Xiaolin Huang

The rabbitfish, Siganus oramin, is a commercially important table fish in southeastern China. However, there have been few studies on its gonad development and reproduction regulation. Comparative transcriptome analysis was first performed on adult male and female gonads of S. oramin. In total, 47,070 unigenes were successfully assembled and 22,737 unigenes were successfully annotated. Through comparative transcriptome analysis of male and female gonads, a total of 6722 differentially expressed genes were successfully identified, with 3528 upregulated genes and 3154 downregulated genes in the testes. In addition, 39 differentially expressed reproduction-related genes were identified. Finally, quantitative real-time PCR was used to validate the expression levels of several differentially expressed genes. These results provide important data for further studying the function of reproduction-related genes and the molecular mechanism regulating gonad development and reproduction in S. oramin.

Six isonitrongenous and isolipidic diets were formulated to feed 360 fish (mean initial body weight: g) with graded levels of sodium acetate (SA1 (0), SA2 (250 mg/kg), SA3 (500 mg/kg), SA4 (1000 mg/kg), SA5 (2000 mg/kg), and SA6 (4000 mg/kg)) for 8 weeks. The results revealed that weight gain rate, specific growth rate, and feed intake of golden pompano were significantly increased in SA3 and SA4 treatments ( ). Dietary SA level significantly improved lipid contents and gumminess of muscle ( ). Supplement levels of dietary SA significantly influenced plasma biochemical parameters containing triglyceride, glucose, high-density lipoprotein cholesterol, complements, and immunoglobulin M ( ). Appropriate supplement levels of dietary SA significantly increased enzyme activities of hepatic superoxide dismutase and total antioxidant capacity and decreased malondialdehyde contents ( ). Furthermore, appropriate SA levels downregulated the expression levels of Keap1, IL-8, TNF-α, and NF-κB genes and upregulated the expression levels of Nrf2 and TGF-β genes of the spleen ( ). Excess SA caused negative effects for Trachinotus ovatus. The optimum supplement level of SA for juvenile golden pompano was evaluated to be 1423.67 mg/kg based on WGR.

INPLASY202190097 Association between Lipocalin-2 levels and Gestational Diabetes Mellitus: A Systematic Review and Meta-Analysis Keywords: Gestational diabetes mellitus; lipocalin-2; association. D…