Giuseppe Ianiri

Wild edible plants (WEPs) are increasingly recognized as valuable components of Mediterranean food systems; however, reliable compositional data remain scarce for many traditionally consumed species, particularly for micronutrients whose concentrations are influenced by collection site and harvesting year. Information on B-group vitamins in the leaves of commonly consumed WEPs remains particularly limited. This study characterized the carotenoid, tocol, thiamine and riboflavin profiles and amounts in the leaves of Malva sylvestris L. and Foeniculum vulgare Mill., two representative Mediterranean WEPs. Plants were collected using a replicated multi-site sampling strategy over two consecutive harvesting years to improve the representativeness of the compositional dataset and assess natural variability. To our knowledge, this is the first study reporting thiamine and riboflavin concentrations in the leaves of both species using validated chromatographic methods. The compound concentrations differed significantly according to species, harvesting year and collection site, highlighting the natural variability of nutrient composition. Maximum values reached 2233 mg Retinol Equivalents (RE)/100 g for vitamin A activity, 8.9 mg Tocopherol Equivalents (TE)/100 g for vitamin E activity, and approximately 8 mg/100 g for thiamine. According to the criteria of EU Regulation No. 1169/2011, a 100 g portion of fresh leaves would qualifiy these species as sources of vitamins A, E, and B1, while their riboflavin content also contributes to dietary vitamin B2 intake. These findings provide robust compositional data for two representative Mediterranean WEPs, supporting food composition databases, dietary assessment and the valorization of wild edible plants as sustainable, functional food ingredients.

The measurement of atmospheric deposition fluxes is an excellent tool for assessing the contamination of territory and the subsequent exposure of the population to major contaminants through the food chain. In this context, the aim of this study was to measure the polycyclic aromatic hydrocarbon (PAH) deposition fluxes in the city of Rome (ISS Station) during the year 2022/2023 at two different heights above the ground (vertical profile), in order to evaluate the influence that the vertical profile has on PAH deposition. Two measuring positions were identified, one at street level and one at a height of 20 m. The collection of bulk atmospheric depositions was carried out approximately every 30 days, and the PAHs were determined according to the indications given in ISTISAN Report 06/38 and Standard UNI EN 15980:2011. The results show that throughout the year, the deposition rates of settleable dust were always higher at the lower (annual average of 48.5 mg m⁻² day⁻¹) collection position than at the higher position (annual average of 17.5 mg m⁻² day⁻¹). Despite this difference, the concentrations and profiles of the main PAHs analyzed, as indicated in EU Directive 2024/2881, in the dust collected at the two positions were almost similar, showing that the vertical profile did not influence the composition and concentration of PAHs in the collected settleable dust. Furthermore, a comparison of the deposition rates of sedimentable dust and PAHs with the legislative references currently present in Europe was made, highlighting that in the city of Rome during the monitoring period of this study, the values of dust and PAHs were lower than the limit and guide values and were also in line with other Italian urban locations.

Plant leaves can be used to determine the atmospheric deposition of organic contaminants, including polycyclic aromatic hydrocarbons (PAHs), to assess the contamination status of an area. The purpose of this study was to develop an analytical method for the determination of PAHs deriving from atmospheric deposition using Malva sylvestris leaves. Analytes were recovered from the leaves of the plant using cyclohexane as an organic solvent and subsequent sonication. The percentage recoveries (R%) were good (from 65.8 ± 3.2 to 104.2 ± 16.9), together with the instrumental analytical parameters, including correlation coefficients (r) ≥ 0.995 for all PAHs. The instrumental analysis was carried out using GC-MS in total ion current and single ion monitoring at the same time. Real samples taken from urban environments have shown that they are not always the most contaminated. At the Palermo site, leaves were observed to have high amounts of PAHs due to the deposition of dust generated by combustion processes that occurred near the sampling site. Further studies are recommended to compare the use of plants and classical sampling systems for monitoring the atmospheric deposition of key contaminants toxic to human health.

The determination of total atmospheric deposition (bulk) is an essential tool to assess the state of environmental contamination and the consequent exposure of the population to persistent organic pollutants (POPs) through the intake of contaminated food. Over the past 20 years, international authorities and the European Union through various pieces of legislation have emphasised the importance of conducting monitoring and studies on depositions to better understand their impact on the environment and human health without setting reference values. Despite the absence of such values, several European countries, through national laws, have adopted limit values and/or guideline values for the deposition fluxes of some organic (dioxins, furans, polychlorinated biphenyls and polycyclic aromatic hydrocarbons) and inorganic persistent pollutants (metals). The aim of this review is both to summarise the present European legislation on depositions both to discuss the different legislations adopted by the various member states. Furthermore, a focus of this paper will be dedicated to the Italian legislation, where there is currently no specific guideline values for POPs in atmospheric deposition. In any case, some national authorities in Italy, such as the National Institute of Health (ISS) and the Regional Environmental Protection Agencies (ARPA), have conducted numerous monitoring activities on depositions, providing the scientific community and policymakers with numerous data on which to establish national reference values.

The aim of this study was to determine the profile and contents of carotenoids, tocols and B1 and B2 vitamins in different parts of two wild edible plants (WEPs), Diplotaxis erucoides and Oxalis pes-caprae. Results showed interesting amounts of these bioactive compounds in the leaves, with intakes higher than the Recommended Daily Allowance (RDA) for vitamin A and vitamin E after consumption of 100 g. Diplotaxis erucoides and Oxalis pes-caprae leaves evidenced high amounts of carotenoids, such as lutein (about 8 mg/100 g and 5 mg, respectively) and β-carotene (about 8 mg/100 g and 4 mg/100 g, respectively). Even when not present at high amounts, the investigated plants can also contribute to the daily intake of thiamine and riboflavin. The rich profile and high contents of bioactive compounds in these WEPs clearly justify their potential use as food ingredients in a healthy and sustainable modern cuisine and in the development of new functional foods.

Notwithstanding the increased interest in wild edible plants, little is known on how some domestic thermal processes can affect their content. The aim of this study was to investigate the amounts of minerals, B1 and B2 vitamins, tocols, and carotenoids in raw, boiled, and steamed wild edible plants, namely, Sonchus asper (L.) Hill s.l., Sonchus oleraceus L., Cichorium intybus L., and Beta vulgaris L. var cicla. All vegetables were confirmed as high sources of lutein (from 6 to 9 mg/100 g) and β-carotene (from 2 to 5 mg/100 g). Quite high amounts of violaxanthin and neoxanthin were found. Alfa-tocopherol and γ-tocopherol were the main tocols, with same contents in raw and processed vegetables (about 2.5 mg/100 g). The most abundant macro element and trace element were, respectively, potassium and iron. B1 and B2 vitamins were found in low amounts in almost all plants, with the exception of thiamine in Beta vulgaris (about 1.6 mg/100 g). Boiling led to a significant loss of minerals (up to 60%) and B-group vitamins (up to 100%), while, among carotenoids, it only affected violaxanthin levels (up to 90%). Steamed vegetables showed only a slight reduction, about 20%, in β-carotene and lutein, with a marked decrease in violaxanthin and neoxanthin. One hundred grams of all fresh and cooked plants can be claimed as a source of vitamin A and E.

The presence of many different antioxidant molecules in foodstuffs and their by-products has attracted the scientific community's attention to their applications in finished foods due to their high bioactive power. Classes of molecules, such as phenolic compounds (phenolic acids, flavonoids, stilbenoids, and lignans), carotenoids, and tocols, provide numerous benefits to human health, reducing the incidence of cardiovascular disease, diabetes, and cancer. These molecules are innumerable and have similar but different molecular structures. Each can have a greater or lesser antioxidant power depending on its structure and its amounts in the finished food. Among these, tocols are a class of different homologues having antioxidant properties and are well represented in food. Request access from your librarian to read this chapter's full text.

In this study, a simple, fast, and effective methodology has been developed for the detection and quantification of seven phthalates potentially released in hot drinks from disposable containers used in vending machines. The authors determined the optimal conditions to be applied during the various steps of extraction of seven phthalates (DMP, DEP, DBP, DiBP, DEHP, DNOP, and DDP) from hot beverages using a model solution. The extraction and preconcentration technique used was ultrasound–vortex-assisted dispersive liquid–liquid microextraction (UVA-DLLME) followed by gas chromatographic analysis obtaining recoveries from 66.7% to 101.2% with precision and reproducibility −1 and 15.4 ng mL⁻¹ and limits of quantification (LOQs) from 1.6 ng mL⁻¹ to 35.8 ng mL⁻¹, both of them lower than those found by FID, were obtained.

This paper shows a characterization of the organic and inorganic fraction of river waters (Tiber and Marta) and ores/soil samples collected in the Northern Latium region of Italy for evaluating the anthropogenic/natural source contribution to the environmental pollution of this area. For organic compounds, organochloride volatile compounds in Tiber and Marta rivers were analyzed by two different clean-up methods (i.e., liquid–liquid extraction and static headspace) followed by gas chromatography–electron capture detector (GC-ECD) analysis. The results show very high concentrations of bromoform (up to 1.82 and 3.2 µg L⁻¹ in Tiber and Marta rivers, respectively), due to the presence of greenhouse crops, and of chloroform and tetrachloroethene, due to the presence of handicrafts installations. For the qualitative and quantitative assessment of the inorganic fraction, it is highlighted the use of a nuclear analytical method, instrumental neutron activation analysis, which allows having more information as possible from the sample without performing any chemical-physical pretreatment. The results have evidenced high levels of mercury (mean value 88.6 µg g⁻¹), antimony (77.7 µg g⁻¹), strontium (12,039 µg g⁻¹) and zinc (103 µg g⁻¹), whereas rare earth elements show levels similar to the literature data. Particular consideration is drawn for arsenic (414 µg g⁻¹): the levels found in this paper (ranging between 1 and 5100 µg g⁻¹) explain the high content of such element (as arsenates) in the aquifer, a big issue in this area.