Chollada Sodarat

Erythrocyte osmotic fragility is an excellent parameter for evaluating the red blood cell (RBC) membrane, which may be abnormal in several pathological conditions. The flow cytometer is a powerful tool that analyzes a single cell in a solution and can detect alterations in RBCs, providing key differential diagnostic information. Both the osmotic fragility test (OFT) and flow cytometry are valuable diagnostic tools in veterinary medicine, but their diagnostic usefulness in anemic cats has not yet been determined. This study aimed to evaluate RBC membrane strength using an OFT in non-anemic and anemic cats and to compare RBC size and density using a flow cytometer in non-anemic and anemic cats. A total of 18 cats in the non-anemic group and 18 cats in the anemic group, divided into adults and seniors, were included in this study. Blood samples were collected for a complete blood count (CBC) and blood chemistry. The remaining blood was used for OFT to evaluate 50% hemolysis from the hemolysis curve and for the flow cytometer to measure forward scatter characteristics (FSC) and side scatter characteristics (SSC). The result of OFT showed no significant difference in OF between normal and anemic cats in the adult and senior groups. In terms of flow cytometry analysis, normal and anemic cats in the adult group showed no significant difference in the FSC (p = 0.769). On the other hand, the FSCs of normal senior cats were significantly higher than those of anemic cats (p = 0.0486). The SSCs of normal cats were significantly higher than those of anemic cats in the adult group (p = 0.048). However, the SSCs of the senior group showed no significant difference (p = 0.074). Based on these results, we concluded that, in the senior group, normal cats had higher FSCs than anemic cats, and in the adult group, normal cats had higher SSCs than anemic cats. However, both normal and anemic cats exhibited similar osmotic fragility. Further studies on various diseases are suggested.

The objective of this study was to investigate the therapeutic effects of inactivated Propionibacterium acnes and lipopolysaccharide derived from Escherichia coli cells in cats affected by feline panleukopenia virus (FPV). A retrospective study of 80 FPV-positive cats was divided into two groups: a treatment group receiving inactivated Propionibacterium acnes and lipopolysaccharide derived from Escherichia coli cells along with supportive treatment and a no-treatment group receiving only supportive treatment. There was no significant difference in the total white blood cell counts between the two groups. However, the total white blood cell counts of both groups were low on day 0 and increased significantly on days 3 and 6 of treatment. Additionally, the white blood cell counts in the treatment group significantly increased during days 3 to 6 compared with those of the no-treatment group (p < 0.01). The mortality rate was not significantly different between the two groups. In a prospective study, the serum and fecal immunoglobulin A (IgA) levels were measured in both groups. There were no significant differences in IgA levels between the two groups in either the serum or feces.

Dermatophytosis is a disease caused by dermatophytes, a group of fungi that can cause disease both in humans and animals. The important genera that are pathogenic in animals include Trichophyton and Microsporum. Microsporum canis is an important species because it can cause zoonosis and is commonly found in domestic animals. Cats, which live very close to humans, may expose humans to this pathogen. This research focused on the epidemiology of M. canis found in cats. Hair samples were collected via the Mackenzie technique from cats with and without skin lesions, preliminarily examined with 10% KOH preparation, and cultured for fungal identification. Samples were confirmed with molecular techniques including polymerase chain reaction, gel electrophoresis, and sequencing. Samples were collected from 138 cats located in 93 households, 43 from cats with skin lesions (31.16%) and 95 from cats without skin lesions (68.84%). Eighteen cats with lesions (13.04%) and ten cats without lesions (7.2%) were found to carry M. canis. In eleven of the eighteen cats both with skin lesions and positive for M. canis (61.11%), the pathogen was found both at the site of the lesion and at other sites in the body. Because the pathogen can be found in the hair of cats with and without skin lesions, owners, keepers, veterinarians, and others who come into contact with these animals are at risk of infection if they are not aware or do not take precautions after contact with them.