Daranee Wasipraphai
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Daranee Wasipraphai has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Anchalee Homkaew
Microbiological Unit, Central Laboratory and Blood Bank, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand
1 shared publication - Huttaya Thuncharoon
Laboratory Department, Taksin Hospital, Bangkok 10600, Thailand
1 shared publication - Kinley Wangchuk
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
1 shared publication - Marea Jikka Nealiga
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
1 shared publication - Padungsri Dubbs
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
1 shared publication - Paveesuda Suksomchit
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
1 shared publication
Latest Publications
- Open Access
- 27 September 2022
- Published by MDPI AG in Microorganisms
- Vol. 10 (10) , 1917
Differential Interaction between Invasive Thai Group B Streptococcus Sequence Type 283 and Caco-2 Cells
The emergence in Southeast Asia of invasive group B Streptococcus (GBS) infections in adults by sequence type (ST) 283 is suggested to be associated with fish consumption. Genotyping of 55 GBS clinical isolates revealed that 33/44 invasive isolates belonged to ST283/capsular polysaccharide type (CPS) III. This included 15/16 isolates recovered from younger adults aged 16–36 years. Seven ST283/CPSIII isolates from the blood, cerebrospinal fluid, or joint fluid were selected by the patient’s age at random to perform interaction studies with intestinal epithelial Caco-2 monolayers. The invasion efficiency profiles from this study classified these isolates into two groups; a higher invasion efficiency group 1 recovered from patients aged between 23 and 36 years, and a lower invasion efficiency group 2 recovered from the elderly and neonate. Intracellular survival tests revealed that only group 1 members could survive inside Caco-2 cells up to 32 h without replication. Additionally, all isolates tested were able to traverse across polarized Caco-2 monolayers. However, the timing of translocation varied among the isolates. These results indicated the potential of GBS invasion via the gastrointestinal tract and showed phenotypic variations in invasiveness, intracellular survival, and translocation efficiency between genetically closely related ST283 isolates infecting young adults and those infecting the elderly.
- 27 September 2022
- Published by MDPI AG in Microorganisms
- Vol. 10 (10) , 1917
Open AccessDifferential Interaction between Invasive Thai Group B Streptococcus Sequence Type 283 and Caco-2 Cells
The emergence in Southeast Asia of invasive group B Streptococcus (GBS) infections in adults by sequence type (ST) 283 is suggested to be associated with fish consumption. Genotyping of 55 GBS clinical isolates revealed that 33/44 invasive isolates belonged to ST283/capsular polysaccharide type (CPS) III. This included 15/16 isolates recovered from younger adults aged 16–36 years. Seven ST283/CPSIII isolates from the blood, cerebrospinal fluid, or joint fluid were selected by the patient’s age at random to perform interaction studies with intestinal epithelial Caco-2 monolayers. The invasion efficiency profiles from this study classified these isolates into two groups; a higher invasion efficiency group 1 recovered from patients aged between 23 and 36 years, and a lower invasion efficiency group 2 recovered from the elderly and neonate. Intracellular survival tests revealed that only group 1 members could survive inside Caco-2 cells up to 32 h without replication. Additionally, all isolates tested were able to traverse across polarized Caco-2 monolayers. However, the timing of translocation varied among the isolates. These results indicated the potential of GBS invasion via the gastrointestinal tract and showed phenotypic variations in invasiveness, intracellular survival, and translocation efficiency between genetically closely related ST283 isolates infecting young adults and those infecting the elderly.