Daniela Gonzalez
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Daniela Gonzalez has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
- Cameron Klein
University of Nebraska-Lincoln
1 shared publication - Charles Wood
Department of Interdisciplinary Oncology, Louisiana State University Health Sciences Center
1 shared publication - Crispin Kahesa
Ocean Road Cancer Institute, Dar es Salaam, Tanzania
1 shared publication - John T. West
Nebraska Center for Virology, Lincoln, NE, USA; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA
1 shared publication - Julius Mwaiselage
Ocean Road Cancer Institute, Dar es Salaam P.O. Box 3592, Tanzania
1 shared publication - Nirosh Aluthge
University of Nebraska-Lincoln
1 shared publication
Latest Publications
- Open Access
The Relationship Between the Cervical Microbiome, HIV Status, and Pre-Cancerous Lesions
Nearly all cervical cancers are causally associated with Human Papillomavirus (HPV). The burden of HPV-associated dysplasias in Sub-Saharan Africa is influenced by HIV. To investigate the role of the bacterial microbiome in cervical dysplasia, cytobrush samples were collected directly from cervical lesions of 144 Tanzanian women. The V4 hypervariable region of the 16S rRNA gene was amplified and deep-sequenced. Alpha diversity metrics; Chao1, PD whole tree, and operational taxonomic Unit (OTU) estimates, displayed significantly higher bacterial richness in HIV positive patients (P = 0.01) than in HIV negative patients. Within HIV positive patients, there was higher bacterial richness in patients with high grade squamous intraepithelial lesions (HSIL; P = 0.13) than those without lesions. The most abundant OTUs associated with high-grade squamous intraepitheilal lesions (HSIL) were Mycoplasmatales, Pseudomonadales , and Staphylococcus . We suggest that a chronic mycoplasma infection of the cervix can contribute to HPV-dependent dysplasia by sustained inflammatory signals.
Open AccessThe Relationship Between the Cervical Microbiome, HIV Status, and Pre-Cancerous Lesions
Nearly all cervical cancers are causally associated with Human Papillomavirus (HPV). The burden of HPV-associated dysplasias in Sub-Saharan Africa is influenced by HIV. To investigate the role of the bacterial microbiome in cervical dysplasia, cytobrush samples were collected directly from cervical lesions of 144 Tanzanian women. The V4 hypervariable region of the 16S rRNA gene was amplified and deep-sequenced. Alpha diversity metrics; Chao1, PD whole tree, and operational taxonomic Unit (OTU) estimates, displayed significantly higher bacterial richness in HIV positive patients (P = 0.01) than in HIV negative patients. Within HIV positive patients, there was higher bacterial richness in patients with high grade squamous intraepithelial lesions (HSIL; P = 0.13) than those without lesions. The most abundant OTUs associated with high-grade squamous intraepitheilal lesions (HSIL) were Mycoplasmatales, Pseudomonadales , and Staphylococcus . We suggest that a chronic mycoplasma infection of the cervix can contribute to HPV-dependent dysplasia by sustained inflammatory signals.