Mickaël Bejean

Reintroduction programmes are increasingly used to restore threatened freshwater fishes, yet evaluating their success remains challenging during the early stages of population establishment. Genetic monitoring is commonly used to assess diversity but rarely provides direct evidence of natural reproduction. Here, we investigated natural reproduction following reintroduction in the endangered Zingel asper, a benthic fish endemic to the Rhône River basin. Between 2006 and 2015, more than 25,000 captive-bred individuals from two source populations were released into the Drôme River (France). Using 54 microsatellite loci, we assessed genetic diversity, effective population size, relatedness and inbreeding, reconstructed parentage relationships, and evaluated admixture among source lineages through hybrid assignment analyses. Wild source populations differed markedly in genetic diversity and effective population size, whereas captive propagation reduced genetic diversity and increased relatedness and inbreeding. Despite these effects, the reintroduced population exhibited higher allelic richness than any captive cohort. Parentage analyses provided direct genetic evidence of natural reproduction by identifying individuals whose genetic origin could not be explained by the captive breeding pedigree. Hybrid assignment analyses independently confirmed admixture between source lineages. These results show how complementary genetic approaches can provide early evidence of reintroduction success and support adaptive management of threatened fish populations.

The freshwater jellyfish Craspedacusta sowerbii is among the most widespread invasive species, observed across a wide temperature range. The aim of this study is to analyze the polyp and medusa stages response to different temperatures by using (i) an experimental study on the polyp colony growth at 19 and 29 °C, and (ii) prediction of the Thermal Habitat Suitability (THS) based on the thermal tolerance of the medusa stage. The total number of polyps and colonies was greater at high temperature. At 19 °C, colonies with 1 to 5 polyps were present, with colonies of 1 to 3 polyps numerically dominating. At 29 °C, colonies were 80% composed of 1- to 2-polyps. Based on the published medusa pulsation rhythm data, a Thermal Performance Curve (TPC) regression was performed and used to monthly predict the THS for current and future (2050 and 2100) scenarios. The southern hemisphere offered optimal conditions (THS > 0.6) year-round. In the northern hemisphere, the optimum period was predicted to be between June and September. The future THS were considerably larger than at present with an increase in optimal THS at higher latitudes (up to 60° N). The combination of experimental and modeling approaches allows to identify the optimal thermal conditions of the polyp and medusa stages and to predict their invasive capacities.