Nian Wei

The Geheyan Reservoir, located on the Qingjiang River, a tributary of the Yangtze River, is important for regional water supplies and ecological conservation. Understanding changes in ecosystem structure and function has become critical for assessing efficacy after the implementation of a fishing ban. This study employs the Ecopath model to examine the ecosystem characteristics of the Geheyan Reservoir before (2017) and after (2022) the fishing ban. The results show significant differences in trophic levels, energy transfer efficiency, and ecosystem maturity between the two periods. The trophic levels increased from 3.36 pre-fishing ban to 3.89 post-ban, indicating an enhanced complexity in the food web structure. The highest eco-trophic efficiency for major commercial fish species increased after the ban, indicating improved energy utilization efficiency. However, energy transfer bottlenecks were still observed between trophic levels II and IV, suggesting ongoing challenges in nutrient cycling. The total primary production-to-total respiration ratio (6.93) and the connectivity index (0.25) indicate that the ecosystem’s maturity and stability have improved after the fishing ban. These findings underscore the ban’s effectiveness and provide a scientific foundation for sustainable management of Geheyan Reservoir and similar ecosystems in the Yangtze River Basin.

This study evaluated the habitat coloration preferences of Siniperca chuatsi and Cirrhinus molitorella in both solitary (n = 1) and group (n = 3) settings across six colors. The results indicated that both individual and group S. chuatsi spent the majority of their time in and made frequent visits to the black area, followed by the blue area. While individual C. molitorella spent the majority of their time and visits in the blue region, groups showed a preference for the blue and white regions. These findings highlight the distinct habitat coloration preferences of S. chuatsi and C. molitorella in different group states, suggesting that habitat coloration has an important effect on fish behavior and environmental adaptation. From a behavioral ecology perspective, these preferences may be closely related to the survival strategies of fish. Notably, C. molitorella’s strong preference for blue and white backgrounds may reflect anti-predator behavior, helping C. molitorella avoid predators, such as S. chuatsi, in natural environments, thereby improving its chances of survival. This study provides a scientific basis for optimizing aquaculture environments, emphasizing the importance of considering habitat coloration and substrate type in designing environments to enhance fish welfare.

One of the crucial factors influencing the growth and viability of larvae and juveniles is their opening diets. The objective of this study was to identify suitable initial feed options for M. macropterus larvae and juveniles. A total of 1200 newly hatched M. macropterus with an average weight of 18.3 mg and an average length of 11.58 mm were selected and randomly divided into four groups. The fish were fed with different opening diets, including rotifer, Artemia nauplii, Tubifex, and micro-diet from six days after hatching (dahs), respectively. Growth indices and activities of digestive enzymes were assessed at 10, 15, 20, 25, 30, 35, and 40 dahs. Histological examination of the structure of the digestive tract was performed at 40 dahs, while survival rates were also documented. The results demonstrated that different diets had no effect on the survival rate of larvae and juveniles of M. macropterus. The growth performance indices were ranked as follows: Tubifex group > Artemia nauplii group > micro-diet group > rotifer group. Remarkably, the Tubifex group exhibited superior growth performance, which was also reflected in the structure of the digestive tract and digestive enzyme activity. Therefore, it is recommended to include Tubifex in the diet of M. macropterus larvae and juvenile during the standardized farming process.

Cadmium (Cd²⁺) and mercury (Hg²⁺) are two kinds of non-essential heavy metals. Cd²⁺ and Hg²⁺ can cause thyroid disruption, but very few researchers have investigated the thyroid-disrupting effects of these metals on fish, specifically during their early developmental transition stage from embryos to larvae. In this study, wild-type zebrafish embryos were exposed to varying concentrations (contents) of Cd²⁺ (0, 10, 100, and 1000 μg/L) and Hg²⁺ (0, 0.1, 1, and 10 μg/L) for 120 h. Thereafter, the thyroid hormone contents and transcriptional changes in the genes, including thyroid stimulating hormone-β (tshβ), thyroglobulin (tg), sodium-iodide symporter (nis), thyroid peroxidase (tpo), transthyretin (ttr), thyroid hormone receptor-α and -β (thrα, thrβ), types I and II iodothyronine deiodinase (dio1, dio2), and uridine diphosphate glucuronosyltransferase 1 family a, b (ugt1ab) associated with the hypothalamic-pituitary-thyroid (HPT) axis were measured. Results showed that zebrafish embryos/larvae malformation rates were significantly higher in the Cd²⁺ and Hg²⁺ groups. A significant increase in the thyroxine (T4) concentration and a decrease in the triiodothyronine (T3) concentration were observed in the Cd²⁺-exposed zebrafish embryos/larvae. On the other hand, the T4 and T3 concentrations were observed to be significantly increased after Hg²⁺ exposure. Additionally, changes were noted in the expression patterns of the HPT axis-linked genes after Cd²⁺ and Hg²⁺ exposure. Based on the results of the principal component analysis (PCA), it was concluded that Cd²⁺ exposure significantly affected the thyroid endocrine system at a concentration of 100 μg/L, whereas Hg²⁺ exposure led to a thyroid disruption at a low concentration of 0.1 μg/L. Thus, this study demonstrated that exposure to Cd²⁺ and Hg²⁺ metal ions induced developmental toxicity and led to thyroid disruption in zebrafish embryos/larvae.

Copper (Cu²⁺) and zinc (Zn²⁺) are two kinds of heavy metals essential to living organisms. Cu²⁺ and Zn²⁺ at excessive concentrations can cause adverse effects on animals, but little is known about the thyroid-disrupting effects of these metals in fish, especially in the early developmental transition stage from embryos to larvae. Wild-type zebrafish embryos were used to expose to Cu²⁺ (0, 1.5, 15, and 150 μg/L) and Zn²⁺ (0, 20, 200, and 2000 μg/L) for 120 h. Thyroid hormone contents and transcriptional changes of the genes connected with the hypothalamic-pituitary-thyroid (HPT) axis were measured. Results showed that zebrafish embryos/larvae malformation rates were significantly increased in the Cu²⁺ and Zn²⁺ groups. Remarkably elevated thyroxine (T4) concentrations and reduced triiodothyronine (T3) concentrations were observed in Cu²⁺ and Zn²⁺ exposure fish. And the expression patterns of genes connected with the HPT axis were changed after Cu²⁺ and Zn²⁺ treatment. Based on principal component analysis (PCA) results, Zn²⁺ caused significant effects on the thyroid endocrine system at 200 μg/L, while Cu²⁺ resulted in thyroid disruption as low as 1.5 μg/L. In short, our study demonstrated that exposure to Cu²⁺ and Zn²⁺ induced developmental toxicity and thyroid disruption to zebrafish embryos/larvae.

Sulfide is considered as an environmental factor and toxicant with a wide distribution in aquatic environments. At present, the toxic effects of sulfide stress on rice field eel (Monopterus albus) are poorly understood. To ascertain these effects, the juvenile rice field eels were exposed to sub-lethal concentrations of Na₂S (0, 0.2154, 2.154, and 21.54 mg/L) for 7, 14, and 28 days. Antioxidant parameters such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) as well as the related gene (including sod, cat) expressions were measured. The expressions of metallothionein (mt) and heat shock protein 90 (hsp90), which are used as physiological stress indices, were also detected. The results showed that exposure to sulfide altered the antioxidant response and related gene expressions in rice field eel. The activities of SOD were inhibited and the MDA contents were increased after exposure to sulfide. The activities of CAT and GPx were increased at 7 days and decreased at 28 days after treatment with sulfide at the highest dose. The expressions of sod, cat, and hsp90 were upregulated at 7 days and downregulated at 14 and 28 days after exposure to high doses of sulfide. The expression of mt was significantly downregulated in all sulfide treated groups. The toxic effects caused by sulfide were in dose-dependent and time-dependent manners. In short, oxidative stress and physiological stress were caused in rice field eel after the exposure to sulfide.