Xia Lei
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Xia Lei has a total of 19 co-authors. Here are the top co-authors by number of shared publications:
Chenda Yue
Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing, China
1 shared publication- Chenda Yue
Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing 400044, China
1 shared publication - Jianping Xie
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing, China
1 shared publication Jinyi Chen
Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing, China
1 shared publication- Julia Li Zhong
Key Laboratory of Biorheological Science and Technology of Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China
1 shared publication - Junqi Xu
School of Life Sciences, Southwest University, Chongqing 400715, China
1 shared publication
Latest Publications
- 14 March 2022
- Published by Elsevier in Photodiagnosis and Photodynamic Therapy
- Vol. 38, 102802
In vitro study of the effect of ALA-PDT on Mycobacterium abscessus and its antibiotic susceptibility
- 14 March 2022
- Published by Elsevier in Photodiagnosis and Photodynamic Therapy
- Vol. 38, 102802
In vitro study of the effect of ALA-PDT on Mycobacterium abscessus and its antibiotic susceptibility
- Open Access
- 14 March 2022
- Published by MDPI AG in Antioxidants
- Vol. 11 (3) , 546
ALA_PDT Promotes Ferroptosis-Like Death of Mycobacterium abscessus and Antibiotic Sterilization via Oxidative Stress
Mycobacterium abscessus is one of the common clinical non-tuberculous mycobacteria (NTM) that can cause severe skin infection. 5-Aminolevulinic acid photodynamic therapy (ALA_PDT) is an emerging effective antimicrobial treatment. To explore whether ALA_PDT can be used to treat M. abscessus infections, we conducted a series of experiments in vitro. We found that ALA_PDT can kill M. abscesses. Mechanistically, we found that ALA_PDT promoted ferroptosis-like death of M. abscesses, and the ROS scavenger N-Acetyl-L-cysteine (NAC) and ferroptosis inhibitor Ferrostatin-1 (Fer-1) can mitigate the ALA_PDT-mediated sterilization. Furthermore, ALA_PDT significantly up-regulated the transcription of heme oxygenase MAB_4773, increased the intracellular Fe2+ concentration and altered the transcription of M. abscessus iron metabolism genes. ALA_PDT disrupted the integrity of the cell membrane and enhanced the permeability of the cell membrane, as evidenced by the boosted sterilization effect of antibiotics. In summary, ALA_PDT can kill M. abscesses via promoting the ferroptosis-like death and antibiotic sterilization through oxidative stress by changing iron metabolism. The study provided new mechanistic insights into the clinical efficacy of ALA_PDT against M. abscessus.
- 14 March 2022
- Published by MDPI AG in Antioxidants
- Vol. 11 (3) , 546
Open AccessALA_PDT Promotes Ferroptosis-Like Death of Mycobacterium abscessus and Antibiotic Sterilization via Oxidative Stress
Mycobacterium abscessus is one of the common clinical non-tuberculous mycobacteria (NTM) that can cause severe skin infection. 5-Aminolevulinic acid photodynamic therapy (ALA_PDT) is an emerging effective antimicrobial treatment. To explore whether ALA_PDT can be used to treat M. abscessus infections, we conducted a series of experiments in vitro. We found that ALA_PDT can kill M. abscesses. Mechanistically, we found that ALA_PDT promoted ferroptosis-like death of M. abscesses, and the ROS scavenger N-Acetyl-L-cysteine (NAC) and ferroptosis inhibitor Ferrostatin-1 (Fer-1) can mitigate the ALA_PDT-mediated sterilization. Furthermore, ALA_PDT significantly up-regulated the transcription of heme oxygenase MAB_4773, increased the intracellular Fe2+ concentration and altered the transcription of M. abscessus iron metabolism genes. ALA_PDT disrupted the integrity of the cell membrane and enhanced the permeability of the cell membrane, as evidenced by the boosted sterilization effect of antibiotics. In summary, ALA_PDT can kill M. abscesses via promoting the ferroptosis-like death and antibiotic sterilization through oxidative stress by changing iron metabolism. The study provided new mechanistic insights into the clinical efficacy of ALA_PDT against M. abscessus.