Alfredo Márquez-Lucero

Herein, a methodology is employed based on the Flory–Rehner equation for estimating the Flory–Huggins interaction parameter (χ₁₂*) of crosslinked elastomer blends. For this purpose, binary elastomer blends containing polybutadiene rubber (BR), styrene–butadiene rubber (SBR) and nitrile–butadiene rubber (NBR), were prepared in a mixing chamber at a temperature below the activation of the crosslinking agent. Swelling tests with benzene were employed to determine the crosslinked fraction, finding that after 20 min of thermal annealing, the BR and NBR were almost completely crosslinked, while the SBR only reached 60%. Additionally, the BR-SBR blend increased by 2–3 times its volume than its pure components; this could be explained based on the crosslink density. From the mechanical tests, a negative deviation from the rule of mixtures was observed, which suggested that the crosslinking was preferably carried out in the phases and not at the interface. Furthermore, tensile tests and swelling fraction (ϕ_sw) results were employed to determine the average molecular weight between two crosslinking points (M_c), and subsequently χ₁₂*. Calculated χ₁₂* values were slightly higher than those reported in the literature. The calculated thermodynamic parameters for the blends showed positive ΔG_mix values and endothermic behavior, suggesting their immiscible nature.

Nafion is a sulfonated tetrafluoroethylene-based fluoropolymer-copolymer, which is an excellent material for fuel cell membranes. This widely used copolymer has been investigated for decades, but it has been hardly considered as a sensitive layer to organic vapours, but only as a conductor of electricity. However, its sensitive properties make it an interesting material for sensor applications. Indeed, the mass production of this material open new developments to design reliable and cheap sensors, useful for military, industrial and medical applications. In this work, we study Nafion (and eventually other ionomers) physical and transport response when it enters in contact with ketone vapours, on the perspective to design new chemical sensors, more compact and cheaper. This work shows the possibility to employ sulfonated tetrafluoroethylene-based fluoropolymer-copolymer, commercially known as Nafion, as a sensible layer on sensors to detect organic solvents such as ketones. The detection and evaluation of ketone corpuses is very important for multiple applications on medicine, specially to detect and evaluate Diabetes Mellitus from the breath of patients. Nafion is a very stable copolymer, easily available and relatively inexpensive. This allows us to envision the possibility of having cheap and reliable sensors to detect vapours of these substances based on this copolymer. The main result of the present work is that; Nafion can protonate gaseous ions from organic solvents, such as acetone and similar substances, which modify its electrical properties, presenting a differentiated behavior according to the chemical nature of these substances, which could lead to their identification, designing an electrical nose, because each behaviour is a fingerprint of the substance to detect. Then this material can be used in the design of electrical sensors, which can be low-cost, reliable, and chemically stable, representing an excellent alternative to ceramic sensors.

This work shows the possibility to employ sulfonated tetrafluoroethylene-based fluoropolymer-copolymer, commercially known as Nafion, as a sensible layer on sensors to detect organic solvents such as ketones. The detection and evaluation of ketone corpuses is very important for multiple applications on medicine, specially to detect and evaluate diabetes mellitus from the breath of patients. Nafion is a very stable copolymer, easily available and relatively inexpensive. This allows us to envision the possibility of having cheap and reliable sensors to detect vapors of these substances based on this copolymer. The main result of the present work is that Nafion can protonate gaseous ions from organic solvents, such as acetone and similar substances, which modify its electrical properties, presenting a differentiated behavior according to the chemical nature of these substances, which could lead to their identification, designing an electrical nose, because each behavior is a fingerprint of the substance to detect. Then, this material can be used in the design of electrical sensors, which can be inexpensive, reliable, and chemically stable, representing an excellent alternative to ceramic sensors.

The high incidence of Diabetes Mellitus in low-income regions has promoted the development of low-cost alternatives to replace blood-based procedures. In this work, we present a bienzymatic paper-based sensor suitable for the naked-eye detection of glucose in saliva samples. The sensor was obtained by a stamping procedure and modified with chitosan to improve the colorimetric readout. The bienzymatic reaction of GOx-HRP coupled with 2,4,6-tribromo-3-hydroxy benzoic acid was applied for the detection of glucose within a range from 0 to 180 mgdL⁻¹ in buffer and artificial saliva solutions. The visual readout was perceived by the naked eye and registered with an office scanner to evaluate the analytical performance. The results showed a limit of detection of 0.37 mgdL⁻¹ (S/N = 3) with an R.S.D. of 1.69% and a linear range from 1 to 22.5 mgdL⁻¹ with an R² of 0.99235. The analysis of human saliva samples was performed without pre-processing, achieving recoveries from 92 to 114%. The naked-eye detection was evaluated under two different light settings, showing average recoveries of 108.58 and 90.65% for standard and low illumination. The proposed device showed potential for easy-to-use, sensitive, low-cost, fast, and device-free detection of salivary glucose suitable for untrained personnel operation and limited facilities.

Given the limited access to healthcare resources, low-income settings require the development of affordable technology. Here we present the design and evaluation of a low-cost colorimeter applied to the non-invasive monitoring of Diabetes Mellitus through the detection of glucose in salival fluid. Samples were processed by the glucose oxidase-peroxidase enzymatic system and analyzed with the development equipment. A light emission diode of 532.5 nm was used as an excitation source and a RGB module was used as a receptor. A calibration curve to quantify the concentration of salivary glucose (0 to 18 mg/dL) was carried out by relating the RGB components registered with glucose concentrations, achieving a limit of detection of 0.17 mg/dL with a CV of 5% (n = 3). Salivary samples of diabetic and healthy volunteers were processed with the equipment showing an average concentration of 1.5519 ± 0.4511 mg/dL for the first and 4.0479 ± 1.6103 mg/dL for the last, allowing a discrimination between both groups. Results were validated against a UV-Vis-NIR spectrophotometer with a correspondence of R² of 0.98194 between both instruments. Results suggest the potential application of the developed device to the sensitive detection of relevant analytes with a low-cost, user-friendly, low-power and portable instrumentation.