Mateus Edson da Silva

Objetivo: avaliar o envolvimento do polimorfismo de nucleotídeo único (SNP) rs670 no tamanho das partículas e na ação antioxidante do HDL. Métodos: foram coletados dados socioeconômicos, sociodemográficos e clínicos. Além disso, foram determinados dados antropométricos, perfis glicêmicos e lipídicos, níveis de apolipoproteína A-I e B, níveis de ácidos graxos não esterificados, índice ômega-3, genotipagem do SNP rs670 e subfrações de partículas de HDL e função antioxidante. Resultados: 78 indivíduos, com prevalência de mulheres (46, 59%), idade média de 40 (±14) anos, presença de fatores de risco cardiovascular (n = 72; 92,3%), entre outros. De acordo com o SNP rs670, não foi observada diferença entre os parâmetros sociodemográficos, glicolipídicos, antropométricos e capacidade antioxidante da HDL. No entanto, o genótipo CC mostrou correlações entre o colesterol HDL e a glicemia(rho=-0,304), triglicerídeos (rho=-0,324), índice de massa corporal (rho=-0,439) e circunferência da cintura (rho=-0,516). Os genótipos CT e TT mostraram correlações entre a relação HDL 1-2/9-10 e a glicemia (rho=-0,576) e a relação Apo B/Apo A-I (rho=-0,629). Além disso, a presença do alelo T pode ser prevista pela relação HDL 1-2/9-10. Conclusões: conclui-se que o alelo T polimórfico estava associado a relações HDL mais elevadas em relação às mais baixas, possivelmente afetando sua funcionalidade.

Aim: To evaluate the urinary biomarkers related to sepsis in preterm newborns (NBs) and to investigate the predictive capacity of these biomarkers for a longer hospital stay.Methods: Serum and urine were collected from 27 healthy NBs, 24 NBs with neonatal infection without sepsis and 11 NBs with sepsis for the measurement of sindecan-1, lipocalin associated with urinary neutrophil gelatinase (uNGAL), urinary cystatin-C (uCysC) and urinary kidney injury molecule-1.Results: Levels of uNGAL and urinary cystatin-C were elevated in NBs with sepsis and neonatal infection, and uNGAL was significant predictor of hospital stay longer than 30 days (odds ratio: 1.052; 95% CI: 1.012-1.093; p = 0.01).Conclusion: uNGAL was associated with sepsis in preterm NBs and was useful to predict extended hospital stay.

Diabetes mellitus (DM) is a major cause of chronic kidney disease (CKD) and changes in lipoprotein. Literature data suggest that cholesterol associated with high-density lipoprotein (HDLc) plays a protective role against albuminuria and diabetic nephropathy. These factors are related with changes in serum apolipoproteins such Apo A-I. This work aims to evaluate the correlation of apolipoprotein A-I with renal function in diabetic outpatients. Samples were collected from 281 outpatients for analyses of glycated hemoglobin (HbA1c), serum creatinine (SCr), urea (BUN), cholesterol (TC), triglycerides (TG), HDL-cholesterol (HDLc) and estimation of glomerular filtration rate (eGFR). Urinary samples were obtained to assess urine albumin creatinine ratio (UACR). Triglyceride-glucose index (TyG) was calculated to estimate insulin resistance. The mean age was 61,5 ± 16,4 years. Most patients were female, Afro-Mestizo and over 50 years of age. Pearson's revealed negative correlation of creatinine with HDLc and Apo A-I. There was a correlation of eGFR with HbA1c, TG and HDLc. TyG presented a negative correlation with Apo A-I and HDLc. The highest quartiles of UACR presented the highest TyG and the lowest Apo A-I. In conclusion, Apo A-I indicated an association with insulin resistance and changes in renal function parameters, especially as a factor associated with the onset of albuminuria, with a better predictive potential than the HDLc.