Graziella Do Nascimento Silva

This study aimed to conduct an integrated analysis (systematic and exploratory bibliometric) on Cocos nucifera L. residues as a source of bioactive compounds, using the Scopus and Web of Science databases and VOSviewer and bibliometrix software. A total of 27 documents were identified between 2008 and 2024, reflecting research interest in this scope. The highest number of publications occurred in 2023 (5 articles), reflecting the strengthening of the circular bioeconomy and the valorization of residues, associated with the use of more sustainable extractive technologies and experimental optimization strategies. The term "Cocos nucifera" was central to the network, integrating studies on chemical characterization, bioactivities, and potential applications of residues from the species. The results presented in the Sankey Diagram reinforce the interdisciplinary nature of the topic and indicate connections between authors, institutions, and journals in different application fronts. However, as it is a reduced corpus, these patterns should be interpreted only as an internal organization of the analyzed literature. Residues have evolved from environmental liabilities to valuable bioactive matrices. Underutilized parts of Cocos nucifera L., such as husk, mesocarp, testa, haustorium, and roots, have been investigated as potential sources of phenolic compounds, flavonoids, and other metabolites of interest. The analyzed studies also indicate advances in the use of more sustainable extraction methods, such as ultrasound and supercritical CO₂, as well as modeling and optimization tools to improve process efficiency. The available evidence suggests antioxidant, antimicrobial, anti-inflammatory, antidiabetic, and hepatoprotective activities, although, for the most part, these are still associated with in vitro assays, experimental models, or laboratory applications. In this context, the extracts show potential for future applications in cosmeceuticals, functional foods, post-harvest conservation, and technological valorization of plant biomass, provided that additional studies on standardization, stability, safety, scaling, and validation in real systems are carried out.

The enzyme β-galactosidase is widely used in industrial processes, especially in the development of lactose-free or reduced products. Therefore, the objective of this study was to define the best fermentation conditions for the production of β-galactosidase from Aspergillus niger in cheese whey (CW) and to evaluate the purification of this enzyme through an Aqueous Two-Phase System (ATPS) composed of polyethylene glycol (PEG) and potassium phosphate. To verify the synergistic effects of different carbon and nitrogen sources, pH, and temperature on the production and optimization of the enzyme, a Factorial Design followed by a Rotational Central Composite Design (RCCD) was used. The highest activity was 50.30 U mL ^− 1 using the RCCD with CW (20% v/v) supplemented with lactose (13 g L ^− 1 ), yeast extract (7.5 g L ^− 1 ), and peptone (5 g L ^− 1 ), in a medium at 35 °C and pH 5.0. The composed of PEG 1500 g mol ^− 1 (20% w/w) and potassium phosphate (10% w/w) at pH 7.0 is the best condition to partially purify this enzyme, allowing a purification factor of 4.53, with a partition coefficient of 6.17 ± 0.40 and yield of 92.54%. These results demonstrate the possibility of using an ATPS composed of PEG-phosphate to purify a β-galactosidase from Aspergillus niger produced in CW supplemented with carbon and nitrogen sources. The study provides a low-cost and sustainable enzyme production strategy using CW and a simple and efficient purification with ATPS, which may be of great biotechnological interest.

The use of microalgae and natural coagulants in biological water treatment has been explored, as the use of chemical products can generate contaminants harmful to human health. Chlorella vulgaris primarily uses nitrogen and phosphorus as nutrients, managing to develop in various media where these and other compounds are present, removing them. In coagulation processes, Moringa oleifera is able to remediate effluents and raw water in a biodegradable manner, being an effective substitute for aluminum and iron-based coagulants and avoiding problems caused by them. Experiments with Chlorella vulgaris and Moringa oleifera were carried out in order to, firstly, analyze the growth of the microalga in the water of the Poxim River and verify its efficiency in removing chemical substances, followed by the evaluation of the capacity of Moringa oleifera in removing the microalga. The results showed that Chlorella vulgaris completely removed chlorite and glyphosate and reduced the concentration of other compounds, including nitrate, phosphorus, and iron. This study has proven that microalgae can be used to remove contaminants from raw water and that Moringa oleifera helps the microalgae to settle more quickly.

O soro de queijo é um subproduto correspondente à porção líquida resultante da produção de queijo e outros produtos lácteos, é rico em lactose, proteínas e micronutrientes. O descarte incorreto do soro de queijo é uma preocupação ambiental, por isso surge a necessidade de reaproveitamento desse resíduo. O soro de queijo vem sendo utilizado como substrato barato para a produção de enzimas industrialmente importantes, como a β-galactosidase, algumas linhagens de microalgas têm sido cultivadas em águas residuais para produção desta enzima, sendo a Chlorella vulgaris uma de grande destaque devido à sua alta capacidade de remoção de nutrientes (N e P) e demanda química de oxigênio (DQO). Desse modo, esse trabalho teve objetivo produzir β–galactosidase utilizando a microalga Chlorella vulgaris tendo o soro de queijo como substrato. O permeado de soro de queijo foi preparado e testado em várias concentrações completados com BG-11 (meio de cultivo para a microalga), depois disso foram realizados cultivos variando a concentração de uma das fontes de N do meio, durante 5 dias. A atividade enzimática máxima obtida foi de 8,88 U L-1 no cultivo contendo 60% (v/v) de permeado e meio BG-11 com 2300 mg L-1 de NaNO3, seguidos pelo cultivo de 20% (v/v) de permeado com 700 mg L-1 de NaNO3 e 20% (v/v) de permeado com 1500 mg L-1 de NaNO3, concentração de 6,29 U L-1 e 5,55 U L-1, respectivamente. Evidenciando que o permeado de soro de queijo foi um bom meio de cultivo para a produção da enzima β-galactosidase por Chlorella vulgaris.

Microalgae have a high growth rate, high CO₂ absorption capacity, and high content of chlorophyll, proteins, vitamins, mineral salts, carbohydrates, antioxidant substances, and fatty acids. In recent years, Chlorella vulgaris has been widely used as a feedstock for producing third-generation biofuels, such as bioethanol. Thus, this work aims to develop a strategy to increase the production scale of the microalgae Chlorella vulgaris grown in distilled reused water, supplemented with a modified BG-11 medium, to use biomass in the production of bioethanol. The total cultivation of 72 L presented a concentration of 0.415 g·L⁻¹·d⁻¹, with 61.32 g of final biomass. To improve carbohydrate extraction, the biomass was pre-treated with sulfuric acid at different concentrations (1.5% and 3% v/v). The hydrolyzed solution was supplemented with YPD (yeast extract peptone dextrose) medium and inoculated with Saccharomyces cerevisae yeast, initiating fermentation. In each sample, the Brix degree, cell concentration, reducing sugar concentration, and alcohol content were analyzed. The sample pre-treated with sulfuric acid 1.5% v/v was the one that presented the best result, with alcohol content after distillation of 68 °GL (Gay-Lussac). It appears that the cultivation of the microalgae Chlorella vulgaris in scale-up, with reused water, has high potential in the production of third-generation biofuel.

The worldwide production of microbial enzymes is generally limited by the cost of the substrate used for the cultivation of microorganisms. Thinking of a low-cost and abundant lignocellulosic substrate, coconut fiber is an alternative because it is rich in cellulose, hemicellulose, and lignin, presenting itself as a promising raw material for the production of an enzyme cocktail, which can be used in the production of second-generation biofuels. The objective of this work was to produce cellulolytic enzymes from microorganisms isolated from coconut fiber. In the production of the enzyme, the kinetic profile of the enzymatic complex was evaluated, analyzing the effects of moisture content, temperature, and pH during solid-state fermentation of coconut fiber, using a microorganism isolated from the bagasse itself. Statistica software was used to evaluate the enzyme complex production parameters. Statistical results indicated that the best humidity, temperature, and pH conditions were 80%, 30°C and 3, respectively, with activities of 119.93 U/g CMCase (endoglucanase) and 89.32 U/g FPase (total cellulases ) over a period of 48 h.