Michael Karski
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Michael Karski has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Biju Issac
Research Computing, Information Technology, Boston Children’s Hospital, Boston, MA 02472, USA
1 shared publication - Khashayar Vakili
Department of Surgery, Boston Children’s Hospital, Boston, USA
1 shared publication - Liang Sun
Research Informatics, Boston Children’s Hospital, Boston, MA, USA
1 shared publication - Piotr Sliz
Children's Rare Disease Collaborative, Boston Children's Hospital, Boston, MA, USA; Department of Pediatrics, Boston Children's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; The Manton Center for Orphan Disease Research, Boston Children's Hospital, Boston, MA, USA; Division of Molecular Medicine, Boston Children's Hospital, Boston, MA, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
1 shared publication - Ranan Gulhan Aktas
Department of Surgery, Boston Children’s Hospital, Boston, MA 02472, USA
1 shared publication - Shira Rockowitz
Research Informatics, Department of Information Technology, Boston Children’s Hospital, Boston, Massachusetts, USA
1 shared publication
Latest Publications
- Open Access
Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition
Organoids have been used to investigate the three-dimensional (3D) organization and function of their respective organs. These self-organizing 3D structures offer a distinct advantage over traditional two-dimensional (2D) culture techniques by creating a more physiologically relevant milieu to study complex biological systems. The goal of this study was to determine the feasibility of establishing organoids from various pediatric liver diseases and characterize the long-term evolution of cholangiocyte organoids (chol-orgs) under a single continuous culture condition. We established chol-orgs from 10 different liver conditions and characterized their multicellular organization into complex epithelial structures through budding, merging, and lumen formation. Immunofluorescent staining, electron microscopy, and single-nucleus RNA (snRNA-seq) sequencing confirmed the cholangiocytic nature of the chol-orgs. There were significant cell population differences in the transcript profiles of two-dimensional and organoid cultures based on snRNA-seq. Our study provides an approach for the generation and long-term maintenance of chol-orgs from various pediatric liver diseases under a single continuous culture condition.
Open AccessLong-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition
Organoids have been used to investigate the three-dimensional (3D) organization and function of their respective organs. These self-organizing 3D structures offer a distinct advantage over traditional two-dimensional (2D) culture techniques by creating a more physiologically relevant milieu to study complex biological systems. The goal of this study was to determine the feasibility of establishing organoids from various pediatric liver diseases and characterize the long-term evolution of cholangiocyte organoids (chol-orgs) under a single continuous culture condition. We established chol-orgs from 10 different liver conditions and characterized their multicellular organization into complex epithelial structures through budding, merging, and lumen formation. Immunofluorescent staining, electron microscopy, and single-nucleus RNA (snRNA-seq) sequencing confirmed the cholangiocytic nature of the chol-orgs. There were significant cell population differences in the transcript profiles of two-dimensional and organoid cultures based on snRNA-seq. Our study provides an approach for the generation and long-term maintenance of chol-orgs from various pediatric liver diseases under a single continuous culture condition.