Jian Wang

Nicosulfuron (NIF) stress poses a significant constraint on sweet corn production. Graphene oxide (GO), as an environmentally friendly nanomaterial, serves as a safe agent to enhance crop stress resistance. However, the molecular mechanisms of GO-mediated sweet corn to tolerate NIF remain largely unexplored. This study aimed to investigate the regulatory effects of GO on sweet corn's response to NIF through physiological assessments, comparative transcriptomic analysis, and metabolomic profiling. The findings indicate that the application of GO mitigates the detrimental effects of NIF stress on sweet corn seedlings, as evidenced by enhanced growth metrics, improved chlorophyll fluorescence parameters, and photosynthetic indicators. GO predominantly influences genes and metabolites related to the synthesis of flavonoids, phenylpropanoids, and calcium (Ca²⁺) signaling pathways to counteract NIF stress in sweet corn. Additionally, key genes associated with peroxidases (POD) and cytochrome P450 monooxygenases (CYP) were identified through the analysis of weighted gene co-expression networks (WGCNA). These genes play crucial roles in the response to NIF stress orchestrated by graphene oxide. In summary, this study demonstrates the synergistic regulatory effect of GO on NIF tolerance in sweet corn, providing valuable insights for developing NIF-resistant sweet corn varieties.

To clarify the effectiveness of nicosulfuron (NIF) in controlling weeds in cornfields and the physiological response mechanisms of corn, this study synthesized 29 relevant articles for a meta-analysis. A pair of sweet corn sister lines with different resistance to NIF (resistant HK301 and sensitive HK320) was selected as materials, and the responses of non-target metabolic pathways under NIF stress were analyzed using structural equation modeling. The results indicate that NIF is highly effective against weeds such as Eleusine indica and Amaranthus retroflexus, but less effective against weeds such as Cyperus difformis (13.20%) and Abutilon theophrasti (−4.10%). High concentrations (≥60 g hm−2) and prolonged application (more than 30 days) significantly enhance effectiveness (up to 170.60% and 155.50%, respectively). In terms of physiological mechanisms, the antioxidant capacity, photosynthetic performance and levels of non-enzymatic antioxidants in sensitive maize (Maize.G) were significantly lower than those in the resistant Maize.K, with higher accumulation of ROS and MDA. The structural equation model revealed that in HK301, NIF induced oxidative stress effectively activates the antioxidant defense system, inhibits ROS accumulation and membrane lipid peroxidation, reduces photosynthetic damage, and maintains physiological homeostasis. Conversely, in HK320, excessive oxidative stress leads to impaired antioxidant function and a decline in photosynthetic performance. This study demonstrates physiological differences in NIF responses between resistant and sensitive maize lines, providing practical guidance for the rational use of NIF in corn-fields and, theoretical insights into the non-target effects of herbicides and crop resistance mechanisms.

Breeding and cultivating low-N-efficient maize varieties to obtain high yields with less N fertilizer is important for addressing food demands and environmental pollution. However, few studies have investigated the physiological characteristics of low-N-efficient maize varieties. Therefore, we performed an experiment over four years to test two maize varieties (low-N-efficient variety: JNK728, and high-N-efficient variety: XY335) and five N application rates (N120: 120 kg·ha⁻¹, N180: 180 kg·ha⁻¹, N240: 240 kg·ha⁻¹, N300: 300 kg·ha⁻¹, and N360: 360 kg·ha⁻¹). The optimal N application rates for JNK728 and XY335 were N180 and N300, which obtained the highest yields (11,754 and 12,752 kg·ha⁻¹, respectively), N uptake efficiencies (1.32 and 0.93 kg·kg⁻¹), and N harvest index (67.94% and 61.98%), compared with other N application rates. The key period for plant N accumulation was the R1–R6 stage, which contributed 35.2–49.7% and 40.8–53.8% to plant N accumulation at the maturation stage in JNK728 and XY335, respectively. In addition, N accumulation in the grain accounted for more than half (51.8–63.2%) of the total N accumulation in plants, and the leaf N transport amount after the post-silking stage was the primary source of grain N accumulation in both JNK728 and XY335. We also explored the key enzymes and genes related to the N transport amount and efficiency in leaves in the two maize varieties, and found that GOGAT was the key enzyme and GOGAT2 was the key gene for JNK728, whereas the AS enzyme and AS1 and AS3 genes were most important for XY335. Therefore, we suggest that molecular breeding programs should focus on the GOGAT2 gene in low-N-efficient maize varieties, and cultivation techniques should aim to improve the GOGAT enzyme activity after the post-silking period to achieve high yields and N utilization efficiencies with less N fertilizer.

No-tillage of summer maize after the harvest of winter wheat is the primary agricultural practice on the North China Plain. However, prolonged no-tillage without deep tillage practices negatively impacts soil properties, which is detrimental to the growth and yield of summer maize. In this study, Xianyu 688 and Jifeng 2 were used as test maize materials, no-tillage and surface fertilizing with normal nitrogen (N) (BC240), no-tillage and surface fertilizing with N reduction (BC180), subsoiling layered fertilization with normal N (FC240) and subsoiling layered fertilization with N reduction (FC180)were designed, in order to assess root distribution, N utilization and grain yield of summer maize. In the two maize cultivars, the FC240 and FC180 treatments significantly reduced soil bulk density in the 10–50 cm depth soil layer compared to the other two treatments, and also increased total N content in the 20–50 cm depth soil layer. Compare BC 240 treatment, the FC240 treatment significantly increased root length in the 20–40 cm soil layer and root rap bleeding. Additionally, the FC240 and FC180 treatments enhanced dry matter and N accumulation, grain yield, N uptake efficiency and N fertilizer partial factor productivity. In various treatment, Xianyu 688 exhibited increased grain yield, N uptake efficiency and N fertilizer partial factor productivity compare with Jifeng 2. When employing a total N application level of 180–240 kg N/hm², the synergistic improvement of summer maize grain yield and N efficiency can be achieved by incorporating subsoiling combined with layered nitrogen application.

Starch is an important reserve of sugar, and starch-sugar conversion in plants plays an important role in the response of plants to various abiotic stresses. Nicosulfuron is a post-emergence herbicide commonly applied to maize fields. However, it is unclear how sucrose and starch in sweet corn are converted to accommodate nicosulfuron stress. Field and pot experiments were conducted to study the effects of nicosulfuron on the sugar metabolism enzymes, starch metabolism enzymes, non-enzyme substances, and expression of key enzyme genes in leaves and roots of sweet maize seedlings. Accordingly, this research compared the responses of the sister lines HK301 and HK320, which are nicosulfuron tolerant and sensitive, respectively. Under nicosulfuron stress, compared with HK301 seedlings, the accumulation of stem and root dry matter of HK320 seedlings was significantly reduced, resulting in a lower root-to-shoot ratio. Compared with HK320 seedlings, nicosulfuron stress significantly increased the sucrose, soluble sugar, and starch contents in HK301 leaves and roots. This may be related to the enhanced carbohydrate metabolism under nicosulfuron stress, including significant changes in sugar metabolism enzyme activity and the levels of SPS and SuSys expression. Further, under nicosulfuron stress, sucrose transporter genes (SUC 1, SUC 2, SWEET 13a, and SWEET 13b) in the leaves and roots of HK301 seedlings were significantly upregulated. Our results emphasize that changes in sugar distribution, metabolism, and transport can improve the adaptability of sweet maize to nicosulfuron stress.

Root morphology is an important factor determining nitrogen (N) uptake by plants, which might be affected by the extent of N application. The processes associated with root morphogenesis of spring maize in response to N application rates remain poorly understood. In this study, both field and pot experiments were conducted to explore the effect of zero-N (N0), optimized-N (N180), and high-N (N360) on root morphology, anatomical structure, and N accumulation in spring maize. N application rates affected root length and surface area, and its endogenous hormone contents. The largest difference in total root length and surface area among the three N rates was found at the silking stage: the total root length and surface increased by 51.36% and 42.58% under N180 and by 7.8% and 30.14% under N360, respectively, compared with N0, and the root/shoot ratio and root bleeding sap significantly increased under N180 and N360 compared with N0. The auxin and jasmonic acid levels of roots under N180 and N360 were higher than N0. N application rates also affected root microstructure and ultrastructure. Compared with N0, the proportions of root aerating tissue under N180 and N360 were decreased by 32.42% and 11.92% at silking. The root tip cell structure was damaged under N0, and intact under N180 and N360. Moreover, the ¹⁵N allocation proportions to root and grain under N180 and N360 were increased compared to N0. Grain yields under N180 and N360 increased by 20.44% and 16.6% compared with N0, respectively. It can be concluded that optimized-N application decreased root aerated tissue and thus improved root length and root surface area through regulating auxin and jasmonic acid levels and affected N uptake and grain yield of N-efficient spring maize variety.

The sulfonylurea herbicide nicosulfuron is efficient, harmless and selective at low doses and has been widely used in maize cultivation. In this study, a pair of corn sister lines, HK301 (nicosulfuron-tolerence, NT) and HK320 (nicosulfuron-sensitive, NS), was chosen to study the effect of nicosulfuron on plant growth and sugar metabolism in sweet maize (Zea mays L.) seedlings. All the experimental samples were subjected to treatment with water or 80 mg kg^–1 of nicosulfuron when the sweet maize seedlings grew to the four-leaf stage. Nicosulfuron significantly inhibited the growth of NS line. The content of sucrose and the activities of sucrose phosphate synthase and sucrose synthase in the two inbred lines increased differentially under nicosulfuron stress compared with the respective control treatment. After nicosulfuron treatment, the activities of hexokinase and 6-phosphofructokinase and the contents of pyruvic acid and citric acid in NS line decreased significantly compared with those of NT line, while the content of sucrose and activities of sucrose phosphate synthase and sucrose synthase increased significantly. The disruption of sugar metabolism in NS line led to a lower supply of energy for growth. This study showed that the glycolysis pathway and the tricarboxylic acid cycle were enhanced in nicosulfuron-tolerant line under nicosulfuron stress in enhancing the adaptability of sweet maize.

Weed control in maize (Zea mays L.) crops is usually undertaken using the postemergence herbicide nicosulfuron. The toxicity of nicosulfuron on maize, especially sweet maize, has been widely reported. In order to examine the effect of nicosulfuron on seedling photosynthetic characteristics, chlorophyll fluorescence, reactive oxygen species production, antioxidant enzyme activities, and gene expressions on sweet maize, nicosulfuron-tolerant “HK310” and nicosulfuron-sensitive “HK320” were studied. All experiment samples were subjected to a water or 80 mg kg⁻¹ of nicosulfuron treatment when sweet maize seedlings grow to the stage of four leaves. After treatment with nicosulfuron, results for HK301 were significantly higher than those for HK320 for net photosynthetic rate, transpiration rate, stomatal conductance, leaf maximum photochemical efficiency of PSII, photochemical quenching of chlorophyll fluorescence, and the electron transport rate. These results were contrary to nonphotochemical quenching and intercellular CO₂ concentration. As exposure time increased, associated effects also increased. Both O₂^·− and H₂O₂ detoxification is modulated by antioxidant enzymes. Compared to HK301, SOD, POD, and CAT activities of HK320 were significantly reduced as exposure time increase. Compared to HK320, the gene expression for the majority of SOD genes, except for SOD2, increased due to inducement by nicosulfuron, and it significantly upregulated the gene expression of CAT in HK301. Results from this study indicate that plants can improve photosynthesis, scavenging capabilities of ROS, and protective mechanisms to alleviate phytotoxic effect of nicosulfuron. Future research is needed to further elucidate the important role antioxidant systems and gene regulation play in herbicide detoxification in sweet maize.

Effects of nitrogen fertilizer on root-shoot characteristics, grain yield and nitrogen use efficiency of different maize varieties in North China Plain