Yanting Li

Glucose and its derivatives serve as key carbon sources and signaling molecules in plants, yet how structurally distinct carbohydrates differentially influence crop growth remains unclear. Our study employed glucose (Glc, -CHO), sorbitol (Sbt, -OH), gluconic acid (GlcA, -COOH), and glucuronic acid (GroA, -COOH; -CHO) as treatment factors, thereby maximizing variation in oxygen-containing functional groups while keeping the C₆ carbon backbone constant. A completely randomized hydroponic experiment was established with four concentration levels (0, 10, 25, and 50 mg L⁻¹) to systematically elucidate how glucose and its derivatives influence wheat growth and nutrient uptake, and to clarify the role of functional group chemistry in the performance of carbon-based bio-stimulants. All carbohydrate treatments significantly improved wheat dry matter (17.50–35.00%) and nutrient uptake (N: 17.12–32.18%, P: 21.73–36.97%, K: 12.28–30.51%) compared with the control, primarily by promoting root vigor and nutrient absorption. Among the four carbohydrates, Glc showed the greatest stimulatory effect, significantly enhancing root vigor. Sbt primarily facilitated root elongation and surface area, whereas GlcA and GroA were slightly less effective but still promoted nutrient uptake. The optimal concentrations were 10 mg L⁻¹ for Glc and 25 mg L⁻¹ for its derivatives. These findings provide practical guidance for enhancing nutrient use efficiency and crop productivity using structurally targeted carbohydrates for sustainable agriculture.

Due to its broad yield-increasing effect and low cost, humic acid urea (HAU) has become one of the leading modified fertilizers worldwide. The fertisphere is the primary space where urea (U) granules participate in the soil nitrogen cycle, forming a nutrient concentration gradient centered on the point of fertilization. The closer the circle layers to the urea granule in the fertisphere, the higher the nitrogen concentration. However, HAU in this microregion remains poorly understood. The differences in the transformation process from the inside to outside circle layers of the U and HAU fertispheres were simulated and studied using soil incubation experiments under 20, 10, 2, 1, and 0.2 g kg⁻¹ nitrogen inputs. The 20 and 10 g kg⁻¹ inputs represent the layers closest to the urea granule. Within the first seven days, HAU treatment showed higher concentrations of soil ammonia-N content than U treatment within the two layers closest to the fertilizer core, while exhibiting lower concentrations under the farthest two layers. Under 2 g kg⁻¹ nitrogen input, the nitrate nitrogen under the HAU treatment was significantly higher than that in the U treatment, indicating a higher nitrification rate. During the 42-day incubation period, soil mineral nitrogen content under the HAU treatment was higher than that for the U treatment in the two closest circles. On the 42nd day, the residual urea-N under the HAU treatment was significantly higher than that for the U treatment when the nitrogen input was higher than 1 g kg⁻¹. The effect of higher fertilizer preservation and supply capacity of HAU in Fluvo-aquic soil was achieved by changing the urease activity and nitrification rate in fertisphere ranges closer to the fertilizer core. An improved understanding of the high-efficiency mechanism of HAU in the fertisphere process will contribute to the development of new-generation high-efficiency urea products.

Polyaspartic acid (PASP) is widely used in agriculture owing to its role in promoting crop growth and nutrient absorption, and its effects are correlated with its molecular weight. In this study, a field soil column experiment using a ¹⁵N tracer was conducted to compare the effects of urea enhanced by PASPs with different molecular weights on wheat yield, nitrogen uptake by wheat, and nitrogen residue in the soil. The results showed that urea enhanced with PASPs (PASPUs) increased the wheat yield by 2.02–9.77% than conventional urea (CU) through enhancing the wheat spike number. PASPUs promoted wheat nitrogen uptake, and urea enhanced with PASPs of high molecular weight (>10 kDa) significantly promoted total nitrogen uptake, fertilizer–N uptake, and soil–N uptake by 9.92%, 4.65%, and 19.62%, respectively, compared with CU. PASPUs reduced fertilizer–N leaching by increasing its residue in the 30–60 cm soil layer and decreasing its amount in the 60–90 cm soil layer. A comprehensive evaluation based on radar charts showed that PASPs of high molecular weight showed a significant improvement in wheat yield and nitrogen–use efficiency, compared with CU and urea enhanced with other molecular weights, and is the most recommended PASP fraction for enhancing nitrogen utilization.

Incorporating humic acid (HA) into phosphate fertilizers to produce HA-enhanced phosphate fertilizers (HAPs) can improve the migration and availability of fertilizer-derived phosphorus (P) in soil. However, the optimal proportion of HA remains inconsistent. Here, we investigated the effects of HAPs with different HA proportions (0.1–10% w/w) on water-soluble P fixation rate, P migration, P transformation, and soil microorganisms, and analyzed the main P forms in HAP using Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The results showed that incorporating 0.1% HA had no impact on P migration and transformation, whereas incorporating 0.5–10% HA increased the migration distance and cumulative migration of fertilizer-derived P by 0–5 mm and 17.1–30.3%, respectively, compared with conventional phosphate fertilizer (CP). Meanwhile, HAPs with 0.5–10% HA significantly reduced the water-soluble P fixation rate by 18.3–25.6%, and significantly increased the soil average available P (AP) content in 0–40 mm soil layer around the P application site by 6.2–12.9% relative to CP, partly due to the phosphate monoesters in HAPs. Clustering analysis revealed that 0.5% HA had similar effects relative to higher HA proportions (1% and 5%), and the inhibition of HAP with 0.5% HA on bacteria and fungi was also greater than that of CP due to the high concentration of soil P. Overall, 0.5% was determined to be the optimal amount of HA for HAP production, which provided a theoretical basis for the development of high-efficiency phosphate fertilizer.

Saline-alkali stress adversely affects crop growth and nutrient absorption, thereby reducing crop yield and quality. Exogenous amino acids have multiple synergistic effects; however, whether a preparation containing amino acids increases the resistance of pakchoi to saline-alkali soil remains unclear. This study investigated the effects of a patented “preparation” containing amino acids on crop growth, nutrient absorption, and tolerance to saline-alkali stress in pakchoi (Brassica chinensis L.), using a pot culture experiment with three successive plantings. The preparation was made using tail liquor generated during the production of monosodium glutamate. Topdressing with the preparation had little effect on pakchoi growth, yield, quality, or nutrient accumulation, compared with no topdressing treatment. However, the addition of the preparation to water-soluble fertiliser increased crop yield by 11.9–17.6%, with a 15.4% cumulative increase over the three crops. The concentrations of vitamin C, soluble sugar, and soluble protein increased by 10.2–12.4%, 11.9–22.3%, and 3.0–14.3%, respectively. The fertiliser utilisation ratio for the three crops increased by 30.5%, while the nitrate content decreased by 8.5–20.4%. The fresh weight significantly decreased when the dosage of water-soluble fertiliser was reduced to 20%. However, the addition of the preparation to the reduced-dosage water-soluble fertiliser compensated for this reduction, especially in the third crop. Our results show that the preparation improves pakchoi resistance to saline-alkali stress and restores agricultural productivity.

Land application of livestock manure may reduce the use of mineral fertilizers and alleviate the environmental degradation associated with mineral fertilizers application. However, how to optimize utilization of livestock manure value is not well understood and documentation regarding the nitrogen (N) and phosphorus (P) fertilizer replacement values (NFRV and PFRV, respectively) needs further scrutiny. Therefore, three representative livestock manures, i.e., pig, chicken, and cattle manure, were applied at different usages to assess their N and P availability in comparison to reference mineral fertilizers over summer maize growing seasons. The results show that the average NFRVs of pig, chicken, and cattle manures were 41.7–58.4%, 27.5–44.4%, and −3.6–36.1%, respectively, when based on different references (grain yield, total dry matter yield, grain N uptake, total N uptake), at different N application levels. The NFRV increased with the elevated N application rate for cattle manure treatment. In the P trials, livestock manure had a higher PFRV at a low P application level, and the average PFRVs of pig, chicken, and cattle manures were 80.3–164.8%, 77.9–143.7%, and 94.1–168.0%, respectively, at different P application levels. We conclude that livestock manure produced the lowest NFRV and highest PFRV at a low fertilizer application rate; pig manure had the highest N availability; and cattle manure had the highest P availability.

Weathered coal is known to have potential inhibitory effects on urease activity, thus reducing the loss of nitrogen from fertilizer such as ammonia. This means that it can be used as a urea enhancer to promote urea utilization efficiency. However, the variation in its composition and structure has impeded the optimal utilization of this resource. In this study, we collected Chinese weathered coal from six representative geographical locations and analyzed its elemental and substance composition, as well as determined its chemical structure via Fourier transform infrared spectroscopy and investigated its effects on urease (soybean meal) activity. The results showed evident variation in the composition and structure among the different weathered coal samples, especially in the pH values, humic acid and ash content, and aromaticity. All six weathered coal samples significantly inhibited urease activity, and the inhibitory effect was enhanced with the elevated proportion of weathered coal introduced to urea. When the additive proportion of weathered coal increased, the weathered coal, characterized as having a higher humic acid content and a more aliphatic structure, showed a more rapid increase in the urease activity inhibition rate, while there was only a slight effect when the weathered coal had a low humic acid content and high atomicity. Therefore, the former type of weathered coal was more sensitive to the additive proportion. Furthermore, there was no consistent rule when the same proportion of weathered coal from different geographic locations was blended into urea, which might be attributable to other unexplored factors.

Humic acids (HAs) incorporated into urea fertilizers are highly effective at increasing yield and decreasing fertilizer-derived nitrogen (N) loss from soil, but reports of the optimal proportion in fertilizers remain widely inconsistent. In this study, we examined the effects of urea enhanced with 0.2–5.0% HAs (UHAs) on the yield, biomass production, N uptake, and N residue in fluvo-aquic soil in winter wheat cultivated over two growing seasons from 2018 to 2020 in the North China Plain. UHAs application significantly enhanced wheat grain yield, aboveground dry biomass, total and fertilizer-derived N uptake by wheat, and residue in soil, while reducing the loss of fertilizer-derived N. Additionally, UHAs treatments increased fertilizer-N residues in soil, especially in the top 30 cm soil layer, which increased with the proportion of added HAs. These positive effects were attributed to a higher spike number under UHAs treatments compared to conventional urea. Clustering analysis of the different treatments showed that 0.2% HAs were more similar to conventional urea, while 0.5% had similar effects to HAs at higher proportions. UHAs application significantly enhanced wheat grain yield, mainly via increasing spike number, and optimized the fertilizer-N fate. Among UHAs treatments, 0.5% HAs showed the highest increase in economic benefit.