Simon Crameri

Summary: Rosewood and palisander (Dalbergia L.f., Fabaceae) are sources of highly valuable tropical timber and include species threatened by habitat degradation and selective logging for national and international trade. Law enforcement depends on reliable and cost-effective species-level identification of timber along the supply chain. The potential of wood anatomy to distinguish between species has not yet been systematically investigated for Dalbergia species from Madagascar. We assessed 36 qualitative and eight quantitative wood anatomical features in 16 Malagasy Dalbergia species that form medium-sized to large trees, representing each species by at least five individuals. We integrated and contrasted the newly collected data with existing data from InsideWood and two previous studies. Principal component analysis of 93 individuals and 29 variables resulted in non-overlapping hulls for eight species with respect to the first two dimensions. Four quantitative features (number of ray tiers per millimetre, number of rays per millimetre, vessel density, and vessel element length) and two qualitative features (scanty paratracheal axial parenchyma and irregular to absent storied structure) were found to be potentially diagnostic to distinguish three single species and three pairs of closely related species. Following our analyses, we provide a provisional microscopic wood anatomical identification key for the 16 Dalbergia species, which can be applied to both logs and sawn wood.

Understanding the genetic changes associated with the evolution of biological diversity is of fundamental interest to molecular ecologists. The assessment of genetic variation at hundreds or thousands of unlinked genetic loci forms a sound basis to address questions ranging from micro- to macroevolutionary timescales, and is now possible thanks to advances in sequencing technology. Major difficulties are associated with (i) the lack of genomic resources for many taxa, especially from tropical biodiversity hotspots; (ii) scaling the numbers of individuals analysed and loci sequenced; and (iii) building tools for reproducible bioinformatic analyses of such data sets. To address these challenges, we developed target capture probes for genomic studies of the highly diverse, pantropically distributed and economically significant rosewoods (Dalbergia spp.), explored the performance of an overlapping probe set for target capture across the legume family (Fabaceae), and built the general purpose bioinformatic pipeline CaptureAl. Phylogenomic analyses of Malagasy Dalbergia species yielded highly resolved and well supported hypotheses of evolutionary relationships. Population genomic analyses identified differences between closely related species and revealed the existence of a potentially new species, suggesting that the diversity of Malagasy Dalbergia species has been underestimated. Analyses at the family level corroborated previous findings by the recovery of monophyletic subfamilies and many well-known clades, as well as high levels of gene tree discordance, especially near the root of the family. The new genomic and bioinformatic resources, including the Fabaceae1005 and Dalbergia2396 probe sets, will hopefully advance systematics and ecological genetics research in legumes, and promote conservation of the highly diverse and endangered Dalbergia rosewoods.

Abstract— The Malagasy rosewood species Dalbergia maritima has a long history of unsustainable exploitation for its beautiful, burgundy-colored heartwood. As currently circumscribed, D. maritima has a wide geographic distribution in eastern Madagascar and exhibits significant morphological, ecological, and genetic variation, suggesting it may comprise more than a single entity. Multivariate analyses of leaf, flower, and inflorescence characters as well as eco-geographic features reveal several morphologically well delimited entities with distinct habitat preferences and/or geographic ranges, which are consistent with results from recent phylogenomic and population genomic studies of Malagasy Dalbergia. Based on these findings, we describe and illustrate two new species from southeastern Madagascar comprising material previously assigned to D. maritima, viz. D. pseudomaritima, characterized by paniculate inflorescences and small, broadly elliptic to orbicular, glabrous leaflets, and D. razakamalalae, distinguished by racemose inflorescences with large flowers, and narrowly ovate to narrowly elliptic, glabrous leaflets. Dalbergia maritima is consequently re-circumscribed to include only populations from east-central Madagascar, within which we recognize two subspecies, D. maritima subsp. maritima, with glabrous leaves, inflorescence axes, and gynoecia, occurring in littoral forest habitats, and D. maritima subsp. pubescens, with indument on these structures, and growing in evergreen humid forest farther inland. Photos are provided for each taxon, along with line drawings for the two new species. Provisional IUCN Red List assessments indicate that all three species are Endangered, D. maritima and D. razakamalalae mainly because of selective logging for trade in their high-quality heartwood, and D. pseudomaritima primarily because of habitat degradation due to land clearing and fire for subsistence agriculture, which has important implications for their conservation and sustainable management.

Understanding the genetic changes associated with the evolution of biological diversity is of fundamental interest to molecular ecologists. The assessment of genetic variation at hundreds or thousands of unlinked genetic loci forms a sound basis to address questions ranging from micro- to macro-evolutionary timescales, and is now possible thanks to advances in sequencing technology. Major difficulties are associated with i) the lack of genomic resources for many taxa, especially from tropical biodiversity hotspots, ii) scaling the numbers of individuals analyzed and loci sequenced, and iii) building tools for reproducible bioinformatic analyses of such datasets. To address these challenges, we developed a set of target capture probes for phylogenomic studies of the highly diverse, pantropically distributed and economically significant rosewoods ( Dalbergia spp.), explored the performance of an overlapping probe set for target capture across the legume family (Fabaceae), and built a general-purpose bioinformatics pipeline. Phylogenomic analyses of Dalbergia species from Madagascar yielded highly resolved and well supported hypotheses of evolutionary relationships. Population genomic analyses identified differences between closely related species and revealed the existence of a potentially new species, suggesting that the diversity of Malagasy Dalbergia species has been underestimated. Analyses at the family level corroborated previous findings by the recovery of monophyletic subfamilies and many well-known clades, as well as high levels of gene tree discordance, especially near the root of the family. The new genomic and bioinformatics resources will hopefully advance systematics and ecological genetics research in legumes, and promote conservation of the highly diverse and endangered Dalbergia rosewoods.

Understanding the genetic changes associated with the evolution of biological diversity is of fundamental interest to molecular ecologists. The assessment of genetic variation at hundreds or thousands of unlinked genetic loci forms a sound basis to address questions ranging from micro-to macro-evolutionary timescales, and is now possible thanks to advances in sequencing technology. Major difficulties are associated with i) the lack of genomic resources for many taxa, especially from tropical biodiversity hotspots, ii) scaling the numbers of individuals analyzed and loci sequenced, and iii) building tools for reproducible bioinformatic analyses of such datasets. To address these challenges, we developed a set of target capture probes for phylogenomic studies of the highly diverse, pantropically distributed and economically significant rosewoods ( Dalbergia spp.), explored the performance of an overlapping probe set for target capture across the legume family (Fabaceae), and built a general-purpose bioinformatics pipeline. Phylogenomic analyses of Dalbergia species from Madagascar yielded highly resolved and well supported hypotheses of evolutionary relationships. Population genomic analyses identified differences between closely related species and revealed the existence of a potentially new species, suggesting that the diversity of Malagasy Dalbergia species has been underestimated. Analyses at the family level corroborated previous findings by the recovery of monophyletic subfamilies and many well-known clades, as well as high levels of gene tree discordance, especially near the root of the family. The new genomic and bioinformatics resources will hopefully advance systematics and ecological genetics research in legumes, and promote conservation of the highly diverse and endangered Dalbergia rosewoods.