Marcela Pacola
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Marcela Pacola has a total of 46 co-authors. Here are the top co-authors by number of shared publications:
- Carlos Alexandre Costa Crusciol
Department of Crop Science, College of Agricultural Sciences, São Paulo State University (UNESP), Botucatu 18610-034, SP, Brazil
7 shared publications - Lucas Moraes Jacomassi
Department of Crop Science College of Agricultural Sciences São Paulo State University (UNESP) Botucatu São Paulo Brazil
3 shared publications Gabriela Ferraz de Siqueira
Department of Crop Science, College of Agricultural Science São Paulo State University (UNESP), Botucatu, Brazil
2 shared publications- Gabriela Ferraz de Siqueira
Lageado Experimental Farm, Department of Crop Science, College of Agricultural Sciences, São Paulo State University (UNESP), P.O. Box 237, Botucatu 18610-307, SP, Brazil
2 shared publications - Josiane Viveiros
School of Agricultural Sciences (FCA), Sao Paulo State University (UNESP), Botucatu 18610-034, Brazil
2 shared publications - José Roberto Portugal
Department of Crop Science College of Agricultural Sciences São Paulo State University (UNESP) Botucatu São Paulo Brazil
2 shared publications
Latest Publications
- Open Access
- 16 September 2026
- Published by Elsevier in Rhizosphere
- Vol. 40
Multifunctional roles of Bacillus spp. and Paecilomyces lilacinum: Mechanisms of direct and indirect action in soybean and maize under tropical conditions
- 16 September 2026
- Published by Elsevier in Rhizosphere
- Vol. 40
Open AccessMultifunctional roles of Bacillus spp. and Paecilomyces lilacinum: Mechanisms of direct and indirect action in soybean and maize under tropical conditions
- 1 November 2025
- Published by Wiley in Agronomy Journal
- Vol. 117 (6)
Foliar nutrient application improves sugarcane bioenergy production by boosting photosynthetic enzyme activity and the antioxidant system
Sugarcane ( Saccharum spp. L.) is a globally important crop, and foliar fertilization can enhance plant growth, yield, and stress tolerance. However, the use of multi‐nutrient complexes remains underexplored. Here, we investigated the effect of two multi‐nutrient foliar fertilizer applications at vegetative stage (N, K, S, B, Cu, Mn, Mo and Zn) and at maturation stage (P, K, Mg, S and B) in 13 field experiments in the early, mid‐late, and late sugarcane harvest seasons. Four treatments were compared: (i) no application of multi‐nutrient foliar fertilizer (control), (ii) application at the vegetative stage of sugarcane (V), (iii) application at the maturation stage (M), and (iv) application at both the vegetative and maturation stages (VMs). Application resulted in significant gains in all biometric parameters, sugar yield, and theoretically recoverable sugars; the largest increases occurred in VM. Bagasse, straw, and energy production followed the pattern of biometric parameters and increased by averages of 7.9%, 9.7%, and 9.7% compared to the control. In early harvest sugarcane, phosphoenolpyruvate carboxylase, ribulose‐1,5‐bisphosphate carboxylase‐oxygenase, superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase activities and proline content were enhanced in M and VM, whereas hydrogen peroxide (H 2 O 2 ) and malondialdehyde contents decreased in these treatments. In summary, multi‐nutrient foliar fertilization at the VMs enhanced sugarcane growth, yield, energy production, and quality across all harvest seasons while improving photosynthetic and antioxidant enzyme activities. The results demonstrate that tailoring multi‐nutrient foliar fertilizer application to the specific physiological and nutritional demands of each phenological stage can promote sugarcane development by reducing the production of stress‐related molecules and enhancing carbon assimilation.
- 1 November 2025
- Published by Wiley in Agronomy Journal
- Vol. 117 (6)
Foliar nutrient application improves sugarcane bioenergy production by boosting photosynthetic enzyme activity and the antioxidant system
Sugarcane ( Saccharum spp. L.) is a globally important crop, and foliar fertilization can enhance plant growth, yield, and stress tolerance. However, the use of multi‐nutrient complexes remains underexplored. Here, we investigated the effect of two multi‐nutrient foliar fertilizer applications at vegetative stage (N, K, S, B, Cu, Mn, Mo and Zn) and at maturation stage (P, K, Mg, S and B) in 13 field experiments in the early, mid‐late, and late sugarcane harvest seasons. Four treatments were compared: (i) no application of multi‐nutrient foliar fertilizer (control), (ii) application at the vegetative stage of sugarcane (V), (iii) application at the maturation stage (M), and (iv) application at both the vegetative and maturation stages (VMs). Application resulted in significant gains in all biometric parameters, sugar yield, and theoretically recoverable sugars; the largest increases occurred in VM. Bagasse, straw, and energy production followed the pattern of biometric parameters and increased by averages of 7.9%, 9.7%, and 9.7% compared to the control. In early harvest sugarcane, phosphoenolpyruvate carboxylase, ribulose‐1,5‐bisphosphate carboxylase‐oxygenase, superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase activities and proline content were enhanced in M and VM, whereas hydrogen peroxide (H 2 O 2 ) and malondialdehyde contents decreased in these treatments. In summary, multi‐nutrient foliar fertilization at the VMs enhanced sugarcane growth, yield, energy production, and quality across all harvest seasons while improving photosynthetic and antioxidant enzyme activities. The results demonstrate that tailoring multi‐nutrient foliar fertilizer application to the specific physiological and nutritional demands of each phenological stage can promote sugarcane development by reducing the production of stress‐related molecules and enhancing carbon assimilation.
- Open Access
Enhancing Soybean Physiology and Productivity Through Foliar Application of Soluble Monoammonium Phosphate
Phosphorus (P) is essential for crop growth, but its complex behavior in tropical soils necessitates alternative management strategies, such as foliar supplementation. Foliar-applied nutrients act as biostimulants, enhancing stress tolerance and plant productivity. This study assessed the physiological responses of soybean to foliar application of soluble monoammonium phosphate (MAP; at a rate of 5 kg ha−1 each application) at different phenological stages (two during vegetative stages V4 and V6 and two during reproductive stages R1 and R3 or all four stages) across two growing seasons in tropical field conditions. Key parameters analyzed included leaf nutrient content, photosynthetic pigments, Rubisco activity, carbohydrate content, gas exchange (photosynthetic rate, stomatal conductance, transpiration, water use efficiency, and carboxylation efficiency), oxidative stress markers, and productivity indicators (100-grain weight and grain yield). MAP application improved all parameters, particularly at R1 and R3. Total chlorophyll increased by 29.2% at R1 and 30.0% when applied at all four stages, while the net photosynthetic rate rose by 15.8% and 18.4%, respectively. Water use efficiency improved by 20.0% at R1 and all four stages, while oxidative stress indicators, such as H2O2 levels, decreased. Rubisco activity increased most at R3 (46.0%) and all four stages (59.9%). Grain yield was highest with MAP spread at all four stages (12.3% increase), though a single application at R1 still boosted yield by 7.4%, compared to the control treatment.
Open AccessEnhancing Soybean Physiology and Productivity Through Foliar Application of Soluble Monoammonium Phosphate
Phosphorus (P) is essential for crop growth, but its complex behavior in tropical soils necessitates alternative management strategies, such as foliar supplementation. Foliar-applied nutrients act as biostimulants, enhancing stress tolerance and plant productivity. This study assessed the physiological responses of soybean to foliar application of soluble monoammonium phosphate (MAP; at a rate of 5 kg ha−1 each application) at different phenological stages (two during vegetative stages V4 and V6 and two during reproductive stages R1 and R3 or all four stages) across two growing seasons in tropical field conditions. Key parameters analyzed included leaf nutrient content, photosynthetic pigments, Rubisco activity, carbohydrate content, gas exchange (photosynthetic rate, stomatal conductance, transpiration, water use efficiency, and carboxylation efficiency), oxidative stress markers, and productivity indicators (100-grain weight and grain yield). MAP application improved all parameters, particularly at R1 and R3. Total chlorophyll increased by 29.2% at R1 and 30.0% when applied at all four stages, while the net photosynthetic rate rose by 15.8% and 18.4%, respectively. Water use efficiency improved by 20.0% at R1 and all four stages, while oxidative stress indicators, such as H2O2 levels, decreased. Rubisco activity increased most at R3 (46.0%) and all four stages (59.9%). Grain yield was highest with MAP spread at all four stages (12.3% increase), though a single application at R1 still boosted yield by 7.4%, compared to the control treatment.
- Open Access
Foliar Application of Amino Acids and Nutrients as a Tool to Mitigate Water Stress and Stabilize Sugarcane Yield and Bioenergy Generation
Extended periods of water stress negatively affect sugarcane crop production. The foliar application of supplements containing specific nutrients and/or organic molecules such as amino acids can improve sugarcane metabolism, stalk and sugar yields, and the quality of the extracted juice. The present study assessed the effectiveness of the foliar application of an abiotic stress protection complement (ASPC) composed of 18 amino acids and 5 macronutrients. The experiments were carried out in the field with two treatments and twelve replicates. The two treatments were no application of ASPC (control) and foliar application of ASPC. The foliar application of ASPC increased the activity of antioxidant enzymes. The Trolox-equivalent antioxidant capacity (DPPH) was higher in ASPC-treated plants than in control plants, reflecting higher antioxidant enzyme activity and lower malondialdehyde (MDA) levels. The level of H2O2 was 11.27 nM g−1 protein in plants treated with ASPC but 23.71 nM g−1 protein in control plants. Moreover, the application of ASPC increased stalk yield and sucrose accumulation, thus increasing the quality of the raw material. By positively stabilizing the cellular redox balance in sugarcane plants, ASPC application also increased energy generation. Therefore, applying ASPC is an effective strategy for relieving water stress while improving crop productivity.
Open AccessFoliar Application of Amino Acids and Nutrients as a Tool to Mitigate Water Stress and Stabilize Sugarcane Yield and Bioenergy Generation
Extended periods of water stress negatively affect sugarcane crop production. The foliar application of supplements containing specific nutrients and/or organic molecules such as amino acids can improve sugarcane metabolism, stalk and sugar yields, and the quality of the extracted juice. The present study assessed the effectiveness of the foliar application of an abiotic stress protection complement (ASPC) composed of 18 amino acids and 5 macronutrients. The experiments were carried out in the field with two treatments and twelve replicates. The two treatments were no application of ASPC (control) and foliar application of ASPC. The foliar application of ASPC increased the activity of antioxidant enzymes. The Trolox-equivalent antioxidant capacity (DPPH) was higher in ASPC-treated plants than in control plants, reflecting higher antioxidant enzyme activity and lower malondialdehyde (MDA) levels. The level of H2O2 was 11.27 nM g−1 protein in plants treated with ASPC but 23.71 nM g−1 protein in control plants. Moreover, the application of ASPC increased stalk yield and sucrose accumulation, thus increasing the quality of the raw material. By positively stabilizing the cellular redox balance in sugarcane plants, ASPC application also increased energy generation. Therefore, applying ASPC is an effective strategy for relieving water stress while improving crop productivity.
- 13 June 2023
- Published by Wiley in Soil Use and Management
- Vol. 39 (4) , 1388-1402
Soil tillage systems and forms of lime application on soil attribute enhancements in ratoon sugarcane
Combining deep tillage (DT) with lime application at greater depths may improve sugarcane yield. The objective of this study was to evaluate the impact of conventional tillage (CT) and DT systems and liming on sugarcane productivity and soil physical attributes. The experiment was conducted in a clayey‐textured Rhodic Hapludox soil cultivated with sugarcane for two growing seasons (first and third ratoons) using a randomized block design with four treatments and four repetitions. The treatments consisted of DT without liming (DT0), DT with liming (DT2), CT without liming (CT0) and CT with liming (CT2). In addition to sugarcane stalk and sugar yields, macroporosity (MA), microporosity (MI), total soil porosity (TP), weighted average diameter (WAD), aggregate stability index (ASI), soil penetration resistance (SPR), pH, potential acidity (H + Al), total soil organic carbon (SOC), particulate organic carbon (POC), mineral‐associated organic carbon (MOC), calcium (Ca) and magnesium (Mg) were analysed. DT2 promoted the best soil conservation effect on sugarcane. In general, DT, regardless of lime application, reduced SOC and POC in soil surface layer. Nevertheless, in the long term, localized lime addition resulted in significant reductions in compaction, reaching values below 2 MPa. The soil fertility improvement provided by DT2 promoted increased sucrose concentrations and stalks yield. Considering that there was an improvement in the physical quality and fertility of soil for better plant development, the deep tillage with localized lime addition can be considered an effective alternative for sugarcane cultivation.
- 13 June 2023
- Published by Wiley in Soil Use and Management
- Vol. 39 (4) , 1388-1402
Soil tillage systems and forms of lime application on soil attribute enhancements in ratoon sugarcane
Combining deep tillage (DT) with lime application at greater depths may improve sugarcane yield. The objective of this study was to evaluate the impact of conventional tillage (CT) and DT systems and liming on sugarcane productivity and soil physical attributes. The experiment was conducted in a clayey‐textured Rhodic Hapludox soil cultivated with sugarcane for two growing seasons (first and third ratoons) using a randomized block design with four treatments and four repetitions. The treatments consisted of DT without liming (DT0), DT with liming (DT2), CT without liming (CT0) and CT with liming (CT2). In addition to sugarcane stalk and sugar yields, macroporosity (MA), microporosity (MI), total soil porosity (TP), weighted average diameter (WAD), aggregate stability index (ASI), soil penetration resistance (SPR), pH, potential acidity (H + Al), total soil organic carbon (SOC), particulate organic carbon (POC), mineral‐associated organic carbon (MOC), calcium (Ca) and magnesium (Mg) were analysed. DT2 promoted the best soil conservation effect on sugarcane. In general, DT, regardless of lime application, reduced SOC and POC in soil surface layer. Nevertheless, in the long term, localized lime addition resulted in significant reductions in compaction, reaching values below 2 MPa. The soil fertility improvement provided by DT2 promoted increased sucrose concentrations and stalks yield. Considering that there was an improvement in the physical quality and fertility of soil for better plant development, the deep tillage with localized lime addition can be considered an effective alternative for sugarcane cultivation.
- 14 June 2022
- Published by Wiley in Pest Management Science
- Vol. 78 (10) , 4006-4017
Phytotonic effects of thiamethoxam on sugarcane managed with glyphosate as a ripener
BACKGROUND: Thiamethoxam and glyphosate are widely used in sugarcane production as an insecticide and ripener, respectively. In this study, the potential of these chemical products to also elicit phytotonic effects and enhance the physiological development and yield of sugarcane was evaluated. In field experiments, thiamethoxam and glyphosate were applied to sugarcane individually or in combination, and the effects of these chemical management strategies on sugarcane biometric and technological parameters were assessed.RESULTS: Thiamethoxam application improved biometric parameters, especially stalk yield. Glyphosate application increased sugar yield, despite reducing the number of stalks and consequently the stalk yield.CONCLUSIONS: Application of the insecticide thiamethoxam to sugarcane attenuates the depreciative effect of ripener (glyphosate) and has a potential phytotonic effect by increasing sugar yields in the early and late seasons. © 2022 Society of Chemical Industry.
- 14 June 2022
- Published by Wiley in Pest Management Science
- Vol. 78 (10) , 4006-4017
Phytotonic effects of thiamethoxam on sugarcane managed with glyphosate as a ripener
BACKGROUND: Thiamethoxam and glyphosate are widely used in sugarcane production as an insecticide and ripener, respectively. In this study, the potential of these chemical products to also elicit phytotonic effects and enhance the physiological development and yield of sugarcane was evaluated. In field experiments, thiamethoxam and glyphosate were applied to sugarcane individually or in combination, and the effects of these chemical management strategies on sugarcane biometric and technological parameters were assessed.RESULTS: Thiamethoxam application improved biometric parameters, especially stalk yield. Glyphosate application increased sugar yield, despite reducing the number of stalks and consequently the stalk yield.CONCLUSIONS: Application of the insecticide thiamethoxam to sugarcane attenuates the depreciative effect of ripener (glyphosate) and has a potential phytotonic effect by increasing sugar yields in the early and late seasons. © 2022 Society of Chemical Industry.
- Open Access
- 19 January 2021
- Published by Portal de Periódicos UFU - Universidade Federal de Uberlandia in Bioscience Journal
- Vol. 37, e37010
Cover crops, lime and gypsum influence on soil physical attributes
Cover crops promote nutrient cycling, and lime and gypsum can alter the soil physical attributes. This study aimed to evaluate the effect of lime and gypsum rates applied to a no-tillage system with addition of residues of three cover crops on the soil physical attributes. This experiment was carried out in chapadão do sul-ms. The treatments were comprised of three cover crops (Urochloa ruziziensis, fallow, and Pennisetum glaucum), with gypsum (0, 2.3 and 4.6 Mg ha-1) and lime applied at a dose of 0, 2, 4, 6 Mg ha-1).The attributes evaluated were: soil density, macroporosity, microporosity, total porosity and penetration resistance. The soil of the experiment was classified an Oxisol. Cover crops and lime and gypsum improved macroporosity, microporosity and total porosity at all depths, 0-0.2 m. Millet presented lower values for penetration resistance with the lime application and without gypsum application. No residual effect on soil density was detected for lime and gypsum application or cover crops in the 0.1-0.2 m layer. Brazilian Cerrado producers will have a well-defined management system to follow aiming at improving the soil physical attributes.
- 19 January 2021
- Published by Portal de Periódicos UFU - Universidade Federal de Uberlandia in Bioscience Journal
- Vol. 37, e37010
Open AccessCover crops, lime and gypsum influence on soil physical attributes
Cover crops promote nutrient cycling, and lime and gypsum can alter the soil physical attributes. This study aimed to evaluate the effect of lime and gypsum rates applied to a no-tillage system with addition of residues of three cover crops on the soil physical attributes. This experiment was carried out in chapadão do sul-ms. The treatments were comprised of three cover crops (Urochloa ruziziensis, fallow, and Pennisetum glaucum), with gypsum (0, 2.3 and 4.6 Mg ha-1) and lime applied at a dose of 0, 2, 4, 6 Mg ha-1).The attributes evaluated were: soil density, macroporosity, microporosity, total porosity and penetration resistance. The soil of the experiment was classified an Oxisol. Cover crops and lime and gypsum improved macroporosity, microporosity and total porosity at all depths, 0-0.2 m. Millet presented lower values for penetration resistance with the lime application and without gypsum application. No residual effect on soil density was detected for lime and gypsum application or cover crops in the 0.1-0.2 m layer. Brazilian Cerrado producers will have a well-defined management system to follow aiming at improving the soil physical attributes.