Farida Juliantina Rachmawaty

Background Treatment of tuberculosis is still a problem in Indonesia or in the world. There needs to be a new alternative drug for tuberculosis therapy. Objective The purpose of this study was to isolate and identify the active compounds from ethanol extract of red betel vine (Piper crocatum) leaves as an antituberculosis candidate. Methods The chloroform fraction of 70% ethanol extract was further purified, tested antimycobacterial activity against M. tuberculosis and identified its chemical structure using the method of Infra Red (FTIR) Spectrophotometer, UV-Vis Spectrophotometer and H/C-Nuclear Magnetic Resonance Spectrometer. Results Chloroform fraction identified 29 compounds.Compound 1 (S1) with Rf 0.96 has the best activity against M. tuberculosis with a minimum bactericidal concentration (MBC) of 5 μg/mL. The analysis of FTIR spectra and UV-Vis spectra, it is proposed that S1 compound is a triterpenoid compound which is hydroxylated and has more than one aliphatic alkene group. Conclusion The isolated compound with the best antimycobacterial activity against M. tuberculosis is suggested to be a hydroxylated triterpenoid containing multiple aliphatic alkene groups. BJMS, Vol. 24 No. 03 July’25 Page : 778-784

Pernikahan bukan hanya sebagai pelengkap separuh agama, salah satu sunnah rasul yang hendaknya dilakukan umatnya, tetapi juga untuk mewujudkan generasi unggul baik budi maupun pekertinya. Individu yang akan menikah, hendaknya memiliki bekal ilmu pengetahuan demi terwujudnya keluraga yang harmonis, Sakinah mawaddah warohmah (Keluarga Samara). Namun, pada realitanya kebanyakan individu disibukkan dengan mengejar karier demi memenuhi tuntutan hidup, tapi disisi lain dan sangat minim mempersiapkan diri untuk mewujudkan sebuah keluarga. Alhasil keluarga dibentuk hanya berdasar pengalaman yang diterima tanpa tahu benar salahnya atau manakah yang ideal. Akibatnya banyak kita jumpai permasalahan keluarga seperti perceraian, perseteruan keluarga, kenakalan remaja, penggunaan narkoba dan lain-lain yang sering membuat hati miris. Hal tersebut masih banyak terjadi di Indonesia. Padahal Allah berfirman, “…Peliharalah dirimu dan keluargamu dari api neraka …” (Q.S. At Tahrim: 6). Dilatarbelakangi hal tersebut, kegiatan School of Parenting Keluarga Samara (“Mewujudkan Keluarga Sakinah Membentuk Generasi Unggul”) dimaksudkan untuk memberi dasar awal pada keluarga muda atau calon orangtua mewujudkan fondasi rumah tangganya agar sakinah mawadah warohmah dan terbentuk generasi unggul sesuai yang diharapkan. Kata Kunci: keluarga Samara, permasalahan keluarga, school of parenting.

Background: The red betel vine (Piper crocatum) is a plant that has potential as a medicinal plant. The use of medicinal plants should be tested for safety. Acute toxicity testing has been performed with safe results for consumption, but there is no known long-term effect on various organs.Objective: To determine whether an ethanol extract of the red betel vine could be used safely on different parts of DDY mice.Methods: The treatment of ethanol extract was performed orally every day by using a feeding tube with different doses in mice within 3 months. Those were divided into several groups, each consisting of 5 male and 5 female mice. The dosages used consisted of 50 mg/kgBW, 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW, with aquadest and solvent as controls. The treatment was done every day for 3 months. At the end of the study, the mice were terminated, and their organs were collected, which included the spleen, liver, kidney, heart, lungs, brain, testes, and ovaries.Results: The results of this study show no significant difference compared to controls in all organs. There are liver changes from cloudy swelling degeneration and reversible hydropic degeneration, but the effect is reversible.Conclusion: To conclude, long-term use of the red betel vine as a medicinal plant can be recommended for healthy people but not for people with a history of hepatic disorders.International Journal of Human and Health Sciences Vol. 08 No. 01 Jan’24 Page: 69-74

Nutritional status is a condition that reflects the balance between nutrient intake and nutrient needs for body metabolism. Malnutrition is still a priority for national health development. Malnutrition in children causes growth and development problems and affects the quality of resources. The risk of malnutrition can occur to the students at the Islamic boarding school because they do not live and are cared for by their parents. Therefore, this community service focuses on screening and anticipating calorie deficiency in elementary school students. Ash Sholihah Islamic Boarding School is a boarding school in Sleman Regency with simple facilities. The number of boarding school students is approximately 700 male and female students, consisting of elementary to high school students. Elementary school students need more attention because they cannot take care of themselves and maintain food intake properly. The results of the anthropometric examination of 198 children showed that 166 (83.6%) were underweight, 24 (12.1%) had ideal body weight, and 8 (4.04%) were overweight. The results of the anthropometric examination of 198 children showed that 166 (83.6%) were underweight, 24 (12.1%) had ideal body weight, and 8 (4.04%) were overweight. The results of the mini nutritional assessment (MNA) questionnaire screening showed 10 (5.1%) malnutrition, 165 (83.3%) risk of malnutrition, 8 (4%) good nutrition, and 15 (7.6%) unknown. The service activities were continued with medical examinations and administration of drugs if necessary. Then carried out education and provision of additional calories to 166 children with underweight. Dormitory administrators are involved in supervising the implementation of additional nutrition. Additional intake given is a preparation equivalent to 100 kcal/day for each child as modified Formula 100.

This research aims to identify the antimycobacterial activity of fraction of red betel vine leaves ethanol extract (methanol fraction, ethyl acetate, and chloroform) toward M. tuberculosis. Red betel vine leaves ethanol extract was made with maceration method using ethanol solvent 70%. Resulted extract was then fractionated using Liquid Vacuum Chromatography (LVC) with methanol, ethyl acetate, and chloroform solvent. Each fractionation was exposed to M. tuberculosis with serial dilution method. Controls of fraction, media, bacteria, and isoniazid as standard drug were included in this research. The group of compound from the most active fraction was then identified. The research found that the best fraction for antimycobacterial activity toward M. tuberculosisis chloroform fraction. The compound group of chloroform fraction was then identified. The fraction contains flavonoid, tannin, alkaloid, and terpenoid. The fraction of methanol, ethyl acetate, and chloroform from red betel vine leaves has antimycobacterial activity toward M. tuberculosis. Chloroform fraction has the best antimycobacterial activity and it contains flavonoid, tannin, alkaloid, and terpenoid.