Yushuang Liu

Outbreaks of infectious diseases pose a major challenge to public health and social development, creating an urgent need for rapid, sensitive, and field-deployable diagnostic platforms. We developed a label-free sensing strategy for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by integrating antibody-functionalized Fe₃O₄@TiO₂@MnO₂ magnetic micromotors with a graphene field-effect transistor (GFET). The micromotors exhibited self-propulsion in H₂O₂ solution due to catalytic oxygen generation, which enhanced target capture and enrichment efficiency. After magnetic separation, the collected micromotor-target complexes were directly analyzed by GFET for quantitative detection. Under optimized conditions, the platform showed a wide linear response and achieved an ultralow limit of detection of ag/mL in PBS. The sensing system also maintained reliable analytical performance in complex matrices, with detection limits of 37.5 ag/mL in human serum and 19.1 ag/mL in soil solution. In addition, the platform exhibited excellent reproducibility and favorable reusability. These results demonstrate that the proposed micromotor-assisted GFET platform provides a sensitive and robust approach for SARS-CoV-2 detection and holds considerable promise for on-site determination of infectious pathogens in complex real-sample environments.

Infectious diseases remain a serious global health threat due to their high transmissibility and mortality, highlighting the urgent need for sensitive and rapid diagnostic tools to enable early detection and effective intervention. Herein, we report an ultrasensitive biosensing platform that integrates Fe₃O₄@UiO-66-NH₂ nanocomposites with a graphene field-effect transistor (GFET) for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The nanocomposites were conjugated with antibodies against SARS-CoV-2, enabling selective capture of viral particles from complex biological matrices. Following magnetic enrichment, the GFET sensor was employed for signal transduction and quantitative analysis. The platform demonstrated a broad dynamic range (1 ag·mL^-1 to 10 ng·mL^-1) with an exceptionally low detection limit of 1.98 ag·mL^-1. Furthermore, successful detection of SARS-CoV-2 antigens in serum samples highlights its potential for point-of-care and real-time diagnostic.

A new detection platform based on CaCO₃-based magnetic micromotor (CaCO₃@Fe₃O₄) integrated with graphene field effect transistor (GFET) was construct and used for on-site SARS-CoV-2 coronavirus pathogen detection. The CaCO₃@Fe₃O₄ micromotor, which was modified with anti-SARS-CoV-2 (labelled antibody, AntiE1), can self-moved in the solution containing hydrochloric acid (HCl) and effective to capture the SARS-CoV-2 coronavirus pathogens. After magnetic field separation, the capture micromotor was detected by GFET, exhibiting a good linear relationship within the range of 1 ag/mL to 100 ng/mL and low detection limit (0.39 ag/mL). Furthermore, the detection platform was also successfully applied to detection of SARS-CoV-2 coronavirus pathogens in soil solution, indicating the potential use in on-site application. Graphical Abstract

Herein, an ultra-sensitive and facile electrochemical biosensor for procalcitonin (PCT) detection was developed based on NiCoP/g-C₃N₄ nanocomposites. Firstly, NiCoP/g-C₃N₄ nanocomposites were synthesized using hydrothermal methods and then functionalized on the electrode surface by π-π stacking. Afterward, the monoclonal antibody that can specifically capture the PCT was successfully linked onto the surface of the nanocomposites with a 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) condensation reaction. Finally, the modified sensor was employed for the electrochemical analysis of PCT using differential Pulse Voltammetry(DPV). Notably, the larger surface area of g-C₃N₄ and the higher electron transfer capacity of NiCoP/g-C₃N₄ endow this sensor with a wider detection range (1 ag/mL to 10 ng/mL) and an ultra-low limit of detection (0.6 ag/mL, S/N = 3). In addition, this strategy was also successfully applied to the detection of PCT in the diluted human serum sample, demonstrating that the developed immunosensors have the potential for application in clinical testing.

Blood glucose monitoring is the premise and basis of diabetes diagnosis and treatment. Previously, we developed polythiophene modified by phenylboronic acid derivatives for non-enzyme detection of glucose and discussed the effect of the structure of phenylboronic acid on the response to glucose. In this study, we investigated the effect of the surface morphology of phenylboronic acid-modified polythiophene (poly(EDOT-PBA)) on glucose-sensing performance. To this end, we developed a fern leaf-like structured poly(EDOT-PBA) film and discussed the effect of surface morphology of poly(EDOT-PBA) for glucose sensing. The modified electrode surfaces were characterized by X-ray photoelectron spectroscopy, scanning electron microscopy, static water contact angle, and impedance measurements. The specific interaction between PBA and glucose was detected by impedancemetry. The concentration-dependent specific response was observed in glucose solution. The sensitivity was 426.1 Ω mM⁻¹ cm⁻² in 0.05–30 mM with a limit of detection of 0.01 mM. The glucose sensor has good selectivity and stability.