Daniela Di Giacomo
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Daniela Di Giacomo has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Antonello Viola
Veterinary Practitioner, Centro Veterinario Nova Julia, Via Galileo Galilei 177, 64021 Giulianova, Italy
1 shared publication - Antonio Petrini
Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise “G. Caporale”, Campo Boario, 64100 Teramo, Italy
1 shared publication - Cesare Cammà
National Reference Center for Whole Genome Sequencing of Microbial Pathogens, Istituto Zooprofilattico Sperimentale Abruzzo-Molise “G. Caporale”, 64100 Teramo, Italy
1 shared publication - Daniela Malatesta
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Campo Boario, 64100 Teramo, Italy
1 shared publication - Giovanni Di Teodoro
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZSAM), Italy
1 shared publication - Marco Di Domenico
Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZSAM), 64100 Teramo, Italy
1 shared publication
Latest Publications
- Open Access
Validation of AmpliSeq NGS Panel for BRCA1 and BRCA2 Variant Detection in Canine Formalin-Fixed Paraffin-Embedded Mammary Tumors
Mammary carcinomas are the most common neoplasms observed in women and in female dogs. Canine mammary tumors show epidemiological, clinical, genetic, and prognostic characteristics comparable to human breast cancers. The recent introduction of next generation sequencing (NGS) technologies has greatly improved research and diagnostics for humans, while these new tools still need to be implemented in animal models. In this study we developed and validated an AmpliSeq Panel assay for the identification of BRCA variants in twenty-two different dogs. The amplicon mean coverage was 5499× and uniformity was higher than 98% in all samples. The results of germline single nucleotide variants (SNVs) and insertions/deletions (INDELs) were fully concordant regardless of the types of samples considered (blood, fresh and FFPE tissues). Moreover, despite the high DNA degradation observed in older FFPE blocks (>5 years), the assay allowed full coverage of all amplicons for downstream analyses. We consider the NGS panel developed in this study as a useful tool for expanding information on BRCA genes in the veterinary field and for human health from a comparative oncology perspective.
Open AccessValidation of AmpliSeq NGS Panel for BRCA1 and BRCA2 Variant Detection in Canine Formalin-Fixed Paraffin-Embedded Mammary Tumors
Mammary carcinomas are the most common neoplasms observed in women and in female dogs. Canine mammary tumors show epidemiological, clinical, genetic, and prognostic characteristics comparable to human breast cancers. The recent introduction of next generation sequencing (NGS) technologies has greatly improved research and diagnostics for humans, while these new tools still need to be implemented in animal models. In this study we developed and validated an AmpliSeq Panel assay for the identification of BRCA variants in twenty-two different dogs. The amplicon mean coverage was 5499× and uniformity was higher than 98% in all samples. The results of germline single nucleotide variants (SNVs) and insertions/deletions (INDELs) were fully concordant regardless of the types of samples considered (blood, fresh and FFPE tissues). Moreover, despite the high DNA degradation observed in older FFPE blocks (>5 years), the assay allowed full coverage of all amplicons for downstream analyses. We consider the NGS panel developed in this study as a useful tool for expanding information on BRCA genes in the veterinary field and for human health from a comparative oncology perspective.