Allyson L. Mellinger
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Allyson L. Mellinger has a total of 3 co-authors. Here are the top co-authors by number of shared publications:
Duy An T. Minior
Division of Neonatal‐Perinatal Medicine East Carolina University/The Brody School of Medicine Greenville North Carolina USA
1 shared publicationKrista McCoy
Department of Biology East Carolina University Greenville North Carolina USA
1 shared publication- Taufika Islam Williams
North Carolina State University
1 shared publication
Latest Publications
- Open Access
- 6 September 2021
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 38 (S1) , e9189
Discovery proteomics of human placental tissue
We describe a label‐free proteomics protocol for the interrogation of the placental proteome. Step‐by‐step directions, including tissue cleanup and preparation, proteolytic digestion, nanoLC–MS/MS data collection and data analysis, are provided. The workflow has been applied toward exploring differential protein expression patterns in placentas from women who have been exposed to drugs during pregnancy relative to those who have not. We collected 20 tissue specimens, each representing a combination of spatially diverse sections across the placenta. These specimens were analyzed in the work described here, to survey information across the entire organ. This protocol can be scaled up or down as needed.
- 6 September 2021
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 38 (S1) , e9189
Open AccessDiscovery proteomics of human placental tissue
We describe a label‐free proteomics protocol for the interrogation of the placental proteome. Step‐by‐step directions, including tissue cleanup and preparation, proteolytic digestion, nanoLC–MS/MS data collection and data analysis, are provided. The workflow has been applied toward exploring differential protein expression patterns in placentas from women who have been exposed to drugs during pregnancy relative to those who have not. We collected 20 tissue specimens, each representing a combination of spatially diverse sections across the placenta. These specimens were analyzed in the work described here, to survey information across the entire organ. This protocol can be scaled up or down as needed.