Nehad S. Alsubhi

Silver nanoparticles (AgNPs) are becoming increasingly important for various crucial applications, including antimicrobial, anticancer, catalytic, and anti-inflammatory. AgNPs biosynthesized from plant extracts have attracted considerable attention because of their eco-friendliness, simplicity, cost-effectiveness, and stability. This study investigated the potential of using fruit and leaf extracts of the medicinal plant Azadirachta indica as a capping and reducing agent for the biosynthesis of AgNPs. The size, shape, and optical properties of AgNPs significantly affect their chemical, physical, and biological activity. Therefore, this study optimized the biosynthesis conditions as a first attempt for A. indica fruit extracts to produce AgNPs with precise morphology. Subsequently, the biologically manufactured AgNPs were characterized using suitable techniques. Their potential anticancer activities were examined against in vitro human lung and breast cancer (H1975 and MCF-7) cell lines. The AgNPs were stable, with a high yield and a spherical shape, ranging in size from 14 to 19nm and exhibiting an absorption band between 420 and 440nm. The AgNPs biosynthesized using A. indica fruit and leaf extracts were shown to be highly toxic against in vitro H1975, with IC₅₀ of 62.2 and 91µg/mL, respectively. The IC₅₀ values were 67.5 and 68.7µg/mL when testing against the MCF-7 cells. These findings suggest that plant-derived nanoparticles have enormous potential for future biomedical applications, which warrants further investigation.

Green synthesis has attracted significant attention as an eco-friendly, low-cost, energy-efficient, and non-toxic method for preparing silver nanoparticles (AgNPs) for cancer therapy. This study optimized the green synthesis of AgNPs using Olea europaea extracts and evaluated their anticancer potential. The biosynthesized AgNPs were characterized using various methods, showing stable AgNPs with a desirable morphology and high yield, improving the properties of AgNPs for various medicinal applications. The biosynthesized AgNPs were predominantly spherical, with small sizes ranging from 13 to 21nm and highly stable at 23 and 24 mV. The findings of this study suggest that green-synthesized AgNPs using Olea europaea and sunlight possess significant anticancer activity against cancer cells in vitro. Further investigation of green synthesis would help to form high-quality AgNPs that have promising potential in treating disease and fighting undesirable pathogens.

Silver nanoparticles (AgNPs) have been considered promising candidates for medical practices in various fields. This study proposed an efficient, economical, uncomplicated, and reliable method to synthesize AgNPs utilizing leaf and fruit extracts of Juniperus procera (J. procera) as capping, reducing, and stabilizing agents. The study includes optimizing the green synthesis conditions to produce stable AgNPs with high yields, acceptable particle size, and shape, hence, AgNPs may be used for different medical purposes through the improvement of their properties. Several spectroscopic and other analyses performed characterization of the fabricated AgNPs, and the results show stable and spherical AgNPs between 14 and 18 nm in size. The study also evaluated the anticancer activities of the biosynthesized AgNPs using J. procera fruit and leaf extracts against in vitro lung cancer A549 and H1975 cells. The results demonstrate the high toxicity of the biosynthesized AgNPs against in vitro lung cancer cells, supporting therapeutic and biomedical applications of AgNPs.