Barbara A. Block

The capacity to monitor shark biodiversity is vital for conservation. Baited Remote Underwater Video Systems (BRUVS) are an increasingly popular and cost-effective method to characterize their biodiversity, abundance, and distribution. However, BRUV monitoring remains hindered by extensive manual video annotation and a lack of accessible tools to generate datasets for ecological analysis.To overcome these barriers, we developed a suite of automatic tools for shark detection and taxonomic classification. We integrated an object detection model (SharkTrack) with hierarchical species classification (Shark Detector) trained on 264,712 images of 309 shark species, in two open platforms—an R package (sharkDetectoR) and a lightweight desktop application (SharkByte)—making the updated model package an accessible tool across computational environments and user expertise.Tested on 46,332 holdout images and 13.9 hours of BRUV footage from the Main Hawaiian Islands and Palau, these tools achieved 92% species-level accuracy as well as 94.7% detection accuracy, and reduced annotation time by up to 90%.These findings underscore the value of combining scalable artificial intelligence to advance shark biodiversity monitoring. By substantially reducing manual annotation demands while maintaining high detection and classification performance, this framework offers a practical solution to speed up BRUV post-processing and filtering of digital observations. At the same time, it demonstrates automated methods as crucial tools for conservation and management, especially to address species-specific data deficiencies in shark research.

Large‐scale marine protected areas (LSMPAs; > 1000 km²) provide important refuge for large mobile species, but most do not encompass species' ranges. To better understand current and future LSMPA value, we concurrently tracked nine species (seabirds, cetaceans, pelagic fishes, manta rays, reef sharks) at Palmyra Atoll and Kingman Reef (PKMPA) in the U.S. Pacific Islands Heritage Marine National Monument. PKMPA and the U.S. Exclusive Economic Zone encompassed 39% and 54% of species movements (n = 83; tracking duration range: 0.5–350 days), respectively. Species distribution models indicated 73% of PKMPA contained highly suitable habitat. Under two projected future scenarios (SSP 1–2.6, “Sustainability”; SSP 3–7.0, “Rocky Road”), strong sea surface temperature gradients initially could cause abrupt oceanic change resulting in predicted habitat loss in 2040–2050, followed by an equilibrium response and regained habitat by 2090–2100. Current and future suitable habitats were available adjacent to PKMPA, suggesting that increased MPA size could enhance protection. Our three‐tiered approach combining animal tracking with publicly available remote sensing data and future projected environmental scenarios could be used to design, study, and monitor protected areas throughout the world. Holistic approaches that encompass diverse species and habitat use can enhance assessments of protected area designs. Animal telemetry and remote sensing may be helpful for ascertaining the extent to which other MPAs protect large mobile species in the future.

Tuna are known for exceptional swimming speeds, which are possible because of their thunniform lift-based propulsion, large muscle mass and rigid fusiform body. A rigid body should restrict maneuverability with regard to turn radius and turn rate. To test if turning maneuvers by the Pacific bluefin tuna (Thunnus orientalis) are constrained by rigidity, captive animals were videorecorded overhead as the animals routinely swam around a large circular tank or during feeding bouts. Turning performance was classified into three different types: (1) glide turns, where the tuna uses the caudal fin as a rudder; (2) powered turns, where the animal uses continuous near symmetrical strokes of the caudal fin through the turn; and (3) ratchet turns, where the overall global turn is completed by a series of small local turns by asymmetrical stokes of the caudal fin. Individual points of the rostrum, peduncle and tip of the caudal fin were tracked and analyzed. Frame-by-frame analysis showed that the ratchet turn had the fastest turn rate for all points with a maximum of 302 deg s−1. During the ratchet turn, the rostrum exhibited a minimum global 0.38 body length turn radius. The local turn radii were only 18.6% of the global ratchet turn. The minimum turn radii ranged from 0.4 to 1.7 body lengths. Compared with the performance of other swimmers, the increased flexion of the peduncle and tail and the mechanics of turning behaviors used by tuna overcomes any constraints to turning performance from the rigidity of the anterior body morphology.

Summary: The bluefin tuna heart remains at ambient water temperature (Ta) but must supply blood to warm regions of the body served by countercurrent vascular heat exchangers. Despite this unusual physiology, inherent difficulties have precluded an understanding of the cardiovascular responses to Ta in free-swimming bluefin tunas. We measured the heart rate (fH) responses of two captive Pacific bluefin tunas (Thunnus orientalis; 9.7 and 13.3 kg) over a cumulative period of 40 d. Routine fH during fasting in the holding tank at a Ta of 20°C was 45.1±8.0 and 40.7±6.5 beats min-1 for Tuna 1 and Tuna 2, respectively. fH decreased in each fish with Q10 of 2.6 (Tuna 1) and 3.1 (Tuna 2) as Ta in the tank was slowly decreased to 15°C (~0.4oC h-1), despite a gradual increase in swimming speed. The same thermal challenge during digestion revealed similar thermal dependence of fH and indicated that the rate of visceral cooling is not buffered by the heat increment of feeding. Acutely decreasing Ta from 20 to 10°C while Tuna 1 swam in a tunnel respirometer caused a progressive increase in tail beat-frequency and oxygen consumption rate (Mo2). fH of this fish decreased with Q10 of 2.7 as Ta decreased between 20 and 15°C, while further cooling to 10°C saw a general plateau in fH around 35 beats min-1 with Q10 of 1.3. A discussion of the relationships between fH, Mo2 , and haemoglobin-oxygen binding sheds further light on how bluefin cardiorespiratory systems function in a changing thermal environment.