Nguyen Thanh Triet

Trà Longi (Camellia longii) là loài thực vật đặc hữu của Việt Nam nhưng vẫn chưa được nghiên cứu đầy đủ về tiềm năng sinh học. Nghiên cứu này nhằm đánh giá hàm lượng polyphenol (TPC), flavonoid toàn phần (TFC), hoạt tính chống oxy hóa và khả năng ức chế α-glucosidase của các cao chiết từ lá và hoa của Trà Longi. Kết quả cho thấy TPC dao động từ 324,89 ± 6,74 đến 482,00 ± 8,82 mg GAE/g cao chiết, trong khi TFC đạt 42,31 ± 0,68 đến 48,07 ± 1,80 mg QE/g cao chiết. Các cao chiết thể hiện hoạt tính chống oxy hóa mạnh, với giá trị IC50 trong thử nghiệm DPPH từ 13,20 ± 0,35 đến 17,91 ± 0,22 µg/mL và trong thử nghiệm ABTS từ 54,12 ± 0,77 đến 60,80 ± 1,74 µg/mL. Đồng thời, các cao chiết cũng ức chế mạnh α-glucosidase (IC₅₀ = 29,80 ± 0,15–37,99 ± 0,24 µg/mL). Những kết quả này cung cấp cơ sở khoa học ban đầu cho việc khai thác Trà Longi như một nguồn nguyên liệu tiềm năng trong phát triển các sản phẩm hỗ trợ chống oxy hóa và kiểm soát glucose huyết.

This study was conducted to evaluate the anti-hyperuricemic effect of the 70% ethanol extract from a traditional formula containing Siegesbeckia orientalis, Xanthium strumarium, and Smilax glabra (HKT extract). The extract was screened for in vitro xanthine oxidase (XO) inhibitory activity to determine its biological effects. The anti-hyperuricemic effect in mice was then determined using a model of acute hyperuricemia induced by the intraperitoneal injection of potassium oxonate. The in vitro study showed that the combined extract possessed the potential XO inhibitory activity with the IC50 value of 28.99 μg/mL. The in vivo study demonstrated that the ethanol extract at the doses of 0.588 g/kg and 1.177g/kg reduced uric acid levels at the inhibitory percentage by 31.40% and 30.03%, respectively, compared to the pathological control group. These findings suggest the therapeutic potential of the HKT extract against hyperuricemia.

Objectives: This study aims to develop and validate a reliable procedure for the determination of cadmium, lead, arsenic, and mercury in herbal medicines using atomic absorption spectroscopy. The method was designed to achieve complete mineralization, minimize analyte loss, and reduce analysis time while ensuring accurate quantification of heavy metals in complex matrices. Given the potential toxicity of heavy metals, ensuring accurate quantification is crucial for quality control and regulatory compliance. Methods: A microwave-assisted digestion technique using 65% nitric acid was employed for sample preparation. The analytical method was optimized using Design Expert 12 software to maximize recovery efficiency. Linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) were validated according to the ICH Q2(R1) guideline. Results: Optimization results indicated that the recovery rates for Cd, Pb, As, and Hg were all approximately 90%, confirming the method's robustness and suitability for accurate heavy metal quantification in herbal medicine products. The method demonstrated great linearity (r > 0.995), with limits of detection (LOD) of 0.1-10 ng/ml and limits of quantification (LOQ) of 0.3-33.3 ng/ml. The relative standard deviation (RSD) was below 5%, confirming precision. The validated procedure was successfully applied to herbal medicine products. Conclusion: The developed procedure provides a robust, accurate, and validated approach for the quantification of heavy metals in herbal medicines. Its successful application to real samples highlights its potential for routine quality control and regulatory assessment.

Trevesia Vis. species are small evergreen trees belonging to the family Araliaceae that occur naturally throughout Asia. The multiple parts of Trevesia species have been used in traditional medicinal systems in several countries for their potential therapeutic properties, including pain relief, inflammation, fevers, microbial infections, diabetes, cancer, edema, bone fractures, and arthritis. This work presents a fascinating exploration of previous studies on Trevesia species, especially T. palmata , delving into their phytochemical constituents, biological activities, and toxicology, inviting you to uncover their potential further. This paper database meticulously compiles valuable data gathered from all accessible literature between 1842 and 2024 through a wide range of resources, including Web of Science, Scopus, Google Scholar, PubMed, Science Direct, Springer, and other scientific databases, ensuring a comprehensive and reliable overview of the research on Trevesia species. Various bioactive phytochemicals, such as triterpenoid saponins, flavonoids, and phenolic compounds, have been found in multiple parts of these plants. Chemotaxonomic investigations have revealed that the genus Trevesia may be closely related to the genera Aralia , Acanthopanax , and Hedera . Pharmacological studies showed that extracts and phytocompounds from T. palmata exhibit various biological activities, such as antiproliferative, anticancer, antibacterial, antifungal, antiviral, antioxidant, anti-inflammatory, antidiabetic, analgesic, thrombolytic, and hepatoprotective activities. Moreover, numerous toxicological reports revealed the safety of the T. palmata plant. For the first time, this paper highlights the role of therapeutic effects and health benefits of Trevesia species in food and medicine while also providing an overview of the current study status and future prospects for Trevesia species, mainly T. palmata .

Background: Conamomum vietnamense is a new species discovered in Vietnam with important pharmacological potential. Questions: What are the major phytochemical constituents of C. vietnamense rhizomes? Does the n-hexane fraction have cytotoxic effects against five human cancer cell lines (MCF-7, SK-LU-1, HeLa, MKN-7, and HL-60)? Studied species: Conamomum vietnamense N.S.Lý & T.S.Hoang (Zingiberaceae) Study site and dates: Loc Bac, Bao Lam, Lam Dong, Vietnam, 2022-2023 Methods: Volatile components and secondary metabolite groups were identified using gas chromatography-mass spectrometry analysis and color/precipitation reactions, respectively. Rhizome n-hexane fractionated extract of C. vietnamense was tested against cancer cell lines in vitro and in silico. Results: Twenty-three compounds were identified in the rhizome fraction of C. vietnamense, where in α-eudesmol (26.84 %), β-eudesmol (15.02 %), cryptomeridiol (14.36 %), γ-eudesmol (6.21 %), eucalyptol (4.38 %), and eudesm-7(11)-en-4-ol (3.11 %) were proved as major compounds. This n-hexane fractionated extract showed a cytotoxic effect against five human cancer cell lines, namely MCF-7, SK-LU-1, HeLa, MKN-7, and HL-60, with IC50 values varying from 59.04 to 172.43 µg/mL. Along with the in vitro activity test, the docking study demonstrated that α-eudesmol, guaiol, and nerolidol showed the most potential binding affinities to human PTPN2 with binding energies of -29.71, -29.29, and -28.87 kcal/mol, respectively. Furthermore, β-eudesmol, guaiol, and cryptomeridiol exhibited the strongest affinity for the binding site with human IGF-1R kinase with docking scores of -29.29, -28.87, and -32.64 kcal/mol. Conclusions: The current results implied that C. vietnamense rhizomes and its dominant components could be a source of therapeutic interest for cancer.

Alpinia pinnanensis T.L.Wu & S.J.Chen, a species in the Zingiberaceae family, was identified and described from Phu Tho Province, Vietnam. In this study, the essential oil (EO) was extracted from A. pinnanensis rhizomes using hydrodistillation, and its phytochemical constituent was analyzed by gas chromatography-mass spectrometry (GC-MS). The main ingredients identified were β-myrcene (18.72%), farnesol (12.17%), β-linalool (11.91%), and 1,8-cineole (8.82%). The EO exhibited weak antimicrobial activity against Bacillus cereus, Pseudomonas aeruginosa, and Candida albicans with minimum inhibitory concentration (MIC) values ranging from 128 to 256 µg/mL, comparable to positive controls, streptomycin and cycloheximide. Additionally, the EO exhibited cytotoxic effects against HeLa (human cervical carcinoma) and HepG2 (human hepatocellular carcinoma) cell lines, with IC50 values of 8.50 ± 0.29 µg/mL and 7.84 ± 0.15 µg/mL, respectively. This is the first study to report on the phytochemical composition, antimicrobial, and cytotoxic effects of the EO from A. pinnanensis rhizomes.