Zhengting Yang

Vesicle transport is a fundamental mechanism for intracellular substance transfer and signal transduction, involving the formation, transport, fusion with target membranes, and release of vesicle contents within cells. Issues such as disruption of water balance, difficulty in regulating osmotic pressure, and oxidative stress caused by salt stress can lead to disturbances in the secretion system of plants, thus affecting plant growth and development. Cyclocarya paliurus (C. paliurus), widely used in traditional Chinese medicine, has not been previously reported in terms of how the vesicle transport P24 gene regulates its adaptation to salt stress. In this research project, a total of eight CpP24 genes were successfully identified. Upon examination of gene architecture and conserved sequence elements, the CpP24 genes exhibited a variation in exon count, ranging from 4 to 6. Moreover, the CpP24 gene family’s reaction to salt stress and specific stressors including methyl jasmonate (MeJA), sodium hydrosulfide (NaHS), and sodium nitroprusside (SNP) was further explored in our study. Comprehensive analysis of the expression patterns of CpP24 genes under various conditions showed that salt stress induced the expression of these genes, and the combined treatment of salt stress with specific stresses caused changes in their expression. This study lays a theoretical foundation for further probing into the physiological functions of C. paliurus and the underlying mechanisms regarding its response to environmental stress.

Soil salinity affects approximately 20% of the world’s arable land, presenting a significant challenge for studying the mechanisms by which plants adapt to saline environments. Cyclocarya paliurus, an invaluable research model due to its ecological and medicinal significance, is primarily concentrated in central and southern China. Nevertheless, Cyclocarya paliurus faces challenges from environmental factors such as soil salinization, which adversely impacts its growth, subsequently affecting the yield and quality of its bioactive compounds. The NAC gene family, a critical group of plant-specific transcription factors, plays pivotal roles in responding to abiotic stresses. However, there has not yet been any studies on NAC genes under salt stress in Cyclocarya paliurus. In this study, we identified 132 NAC genes within the Cyclocarya paliurus genome. Our analysis of the conserved structures and gene organization revealed a high degree of conservation in the proteins of the CpNAC gene family. Cis-element analysis unveiled the participation of these genes in a variety of biological processes, including light responses, phytohormone responses, cell cycle responses, and abiotic stress responses. Under salt stress conditions, the expression of 35 CpNAC genes changed significantly, indicating a response to salt treatment. Furthermore, we provided additional evidence for the identification of the NAC gene family and revealed their potential positive regulatory role in signal transduction by conducting a transcriptional activation activity analysis of CpNAC132(D) and CpNAC040, which are homologous to Arabidopsis thaliana NAC062/91 and NAC103, respectively. This research not only advances our comprehension of the salt stress adaptation in Cyclocarya paliurus but also provides robust support for future investigations into plant responses to environmental stress and the cultivation of salt-tolerant crops.

Environmental stresses can disrupt protein structure, resulting in unfolded or misfolded proteins, thereby triggering endoplasmic reticulum (ER) stress. The unfolded protein response (UPR), particularly as activated by Arabidopsis AtbZIP60 gene, is pivotal for counteracting ER stress and ensuring cell survival. The medicinal plant, Cyclocarya paliurus, known for its wealth of beneficial compounds, is threatened by environmental stresses, limiting the exploration of its therapeutic potential. In order to better exploit and utilize its value, it is necessary to understand the signal pathway of environmental stresses. Here, we identify a homolog of AtbZIP60 in C. paliurus, termed CpbZIP11, which can be upregulated by tunicamycin. The conserved double stem-loop structure in its mRNA is spliced under environmental stresses. This splicing event results in a novel CpbZIP11 mRNA variant, leading to the production of a nuclear-localized CpbZIP11 protein with transcriptional activation activity in yeast. We further delve into the study of evolutionary lineage and motif conservation of CpbZIP11 homologs across various plant groups. This research illuminates the stress adaptation mechanisms in C. paliurus and deepens our understanding of the bZIP evolution, which endows versatility for the understanding of this transcription factor.

WRKY proteins are key transcription factors involved in a range of physiological processes and environmental adaptations. Yet, little is known about the role WRKY genes play in Cyclocarya paliurus (Batal.) Iljinskaja (C. paliurus) a significant medicinal plant belonging to the Juglandaceae family. Leveraging the recently released whole-genome sequence of C. paliurus, we conducted an in-depth, genome-wide study on the expression of WRKY genes under environmental stresses. We identified 80 CpWRKY genes, classifying them into distinct groups and subgroups based on their structural and phylogenetic characteristics. Synteny analysis and phylogenetic comparison provided insights into the evolutionary traits of these genes. We further investigated the response of the WRKY gene family to salinity stress in combination with the following specific stressors: Methyl jasmonate (MeJA), sodium hydrosulfide (NaHS), and sodium nitroprusside (SNP), which induce the production of signaling molecules jasmonates (JA), hydrogen sulfide (H₂S), and nitric oxide (NO), respectively. Our comprehensive analysis of CpWRKY gene expression patterns under various conditions revealed that combined treatments were more effective in inducing up-regulation of CpWRKY gene expression than individual treatments.

For the purpose of improving the classification accuracy of single-trial electroencephalogram (EEG) signal during motor imagery (MI) process, this study proposed a classification method which combines intrinsic mode functions (IMFs) energy entropy and improved empirical mode decomposition (EMD) scheme. Singular value decomposition (SVD), Gaussian mixture model (GMM), EMD and IMF energy entropy were employed for the newly designed scheme. After removing noise and artifacts from acquired EEG signals in EEGLAB, SVD was applied, and the singular values were clustered by GMM. The insignificant characteristics indicated by the small SVD values were then removed, and the signals were reconstructed, feeding to EMD algorithm. Those IMFs mapping to [ ], [ ], [ ] and [ ] frequencies were selected as the major features of the Electroencephalogram signal. The SVM classifier with Radial Basis Function Neural Network, linear, and polynomial kernel functions and voting mechanism then kicked in for classification. The results were compared with that of the traditional EMD and EEMD through simulation, showing that the proposed scheme can eliminate mode mixing effectively and improve the single-trial EEG signal classification accuracy significantly, suggesting the probability of designing a more efficient EEG control system based on the proposed scheme.