Emmanuel Velidakis

During the COVID-19 pandemic, wide use of 3D printing technologies has been enabled. Fused filament fabrication (FFF) is the most widely used technique in 3D printing communities worldwide for the fabrication of medical components such as face shields and respiratory valves. In the current study, the potential of Polyamide 12 (PA12) silver-doped antibacterial nanopowder (AgDANP) nanocomposites is evaluated for everyday FFF usage. Filling loadings of 1.0-2.0-3.0 and 4.0 wt.% were selected for nanocomposite preparation. Mechanical performance analysis was conducted on the basis of tensile, flexural, impact, and Vickers microhardness measurements in FFF 3D-printed specimens. Scanning Electron Microscopy (SEM) images were used for morphology and processing evaluation, as well as thermal performance measurements, conducted by Thermogravimetric Analysis (TGA) tests. Finally, the antibacterial performance was tested using the agar-well diffusion screening method, and the shape effect of the specimens was also investigated. The addition of 2.0 wt.% AgDANPs resulted in an enhancement of approximately 27% for both tensile and flexural stresses, while the antibacterial performance was sufficiently high among the nanocomposites tested. The shape effect exhibited the potential for antibacterial performance at low filling ratios, while the effect was diminished with increasing filler of AgDANPs.

In this study, Cuprous Oxide (Cu₂O), known for its mechanism against bacteria, was used as filler to induce biocidal properties on a common commercial resin stereolithography (SLA) 3D printing resin. The aim was to develop nanocomposites suitable for the SLA process with a low-cost process that mimic host defense peptides (HDPs). Such materials have a huge economic and societal influence on the global technological war on illness and exploiting 3D printing characteristics is an additional asset for these materials. Their mechanical performance was also investigated with tensile, flexural, Charpy’s impact, and Vickers microhardness tests. Morphological analysis was performed through scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy-dispersive X-ray spectroscopy (EDS) analysis, while the thermal behavior was studied through Thermogravimetric Analysis (TGA). The antibacterial activity of the fabricated nanocomposites was investigated using a screening agar well diffusion method, for a gram-negative and a gram-positive bacterium. Three-dimensional printed nanocomposites exhibited antibacterial performance in all loadings studied, while their mechanical enhancement was approximately 20% even at low filler loadings, revealing a multi-functional performance and a potential of Cuprous Oxide implementation in SLA resin matrices for engineering and medical applications.

Metals, such as silver, gold, and copper are known for their biocidal properties, mimicking the host defense peptides (HDPs) of the immune system. Developing materials with such properties has great importance in medicine, especially when combined with 3D printing technology, which is an additional asset for various applications. In this work, copper nanoparticles were used as filler in stereolithography (SLA) ultraviolet (UV) cured commercial resin to induce such biocidal properties in the material. The nanocomposites developed featured enhanced mechanical responses when compared with the neat material. The prepared nanocomposites were employed to manufacture specimens with the SLA process, to be tested for their mechanical response according to international standards. The process followed was evaluated with Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), energy-dispersive X-ray spectroscopy (EDS), and thermogravimetric analysis (TGA). The antibacterial activity of the fabricated nanocomposites was evaluated using the agar-well diffusion method. Results showed enhanced mechanical performance of approximately 33.7% in the tensile tests for the nanocomposites filled with 1.0 wt.%. ratios, when compared to the neat matrix material, while this loading showed sufficient antibacterial performance when compared to lower filler loadings, providing an added value for the fabrication of effective nanocomposites in medical applications with the SLA process.