Chaowan Guo

Sea rice harbors a diverse array of bioactive constituents, among which the efficient liberation of phytic acid is pivotal for unlocking its full functional potential. In this study, a co-fermentation system integrating yeast and lactic acid bacteria was established to generate a sea rice fermentation filtrate (SRF) with enhanced phytic acid (PA) content, aiming to broaden its application as a cosmeceutical ingredient and to facilitate the high-value utilization of sea rice. Response surface methodology was employed to optimize the inoculation ratios of the two microbial strains and the fermentation duration, and the PA levels were quantified using a commercial assay kit. The anti-melanogenic activity of SRF was assessed in murine B16 melanoma cells, and the underlying molecular mechanisms were elucidated with particular emphasis on the PI3K/AKT/GSK3β/MITF signaling cascade. Under the optimal conditions, specifically Saccharomyces cerevisiae (SC) inoculation at 4.5% and Lactobacillus plantarum (LP) at 6.0% with a fermentation time of 18 h, the PA concentration reached 576.61 μg/mL. Mechanistically, SRF treatment enhanced GSK3β phosphorylation, which was associated with reduced MITF phosphorylation and diminished the expression of downstream melanogenic enzymes, thereby effectively curtailing melanin synthesis, suggesting that SRF, as a complex fermentation product containing multiple bioactive constituents including PA, exhibits potent whitening efficacy. Collectively, these results provide novel perspectives for the valorization of sea rice and the development of natural skin-lightening agents, while also contributing to the extension of the sea rice industrial value chain.

Objective: To evaluate Oligopeptide‐215, a biomimetic peptide engineered from amphibian skin secretions, for its dual capacity to restore skin barrier homeostasis by concurrently targeting physical barrier proteins and inflammatory pathways via JAK–STAT and NF‐κB modulation. Methods: In vitro models used UVB‐damaged HaCaT keratinocytes, LPS‐stimulated RAW264.7 macrophages, and IL‐4/IL‐13‐stimulated HaCaT cells. Assays included cell viability (MTT), migration (scratch), adhesion, barrier proteins (FLG/LOR; ELISA/IF), signaling phosphoproteins (pJAK1/pTYK2/pSTAT3/pSTAT6/pNF‐κB; IF), and inflammatory mediators (NO/PGE₂/TNF‐α/IL‐6/IL‐1β; ELISA). Results: Oligopeptide‐215 antagonizes IL‐13/IL‐4‐mediated JAK–STAT6/STAT3 signaling, thereby restoring expression of critical barrier proteins FLG and LOR. Additionally, it suppresses inflammatory responses by inhibiting NF‐κB‐dependent cytokine production. In UVB‐damaged keratinocytes, Oligopeptide‐215 promotes cellular repair through enhanced proliferation, adhesion, and migration. Conclusion: These findings revealed an innovative repairing mechanism combining physical barrier with immunologic barrier, and established Oligopeptide‐215 as a potential skincare ingredient for skin barrier dysfunction.

Collagen, the most abundant protein in the mammalian extracellular matrix, is critical for skin structure and function, with Type I and Type III collagens being particularly important. Collagen degradation in skin is accelerated by aging and UV exposure, leading to structural and functional impairments. Exogenous collagen supplementation has been shown to restore skin structure and function. Traditional collagen extraction from animal tissues is limited by safety and quality concerns, while recombinant human collagen offers improved safety but faces challenges in solubility and production. This study aimed to construct a chimeric collagen derived from both Type I and Type III collagens and achieve its soluble expression in E. coli. Through translational pausing technology, a recombinant chimeric human collagen containing functional domains of both collagen types was successfully constructed and expressed with a yield of 1.36 g/L. A cysteine-rich C-propeptide domain was fused to enhance assembly and stability. The “dual-function” collagen significantly promoted fibroblast proliferation, migration, and adhesion, while stimulating endogenous Type I and Type III collagen production. Using the C. elegans model, the recombinant protein extended lifespan and enhanced oxidative aging resistance. Skin imaging confirmed its penetration into the dermis, and human skin efficacy tests demonstrated its ability to reduce periorbital wrinkles and crow’s feet. This recombinant “dual” human collagen promotes endogenous collagen synthesis, accelerates skin repair, reduces aging signs, and shows no observed side effects, offering promising potential for anti-aging applications.

This study introduces a novel recombinant humanised collagen (DuCol) developed through codon optimisation and prokaryotic soluble expression, exhibiting exceptional biocompatibility and bioactivity. Structural integrity was confirmed via RP-HPLC, SEM, and CD spectroscopy. In vitro evaluations revealed DuCol’s dose-dependent enhancement of NIH-3T3 fibroblast proliferation, adhesion, and migration. In a D-galactose-induced ageing rat model, subcutaneous implantation of DuCol showcased time-dependent anti-ageing effects. Early-stage intervention (30 days post-injection) markedly upregulated COL1A1 expression through the TGF-β/Smad3 pathway activation, outperforming poly-l-lactic acid (PLLA) in collagen deposition. Histological analysis revealed 23.4% greater dermal thickness in DuCol-treated groups compared to PLLA at 90 days. While PLLA exhibited sustained collagen stimulation beyond 90 days, DuCol exhibited superior early-phase efficacy (p < 0.001) with comparable safety profiles (no inflammatory response observed through 180-day monitoring). The combinatorial PLLA/DuCol (P&C) formulation synergistically enhanced dermal regeneration, achieving a 31.7% thicker collagen matrix than monotherapy groups. These results underscore the potential of DuCol as a novel implantable filler material for skin repair and regeneration.

For the high-value utilization of sake lees (SL), it is essential to explore its potential as a resource for anti-aging bioactives. However, the efficient extraction of SL, the compositional benefits provided, and the resulting anti-aging efficacy in vivo remain to be explored. Thus, a novel continuous phase-transition extraction (CPE) method, an amino acid analyzer, LC-MS, and GC-MS, as well as a classic anti-aging model of Caenorhabditis elegans (C. elegans) were adopted. The results showed that compared to ultrasound-assisted extraction, the total amino acid content of SL extract (SLE) obtained using 80% ethanol in CPE increased by 39.64%, with a notable enhancement in the in vitro scavenging ability of free radicals (p < 0.05). In SLE, the hydrophobic, acidic, and basic amino acids with antioxidant activity accounted for 77.11% of total amino acids. New potential anti-aging compounds were identified, including Lys-Gln, Leu-Arg-Lys, and sphinganine. In particular, 4 mg mL⁻¹ SLE not only promoted a 19.32% increase in the lifespan of C. elegans by enhancing oxidative stress and neuroprotective effects but also ameliorated age-related phenotypes like motoricity and age pigment. Further exploration revealed that the efficacy of SLE is mediated by SKN-1/Nrf2 and HSF-1 pathways, which can be confirmed by the upregulation of key genes, such as skn-1 and hsf-1, especially by inducing a 72.73% increase in nuclear transfer of the transcription factor SKN-1/Nrf2. Taken together, SLE obtained by CPE was abundant in bioactives and contains novel components, thus exerting prominent anti-aging effects in vivo. This study provides a new way to obtain anti-aging active substances efficiently, which is beneficial for application in the fields of health foods and cosmetics.

Morus nigra L. is rich in anthocyanins and other active ingredients, but its extraction residues pose a burden on the environment. In the present study, Morus nigra L. extraction residue resource utilization was achieved through liquid fermentation of Schizophyllum commune, with the aim of enhancing anthocyanin solubilization and evaluating anti-inflammatory activity. Response surface methodology was used to optimize fermentation parameters and quantify anthocyanin fractions by HPLC. The anti-inflammatory effect was evaluated using the lipopolysaccharide-induced inflammation model of human foreskin fibroblast (BJ cell), and the interaction of cyanidin-3-O-glucoside (C3G) with NLRP3, a key target of the pyroptosis pathway, was resolved by molecular docking. Our results indicated that the optimal conditions (substrate 3.4%, inoculum 9%, time 50 h) enabled the total anthocyanin to reach 85.1 μg/mL, of which the C3G content was elevated to 66.7 μg/mL (release efficiency of 83.9%). The fermented filtrate effectively promoted BJ cell proliferation and inhibited the lipopolysaccharide-induced inflammatory response, with the pyroptosis signaling pathway playing a significant role. Molecular docking confirmed that C3G binds strongly to the NLRP3 protein. This technology provides a new strategy for high-value utilization of Morus nigra L. residues and the development of natural anti-inflammatory drugs.

Elastin is a crucial component of the extracellular matrix that plays a significant role in maintaining skin elasticity and firmness. Within the dermis, elastin works in collaboration with collagen to form supportive structures that contribute to the overall health and appearance of the skin. As individuals age, the rate of elastin synthesis gradually declines, resulting in the degradation of skin elastic fibers, which subsequently leads to skin laxity and the formation of wrinkles. Furthermore, environmental factors, particularly ultraviolet radiation, can exacerbate the degradation of elastin and accelerate the aging process of the skin. Elasticity is regarded as a fundamental aspect of skin health; thus, maintaining adequate levels of elastin is essential for delaying the signs of skin aging and preserving the normal physiological functions of the skin. Currently, elastin has become an important ingredient in anti-aging products, demonstrating significant market potential and application prospects. This review focuses on the structural characteristics, physiological functions, and applications of elastin in cosmetics. Additionally, it discusses novel preparation techniques, future development opportunities, and challenges associated with elastin, with the objective of providing a robust theoretical framework for its application and advancement within the cosmetics industry.

Ultraviolet B (UVB) exposure can contribute to photoaging of skin. Cornus officinalis is rich in ursolic acid (UA), which is beneficial to the prevention of photoaging. Because UA is hardly soluble in water, the Cornus officinalis extract (COE) was obtained using water as the antisolvent to separate the components containing UA from the crude extract of Cornus officinalis. The effect of COE on UVB damage was assessed using Caenorhabditis elegans. The results showed that COE could increase the lifespan and enhance the antioxidant enzyme activity of C. elegans exposed to UVB while decreasing the reactive oxygen species (ROS) level. At the same time, COE upregulated the expression of antioxidant-related genes and promoted the migration of SKN-1 to the nucleus. Moreover, COE inhibited the expression of the skn-1 downstream gene and the extension of the lifespan in skn-1 mutants exposed to UVB, indicating that SKN-1 was required for COE to function. Our findings indicate that COE mainly ameliorates the oxidative stress caused by UVB in C. elegans via the SKN-1/Nrf2 pathway.