staining (phalloidin labeling), and cell oxidative stress via reactive oxygen species assay. The data were analyzed using one-way ANOVA and Tukey’s posthoc tests, and their significance was established at p < 0.05. Chloroform and eucalyptol exhibited significantly higher cytotoxicity on the hPDLSCs in vitro compared to the control group, as shown by the cell viability, migration, morphology, and reactive oxygen species release assays. Alternatively, Endosolv showed adequate cytotoxicity levels comparable to those of the control group. The cytotoxicity of the tested endodontic solvents increased in a dose-dependent manner. The results from the present study highlight the cytotoxicity of chloroform and eucalyptol. Thus, their limited and cautious use is recommended, avoiding solvent extrusion.

(1) Background: The study of the human euphonic voice is a subject that has been researched in recent years from different perspectives. Therefore, it is pertinent to assess the current state of the science. The aim of analyzing the characteristics of normal voice-related publications over the last 11 years is to identify research trends, the numerical and temporal evolution of the publications, their type, and the most-used descriptors. (2) Methods: Bibliometric data from 2011 to 2021 were obtained through several databases. Subsequently, a science mapping analysis was made via VOSviewer software. (3) Results: A total of 901 publications were obtained. The analysis of the scientific production on the field of study regarding the euphonic voice shows a slight increase over the last 11 years, with an average of 82 publications per year. Co-authorship analysis revealed a 6215 authors contributing to the field with a 901 articles (headed by Jiang, J.J. with 18 articles). Keyword co-occurrence analysis highlighted the lack of temporal advancement and variety in the terminology used in the field of voice research. (4) Conclusions: This scientometric study sheds light to the need to broaden in this field of study and the establishment of solid research groups to contribute to its advancement.

Background: At present, there is no clinical consensus on the concept of normal and dysphonic voices. For many years, the establishment of a consensus on the terminology related to normal and pathological voices has been studied, in order to facilitate the communication between professionals in the field of the voice. Aim: systematically review the literature to compare and learn more precisely the measurable and objective characteristics of the acoustic, aerodynamic and surface electromyographic parameters of the normal and dysphonic voices. Methods: The PRISMA 2020 methodology was used as a review protocol together with the PICO procedure to answer the research question through six databases. Results: In total, 467 articles were found. After duplicate records were removed from the selection, the inclusion and exclusion criteria were applied and 19 articles were eligible. A qualitative synthesis of the included studies is presented in terms of their methodology and results. Conclusions: Studying the acoustic, aerodynamic, and electromyographic parameters with more precision, in both normal and dysphonic voices, will allow health professionals working in the field of voice (speech therapy, otorhinolaryngology, phoniatrics, etc.) to establish a diagnostic and detailed consensus of the vocal pathology, enhancing the communication and generalization of results worldwide.