Xin Chen
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Xin Chen has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Ziyu Wang
Yazhou Bay Innovation Institute, Hainan Tropical Ocean University, Sanya 572025, China
3 shared publications - Peng Cao
College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China
2 shared publications - Wenjing Chen
Yazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, China
2 shared publications - Yu Xie
Yazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, China
2 shared publications - Liang Cao
College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China
1 shared publication - Rui Ding
College of Big Data Application and Economics (Guiyang College of Big Data Finance), Guizhou University of Finance and Economics, Guiyang, China
1 shared publication
Latest Publications
- Open Access
- 24 April 2025
- Published by MDPI AG in Journal of Marine Science and Engineering
- Vol. 13 (5) , 848
A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes
Microbially Induced Calcium Carbonate Precipitation (MICP) technology has garnered significant attention in geotechnical engineering and environmental remediation due to its environmentally friendly and cost-effective advantages. However, the current MICP technology faces challenges in practical engineering applications, particularly the prolonged cementation time, which makes it difficult to meet the requirements for coastal slope reinforcement. Therefore, this study designed novel cultivation conditions for Sporosarcina pasteurii by regulating external nitrogen source concentration and evaluated its environmental adaptability by measuring OD600, urease activity, and bacterial length. By monitoring the changes in Ca2+ concentration, pH, and precipitation rate over time during the mineralization process, rapid cementation under MICP conditions was achieved. The engineering applicability of this approach in slope reinforcement was comprehensively assessed through simulated on-site scouring and penetration tests. The reinforcement mechanism and the microstructure of the cementation under novel cultivation conditions were analyzed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). The results indicated that the activity of Sporosarcina pasteurii in the modified NH4-YE medium significantly improved in freshwater environments, and the MICP mineralization reaction was rapid, completing within 4 h. The primary crystal form of the generated precipitate was rhombohedral calcite, which formed a tightly bonded microstructure with calcareous sand, achieving a maximum reinforcement strength of 13.61 MPa. The penetration strength increased by at least 20%, and the precipitation rate improved by at least 2-fold. The scouring morphology remained essentially unchanged within 6 h. The findings of this study provide foundational and theoretical data for the application of MICP reinforcement technology to coastal calcareous sand models.
- 24 April 2025
- Published by MDPI AG in Journal of Marine Science and Engineering
- Vol. 13 (5) , 848
Open AccessA Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes
Microbially Induced Calcium Carbonate Precipitation (MICP) technology has garnered significant attention in geotechnical engineering and environmental remediation due to its environmentally friendly and cost-effective advantages. However, the current MICP technology faces challenges in practical engineering applications, particularly the prolonged cementation time, which makes it difficult to meet the requirements for coastal slope reinforcement. Therefore, this study designed novel cultivation conditions for Sporosarcina pasteurii by regulating external nitrogen source concentration and evaluated its environmental adaptability by measuring OD600, urease activity, and bacterial length. By monitoring the changes in Ca2+ concentration, pH, and precipitation rate over time during the mineralization process, rapid cementation under MICP conditions was achieved. The engineering applicability of this approach in slope reinforcement was comprehensively assessed through simulated on-site scouring and penetration tests. The reinforcement mechanism and the microstructure of the cementation under novel cultivation conditions were analyzed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). The results indicated that the activity of Sporosarcina pasteurii in the modified NH4-YE medium significantly improved in freshwater environments, and the MICP mineralization reaction was rapid, completing within 4 h. The primary crystal form of the generated precipitate was rhombohedral calcite, which formed a tightly bonded microstructure with calcareous sand, achieving a maximum reinforcement strength of 13.61 MPa. The penetration strength increased by at least 20%, and the precipitation rate improved by at least 2-fold. The scouring morphology remained essentially unchanged within 6 h. The findings of this study provide foundational and theoretical data for the application of MICP reinforcement technology to coastal calcareous sand models.
- Open Access
- 25 March 2024
- Published by MDPI AG in Journal of Marine Science and Engineering
- Vol. 12 (4) , 542
Research on the Effect of Natural Seawater in Domesticating Bacillus pasteurii and Reinforcing Calcareous Sand
Microbial-Induced Calcium Carbonate Precipitation (MICP) is an environmentally friendly, efficient, and sustainable new soil reinforcement technology. For this study, Bacillus pasteurii were domesticated and cultured in a natural seawater environment with multiple gradients and used for coral reef calcareous sand reinforcement, comparing the mineral composition of the generated precipitates and the reinforcement strength under different domestication gradient conditions. The results revealed that, while the natural seawater environment inhibits the growth of Bacillus pasteurii, the gradient domestication method allows the bacteria to gradually adapt to the natural seawater environment. Notably, their shape becomes thin and long under the seawater environment. Furthermore, the MICP mineralisation reaction rate is faster in the natural seawater environment and, with an increase in the domestication gradient, the mineralisation reaction precipitates increased. At the same time, in the seawater environment, a small amount of mineral components were generated in addition to , such as , and the mineral content increased with an increase in the domestication gradient. When comparing the curing effect under different gradients in the natural seawater environment, it was found that the Bacillus pasteurii can effectively enhance the curing effect of the calcareous sand after multi-gradient domestication in the seawater environment, with the curing effect increasing with an increase in the domestication gradient. The results of this study provide new ideas for the application of MICP technology in seawater environments for the reinforcement of calcareous sand in the construction of South China Sea islands and reefs.- 25 March 2024
- Published by MDPI AG in Journal of Marine Science and Engineering
- Vol. 12 (4) , 542
Open AccessResearch on the Effect of Natural Seawater in Domesticating Bacillus pasteurii and Reinforcing Calcareous Sand
Microbial-Induced Calcium Carbonate Precipitation (MICP) is an environmentally friendly, efficient, and sustainable new soil reinforcement technology. For this study, Bacillus pasteurii were domesticated and cultured in a natural seawater environment with multiple gradients and used for coral reef calcareous sand reinforcement, comparing the mineral composition of the generated precipitates and the reinforcement strength under different domestication gradient conditions. The results revealed that, while the natural seawater environment inhibits the growth of Bacillus pasteurii, the gradient domestication method allows the bacteria to gradually adapt to the natural seawater environment. Notably, their shape becomes thin and long under the seawater environment. Furthermore, the MICP mineralisation reaction rate is faster in the natural seawater environment and, with an increase in the domestication gradient, the mineralisation reaction precipitates increased. At the same time, in the seawater environment, a small amount of mineral components were generated in addition to , such as , and the mineral content increased with an increase in the domestication gradient. When comparing the curing effect under different gradients in the natural seawater environment, it was found that the Bacillus pasteurii can effectively enhance the curing effect of the calcareous sand after multi-gradient domestication in the seawater environment, with the curing effect increasing with an increase in the domestication gradient. The results of this study provide new ideas for the application of MICP technology in seawater environments for the reinforcement of calcareous sand in the construction of South China Sea islands and reefs. - Open Access
Experimental Study on Coastal Sediment Reinforcement by Induced Carbonate Precipitation by Different Enzyme Sources
Coastal erosion is increasing worldwide due to the increasing frequency of extreme natural phenomena and excessive human exploitation. In this study, a small model experiment was conducted to investigate the solidification effects of three enzyme sources—soybean urease, freshwater Bacillus pasteurella, and seawater domesticated Bacillus pasteurella—on coastal sediments and their impacts in a seawater environment. The solidifying effect of different enzyme sources was determined by measuring the mechanical properties and corrosion resistance of the cured specimen model. The influence of solidified seawater in a seawater environment was obtained by measuring the changes in the pH value, calcium ion concentration, and ammonia nitrogen content of solidified seawater. The results show that different enzyme sources have a certain strengthening effect on coastal sediments. The mechanical properties of coastal sediments can be enhanced by increasing the amount of enzyme solution or level of solidification and can effectively resist simulated flow erosion. Comparing the reinforcement effects of different enzyme sources, it can be seen. It was observed that Bacillus pasteurella acclimated in seawater had better reinforcement effects than Bacillus pasteurella fresh water, and Bacillus pasteurella fresh water had better reinforcement effects than soybean urease. In the seawater measurement tests, the solidification of coastal sediments using different enzyme sources led to a decrease in the seawater pH value, and the acidification of seawater dissolved the generated calcium carbonate, increased the concentration of calcium ions in seawater, and produced ammonia nitrogen as a byproduct in the seawater. It was observed that, compared with the other two enzyme source solutions, the seawater-domesticated Bacillus pasteurella can better adapt to the high-salt environment of seawater, microbial metabolism is not inhibited, urea decomposition ability is improved, and calcium carbonate production is higher, which can effectively improve the engineering characteristics of coastal sediments and play a positive role in coastal protection and development.
Open AccessExperimental Study on Coastal Sediment Reinforcement by Induced Carbonate Precipitation by Different Enzyme Sources
Coastal erosion is increasing worldwide due to the increasing frequency of extreme natural phenomena and excessive human exploitation. In this study, a small model experiment was conducted to investigate the solidification effects of three enzyme sources—soybean urease, freshwater Bacillus pasteurella, and seawater domesticated Bacillus pasteurella—on coastal sediments and their impacts in a seawater environment. The solidifying effect of different enzyme sources was determined by measuring the mechanical properties and corrosion resistance of the cured specimen model. The influence of solidified seawater in a seawater environment was obtained by measuring the changes in the pH value, calcium ion concentration, and ammonia nitrogen content of solidified seawater. The results show that different enzyme sources have a certain strengthening effect on coastal sediments. The mechanical properties of coastal sediments can be enhanced by increasing the amount of enzyme solution or level of solidification and can effectively resist simulated flow erosion. Comparing the reinforcement effects of different enzyme sources, it can be seen. It was observed that Bacillus pasteurella acclimated in seawater had better reinforcement effects than Bacillus pasteurella fresh water, and Bacillus pasteurella fresh water had better reinforcement effects than soybean urease. In the seawater measurement tests, the solidification of coastal sediments using different enzyme sources led to a decrease in the seawater pH value, and the acidification of seawater dissolved the generated calcium carbonate, increased the concentration of calcium ions in seawater, and produced ammonia nitrogen as a byproduct in the seawater. It was observed that, compared with the other two enzyme source solutions, the seawater-domesticated Bacillus pasteurella can better adapt to the high-salt environment of seawater, microbial metabolism is not inhibited, urea decomposition ability is improved, and calcium carbonate production is higher, which can effectively improve the engineering characteristics of coastal sediments and play a positive role in coastal protection and development.