Peter J. Bechtel
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Peter J. Bechtel has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Ana Cecília Ribeiro de Castro
Embrapa Agroindústria Tropical
1 shared publication - Barry Vant-Hull
Contiguity Consulting, Boulder, CO 80302, USA
1 shared publication - Christopher P. Mattison
Food Processing Sensory Quality, USDA Agricultural Research Service
1 shared publication - Edy Sousa de Brito
Embrapa Agroindústria Tropical
1 shared publication - Heidi J. Chial
BioMed Bridge LLC., 3700 Quebec Street, Suite 100-230, Denver, CO 80207, USA
1 shared publication - Yvette Bren-Mattison
Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
1 shared publication
Latest Publications
- Open Access
- 28 November 2021
- Published by MDPI AG in Antibodies
- Vol. 10 (4) , 46
Characterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars
Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content.
- 28 November 2021
- Published by MDPI AG in Antibodies
- Vol. 10 (4) , 46
Open AccessCharacterization of Anti-Ana o 3 Monoclonal Antibodies and Their Application in Comparing Brazilian Cashew Cultivars
Ana o 3 is an immuno-dominant cashew nut allergen. Four monoclonal antibodies to Ana o 3 (2H5, 6B9C1, 19C9A2, and 5B7F8) were characterized by ELISA and in silico modeling. The 2H5 antibody was the only antibody specific for cashew nut extract. In addition to cashew nut extract, the 6B9C1 and 19C9A2 antibodies recognized pistachio extract, and the 5B7F8 recognized pecan extract. All four antibodies recognized both recombinant Ana o 3.0101 and native Ana o 3. ELISA assays following treatment of purified Ana o 3 with a reducing agent indicated that the 6B9C1 and 19C9A2 antibodies likely recognize conformational epitopes, while the 2H5 and 5B7F8 antibodies likely recognize linear epitopes. In silico modeling predicted distinct epitopes for each of the anti-Ana o 3 antibodies. Screening extracts from 11 Brazilian cashew nut cultivars using all four antibodies showed slight differences in Ana o 3 bindings, demonstrating that these antibodies could identify cultivars with varying allergen content.