Gabrielly Diniz Duarte

A problemática das intoxicações exógenas causa impacto significativo na saúde da população e no contexto das drogas de abuso essas complicações podem causar sérios danos, pois são produtos de fácil acesso e frequentemente utilizados sem supervisão profissional. Este estudo focou em definir e analisar o perfil epidemiológico e clínico das intoxicações exógenas por drogas de abuso notificadas na região Nordeste, com base no Sistema de Informação de Agravos de Notificação – SINAN, a partir do portal DATASUS. Foi um estudo descritivo com abordagem transversal, retrospectiva e quantitativa. A população do estudo incluiu todos os casos de intoxicações exógenas por drogas de abuso na região Nordeste entre 2017 e 2022, coletando dados sobre ano de notificação, sexo, idade e circunstância da intoxicação. Entre 2017 e 2022, foram registrados 17.905 casos de intoxicação por drogas de abuso no Nordeste do Brasil, com maior prevalência em Pernambuco (46,24%) e no Rio Grande do Norte (14,50%). Em 2019, houve um aumento de 19,67% nas notificações. A categoria "ignorados e brancos" predominou em todas as variáveis, exceto sexo, com 75,40% dos casos ocorrendo em homens. A forma de abuso foi a circunstância mais comum, resultando principalmente em cura sem sequelas. A confirmação clínica foi predominante, destacando a necessidade de melhorar a prevenção e o controle para proteger usuários e profissionais.

In 2019, the emergence of the seventh known coronavirus to cause severe illness in humans triggered a global effort towards the development of new drugs and vaccines for the SARS-CoV-2 virus. These efforts are still ongoing in 2024, including the present work where we conducted a ligand-based virtual screening of terpenes with potential anti-SARS-CoV-2 activity. We constructed a Quantitative Structure–Activity Relationship (QSAR) model from compounds with known activity against SARS-CoV-2 with a model accuracy of 0.71. We utilized this model to predict the activity of a series of 217 terpenes isolated from the Fabaceae family. Four compounds, predominantly triterpenoids from the lupane series, were subjected to an in vitro phenotypic screening in Vero CCL-81 cells to assess their inhibitory activity against SARS-CoV-2. The compounds which showed high rates of SARS-CoV-2 inhibition along with substantial cell viability underwent molecular docking at the SARS-CoV-2 main protease, papain-like protease, spike protein and RNA-dependent RNA polymerase. Overall, virtual screening through our QSAR model successfully identified compounds with the highest probability of activity, as validated using the in vitro study. This confirms the potential of the identified triterpenoids as promising candidates for anti-SARS-CoV-2 therapeutics.

2019-n-CoV is the seventh coronavirus known to infect humans and cause serious illness, and due to its lethality there was a worldwide mobilization for the development of new drugs and new vaccines for Covid-19. Therefore, the present work aims to carry out a virtual screening of terpenes with potential anti SARS-CoV-2 activity. To perform the ligand-based virtual, a model was constructed regarding the compounds which already showed inhibitory activity of the SARS-CoV-2. The test series corresponded to terpenes isolated in the Fabaceae Family. The compounds that were most likely to activity were subjected to Molecular Docking and the best poses obtained were subjected to Molecular Dynamics (MD) simulations carried. The molecules under study were submitted to in vitro evaluation to assess inhibitory activity of compounds against SARS-CoV-2 in a phenotypic screening model of the virus. The compounds selected by in silico screening corresponded to triterpenoids from the lupane series. In the in vitro test, both demonstrated activity against the SARS-CoV-2 virus. The virtual screening performed managed to identify the compounds with the highest probability of activity, as well as the most stable compounds, and the method used was validated by the in vitro study carried out.

Leishmaniasis is a group of neglected infectious, non-contagious diseases caused by parasites of the Leishmania genus which affects millions of people worldwide. The drugs available for its treatment, especially in cases of visceral leishmaniasis, are old, outdated, have serious side effects and in many cases are ineffective due to the emergence of resistant strains. In this context, the search for new candidates for more effective and safer drugs which can become new therapeutic alternatives is a constant need. In this work, 10 new chalcones were synthesized in high yields through Claisen-Schmidt condensation and were evaluated in vitro against Leishmania infantum promastigotes and amastigotes. CP04 and CP06 compounds were the most promising, respectively showing IC50 values = 13.64 ± 0.25 and 11.19 ± 0.22 against promastigotes, and IC50 = 18.92 ± 0.05 and 22.42 ± 0.05 against amastigotes. The compounds did not show cytotoxicity in erythrocytes, showing selectivity indexes greater than 74 and 90. Molecular docking studies carried out under the sterol 14-alpha demethylase (CYP-51) (PDB: 3L4D) and trypanothione reductase (PDB: 5EBK) enzymes from L. infantum evidenced the great affinity of the CP04 molecule for these targets, showing Moldock score values of -94.0758 and -50.5692 KJ/mol-1, with these values being much lower than the co-crystallized ligand energies. Molecular dynamics simulations demonstrated great stability of CP04 in its targets, thus indicating a real interaction possibility. Our findings confirm the potential that chalcones have as candidates for anti-leishmania drugs, and suggest that the CP04 compound may become a promising drug candidate to design and develop novel chalcone-based analogs for leishmaniosis therapy.

Chalcones are direct precursors in the biosynthesis of flavonoids. They have an α,β-unsaturated carbonyl system which gives them broad biological properties. Among the biological properties exerted by chalcones, their ability to suppress tumors stands out, in addition to their low toxicity. In this perspective, the present work explores the role of natural and synthetic chalcones and their anticancer activity in vitro reported in the last four years from 2019 to 2023. Moreover, we carried out a partial least square (PLS) analysis of the biologic data reported for colon adenocarcinoma lineage HCT-116. Information was obtained from the Web of Science database. Our in silico analysis identified that the presence of polar radicals such as hydroxyl and methoxyl contributed to the anticancer activity of chalcones derivatives. We hope that the data presented in this work will help researchers to develop effective drugs to inhibit colon adenocarcinoma in future works.

Este artigo trata da anatomia macroscópica veterinária como ferramenta educacional no ensino fundamental e médio no Brasil e a forma como é ensinada nas escolas públicas brasileiras. O objetivo principal é conhecer o desenvolvimento do ensino macroscópico na educação básica, a fim de demonstrar a relevância de se conhecer a anatomia dos animais na prática. Sabe-se que o ensino de anatomia humana e animal está vinculado ao ensino das ciências biológicas, porém, percebemos que o ensino / aprendizagem desses alunos tem sido insuficiente, pois apresentam aulas práticas associadas ao desinteresse de. anatomia macroscópica, havendo também uma certa indiferença por parte dos professores e da própria instituição em fornecer recursos que levem os professores a trazer o conhecimento da teoria para a prática. Este estudo é evidenciado pelo caráter qualitativo, retirando do objetivo da pesquisa a resposta à questão problematizadora acima.

Síntese e avaliação antitumoral do (±)-4-O-demethylKadsurenin M um composto natural e seus análogos/ Synthesis and tumoral test of (±)-4-O-DemethylKadsurenin M a natural compound and analogues