Thi Thuy Minh Nguyen

The deterioration of dams and levees is an increasing concern for both infrastructure integrity and environmental sustainability. The extensive repercussions, including the displacement of communities, underscore the imperative for sustainable interventions. This study addresses these challenges by investigating the stabilization of dredged material (DM) for diverse applications. Seven mixtures incorporating fly ash, lime, and cement were formulated. The Standard Compaction Test was used to determine optimal density–moisture conditions, which helped with brick fabrication. Bricks were tested for compressive strength over various curing periods, and the durability of the 28-day-cured samples was evaluated by performing water immersion tests following the New Mexico Code specifications. Scanning electron microscopy (SEM) was used to assess microstructural bonding. Results confirm that the inclusion of cementitious stabilizers modifies the material’s microstructure, resulting in enhancements of both strength and water resistance. Notably, the stabilized material demonstrates potential for use in non-fired brick manufacturing and as bridge stones for waterway erosion control. This dual-function application offers a sustainable and economically feasible approach to managing dredged materials.

Sediments are dredged from waterways to maintain maritime activities and prevent floods. Exorbitant amounts of money are budgeted for the removal of dredged material (DM) and its disposal in landfills. We investigated the potential for reuse of DM as a road construction material using so-called bio-enzyme products as stabilizing agents. To improve the mechanical properties of DM, such as compressive strength, compressibility, Atterberg limits and the California bearing ratio (CBR), mixtures of DM were tested with two different amounts of a commercially available bio-enzyme product, which yielded enzymatically stabilized dredged material (ESDM). Unconfined compressive strength (UCS), compaction and Atterberg limits were measured in accordance with ASTM specifications on all samples. Data show that the addition of bio-enzymes resulted in increases in UCS but did not affect the optimum moisture content (OMC), maximum dry unit weight or Atterberg limits of the DM. A comparative field study was carried out to evaluate the CBR of the CH subgrade before and after treatments with the bio-enzyme product and with lime as a traditional stabilizing agent. The results of the field study supported the laboratory findings. Based on these data and results from the literature, models predicting the effect of bio-enzyme treatments on the value of CBR and of UCS were developed statistically. These models also underlined the importance of the clay fraction and PI values for the improvement of the engineering properties of soil using bio-enzyme additives.

To maintain adequate depth of commercial waterways, large quantities of earthen material are dredged and stored on undeveloped placement areas adjacent to the waterway. As dredge placement areas become overwhelmed, an environmental and financial sustainable solution for the reuse of dredged soil is prioritized. In this study, locally dredged material from the Sabine-Neches Waterway was used to explore the potential of dredged material in the production of compressed stabilized earth bricks (CSEBs) for small-scale structures in the region. CSEB mixture designs were developed containing fly ash (FA), Portland cement (PC), hydrated lime (HL), water (W), dredged material (DM), and natural and synthetic fibers. Optimized mixtures designs reached the recommended compressive strength of over 1200 psi. Results showed that that the addition of fibers reduced the compressive and flexural strength of the bricks, with a maximum compressive strength of 1394 psi with a corresponding flexural strength of 381 psi being obtained with fiberless dredge bricks. Multiple coating systems were also tested to increase the resistance of the bricks to weathering and erosion. Results showed that the use of coatings reduced water absorption and increased the bricks resistance to erosion, making them more adept in regions commonly subjected to flooding and heavy wind-driven rains.

Zinc Oxide (ZnO) nanoparticles are suspected to produce toxic effects toward mammalian cells; however, discrepancies in the extent of this effect have been reported between different cell lines. Simultaneously, high levels of ultraviolet (UV-C) radiation can have carcinogenic effects. The mechanism of this effect is also not well understood. Due to similarities in phenotype morphology after cell exposure to ZnO nanoparticles and UV-C irradiation, we emit the hypothesis that the toxicity of both these factors is related to damage of cellular membranes and affect their sterol content. Wild-type Chinese Hamster Ovary (CHO-K1) cells were exposed to ZnO nanoparticles or UV-C radiation. The amount of absorbed ZnO was determined by UV-visible spectroscopy and the changes in sterol profiles were evaluated by gas chromatography. Cell viability after both treatments was determined by microscopy. Comparing morphology results suggested similarities in toxicology events induced by ZnO nanoparticles and UV exposure. UV-C exposure for 360 min disrupts the sterol metabolic pathway by increasing the concentration of cholesterol by 21.6-fold. This increase in cholesterol production supports the hypothesis that UV irradiation has direct consequences in initiating sterol modifications in the cell membrane.

The tightly coupled nature of the electrophilic alkylation reaction sequence catalysed by 24-SMT (sterol C-24-methyltransferase) of land plants and algae can be distinguished by the formation of cationic intermediates that yield phyla-specific product profiles. C-24-methylation of the cycloartenol substrate by the recombinant Glycine max (soybean) 24-SMT proceeds to a single product 24(28)-methylenecycloartanol, whereas the 24-SMT from green algae converts cycloartenol into two products cyclolaudenol [∆(25(27))-olefin] and 24(28)-methylenecycloartanol [(∆24(28))-olefin]. Substrate analogues that differed in the steric-electronic features at either end of the molecule, 26-homocycloartenol or 3β-fluorolanostadiene, were converted by G. max SMT into a single 24(28)-methylene product. Alternatively, incubation of the allylic 26-fluoro cyclosteroid with G. max SMT afforded a bound intermediate that converted in favour of the ∆(25(27))-olefin product via the cyclolaudenol cation formed initially during the C-24-methylation reaction. A portion of the 26-fluorocycloartenol substrate was also intercepted by the enzyme and the corresponding hydrolysis product identified by GC-MS as 26-fluoro-25-hydroxy-24-methylcycloartanol. Finally, the 26-fluorocycloartenols are competitive inhibitors for the methylation of cycloartenol and 26-monofluorocycloartenol generated timedependent inactivation kinetics exhibiting a kinact value of 0.12 min(-1). The ability of soybean 24-SMT to generate a 25-hydroxy alkylated sterol and fluorinated ∆(25(27))-olefins is consistent with our hypothesis that (i) achieving the cyclolaudenyl cation intermediate by electrophilic alkylation of cycloartenol is significant to the overall reaction rate, and (ii) the evolution of variant sterol C-24-methylation patterns is driven by competing reaction channels that have switched in algae from formation of primarily ∆(25(27)) products that convert into ergosterol to, in land plants, formation of ∆(24(28)) products that convert into sitosterol.