Jun Liu

Cadmium is a common reproductive toxin in aquatic systems. Cd exposure of fish species at high concentrations can severely affect the reproductive function of fish. However, the underlying toxicity of cadmium exposure at low concentrations on the reproductive function in parental fish remains unclear. To investigate the impacts of cadmium exposure on reproductive capability, eighty-one male and eighty-one female rare minnows (Gobiocypris rarus) were exposed to cadmium at 0 (control group), 5 and 10 μg/L for 28 days, and then transferred into clean water to pair spawn. The results showed that cadmium exposure at 5 or 10 μg/L for 28 days in rare minnows could reduce the success rates of pair spawning in parent rare minnows, lessen no-spawning activities, and prolong the time for first spawning. Furthermore, the mean egg production of the cadmium exposure group increased. The fertility rate of the control group was significantly higher than that of the 5 μg/L cadmium exposure group. Anatomical and histological data further revealed that the intensity of atretic vitellogenic follicles significantly increased and spermatozoa vacuolated after cadmium exposure (p< 0.05), but slightly increased the condition factor (CF), and relatively stable gonadosomatic index (GSI) values were also observed in the cadmium exposure groups. These observed results indicated that cadmium exposure at 5 or 10 μg/L affected the reproductive activity of paired rare minnow by accumulating Cd in the gonads, and the effect diminished over time. The reproductive risk of low-dose cadmium exposure to fish species remains a cause for concern.

The wide use of rare-earth elements in China for aquacultural purposes and many other applications has resulted in their accumulation in the aquatic environment and has caused concern about their safety. In this study, we tested the toxicity of lanthanum (La (III)) to the early life stages (embryonic and sac-fry stages) of the rare minnow Gobiocypris rarus Ye & Fu, 1983. We exposed fertilized eggs to 0, 0.06, 0.13, 0.25, 0.50, 1.00, and 1.92 mg/L of La (III) until the yolk sac was exhausted in any group (at about 168 h of exposure). Exposure to 1.00 and 1.92 mg/L La (III) had obvious lethal effects on embryos, La (III) exposure also accelerated the development of embryos and had a significant inhibitory effect on the hatching rate after 96 h. As the exposure time increased, the larvae exhibited obvious yolk-sac edema, pericardium edema, spinal curvature, tail bending, and other symptoms of poisoning, including deflated swim-bladder. In general, these results clearly indicate that La pollutants hinder the development of rare minnow embryos and are acutely toxic to rare minnow larvae. Our finding would provide a theoretical basis for further research of relevant feed additive criteria for this fish.