Xiqiang Liu
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Xiqiang Liu has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Guiqin Zhao
Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China
1 shared publication - Lin Ma
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
1 shared publication - Pan Gong
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
1 shared publication - Wenlong Gong
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China
1 shared publication - Zan Wang
College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China
1 shared publication
Latest Publications
- 15 November 2018
- Published by Springer Nature in Molecular Biology Reports
- Vol. 46 (1) , 1323-1326
Development and application of EST–SSRs markers for analysis of genetic diversity in erect milkvetch (Astragalus adsurgens Pall.)
Erect milkvetch (Astragalus adsurgens Pall.) is a major legume forage plant widely grown in Northern China. However, the lack of molecular markers has limited its research into its genetic diversity and work on germplasm improvement. In this study, a total of 39,163 EST–SSR loci were identified from 30,262 unigene sequences in the erect milkvetch transcriptome using Illumina sequencing. Moreover, 22,367 EST–SSR primer pairs (PPs) were successfully designed. In addition, 100 PPs were synthesized and preliminarily screened in two accessions; of these, 90 were determined to be clear and stable EST–SSR markers. Fifty-one PPs were randomly selected in order to assess the genetic diversity of 27 erect milkvetch accessions. The average polymorphism information content of the 51 PPs was 0.682. Greater genetic diversity was detected in accessions from Inner Mongolia and in the group of landrace and wild erect milkvetch accessions. This study provides an important resource for germplasm improvement and genetic diversity analysis in erect milkvetch.
- 15 November 2018
- Published by Springer Nature in Molecular Biology Reports
- Vol. 46 (1) , 1323-1326
Development and application of EST–SSRs markers for analysis of genetic diversity in erect milkvetch (Astragalus adsurgens Pall.)
Erect milkvetch (Astragalus adsurgens Pall.) is a major legume forage plant widely grown in Northern China. However, the lack of molecular markers has limited its research into its genetic diversity and work on germplasm improvement. In this study, a total of 39,163 EST–SSR loci were identified from 30,262 unigene sequences in the erect milkvetch transcriptome using Illumina sequencing. Moreover, 22,367 EST–SSR primer pairs (PPs) were successfully designed. In addition, 100 PPs were synthesized and preliminarily screened in two accessions; of these, 90 were determined to be clear and stable EST–SSR markers. Fifty-one PPs were randomly selected in order to assess the genetic diversity of 27 erect milkvetch accessions. The average polymorphism information content of the 51 PPs was 0.682. Greater genetic diversity was detected in accessions from Inner Mongolia and in the group of landrace and wild erect milkvetch accessions. This study provides an important resource for germplasm improvement and genetic diversity analysis in erect milkvetch.