Mustafa Kemal Basarali

CRISPR-based epigenetic editing enables reversible regulation of gene expression without permanent DNA modification. The integration of artificial intelligence (AI) enhances guide RNA (gRNA) design, off-target prediction, and delivery optimization. We conducted a systematic review and meta-analysis (2015–2025) in accordance with PRISMA 2020 guidelines to evaluate the impact of AI on the precision, safety, and therapeutic efficacy of epigenetic CRISPR tools. From 540 screened records, 58 studies met inclusion criteria, of which 41 provided extractable quantitative data for meta-analysis and 17 contributed to qualitative synthesis. Random-effects models, subgroup analyses, and bias assessments were applied. Pooled analyses demonstrated strong positive effects across three domains: therapeutic efficacy (SMD = 1.67), gRNA optimization (SMD = 1.44), and off-target prediction (AUC = 0.79). Publication bias was minimal, and subgroup analyses indicated the strongest impact in therapeutic applications. Deep learning models were consistently associated with higher effect sizes. Qualitative synthesis revealed trends in interpretable AI, omics integration, and delivery innovations, underscoring AI’s role in safer and more precise CRISPR editing. Overall, AI significantly improves the precision and therapeutic performance of CRISPR-based epigenetic tools, with the strongest effects observed in therapeutic efficacy, supporting their potential for personalized gene editing.

Soluble triggering receptor expressed on myeloid cells (sTREM-1) is a new biomarker that can be used for the diagnosis and monitoring of urinary system infections. This study aimed to evaluate the diagnostic performance of serum sTREM-1 in patients with a diagnosis of acute stone pyelonephritis (ASP). This prospective study included 46 patients with a diagnosis of ASP and a control group of 23 individuals without urinary system infection. Blood samples were taken from participants upon hospital admission, and basal serum sTREM-1 levels were analyzed using the ELISA method. Serum sTREM-1 concentrations were measured after treatment of ASP patients. Basal leukocyte counts, C-reactive protein (CRP) levels, procalcitonin (PCT), and sTREM-1 (98.6 vs. 68.4 pg/mL, p < 0.001) levels were higher in the ASP group compared to the control group. After treatment, the median leukocyte counts, PCT, and sTREM-1 levels decreased and were similar to those of the control group. The median CRP level also decreased after treatment, but it remained higher than that of the control group. In predicting patients with ASP, the baseline sTREM-1 exhibited a sensitivity of 74.6% and a specificity of 78.2%, while its diagnostic performance was lower than that of leukocyte counts, CRP, and PCT. Despite the findings that levels of sTREM-1 were higher upon hospital admission in patients with ASP and significantly decreased after treatment, the utility of sTREM-1 as a biomarker for predicting patients with ASP remains constrained when compared to established inflammatory markers.

Amaç: Çalışmanın amacı 112 Acil Ambulans hizmetlerinde (hastane öncesi acil sağlık hizmetleri) görev yapan çalışanların iş performansını etkileyen faktörlerin belirlenmesidir. Yöntem: Kesitsel tipte planlanan bu çalışmanın verileri, 01/01/2016–31/03/2016 tarihleri arasında Mersin ilinde toplandı. Çalışmaya 378 ambulans (112 Acil Servis) çalışanı katılmıştır. Çalışmada, iş performans ölçeği, örgütsel bağlılık ölçeği ve duygusal tükenmişlik ölçeği kullanılmıştır. Verilerin değerlendirilmesinde güvenirlik testi, yüzdelik dağılım, ortalama, standart sapma, korelasyon ve regresyon analizleri kullanılmıştır. Bulgular: Katılımcıların yaş ortalaması 28.9±6.16, toplam çalışma yılı 6.0±3.273, %68.3’ü kadın, %63.0’ı evli, %91.0’ı Acil ve Ambulans Bakım Teknikeri/ Acil Tıp Teknisyeni, %43.9’u önlisans mezunudur. İş performans skoru 4.1, örgütsel bağlılık skoru 3.5 iken duygusal tükenmişlik skoru 2.6 çıkmıştır. Korelasyon analizine göre iş performansı ile örgütsel bağlılık arasında pozitif, iş performansı ile duygusal tükenmişlik arasında ise negatif ilişki bulunmuştur. Regresyon analizine göre ise iş performansını sadece örgütsel bağlılığın pozitif yönde etkilediği belirlenmiştir. Sonuç: Ambulans çalışanları ile yapılan bu çalışmada, iş performansı ile duygusal tükenmişlik ve örgütsel bağlılık düzeyleri arasında anlamlı ilişkiler bulunmuştur. Bu açıdan çalışanların, performans düzeylerinin yükseltilmesi için örgüte bağlılık düzeylerinin artırılması ve tükenmişlik seviyelerinin azaltılmasının önemli olacağı düşünülmektedir. Dönem dönem çalışanların bağlılık ve tükenmişlik seviyelerinin ölçülmesi gerekmektedir. Ölçüm sonucuna yönelik olarak müdahale programlarının geliştirilmesinin iş performansı açısından önemli olduğu düşünülmektedir.

In order to understand whether exercise and caffeic acid phenethyl ester (CAPE) has an effect on obesity and weight control, we investigated the effects of CAPE, and exercise on lipid parameters (triglyceride, total cholesterol, HDL-C, LDLC), and adipokine substances such as leptin and resistin in rats. 40 male rat were randomly assigned into 4 groups. It was determined that CAPE does not have any significant effect on these parameters but that lipid parameters and leptin values in exercise groups decreased considerably, while no significant change occurred in resistin levels. In order to understand whether diet has an effect on exercise, body weights of all animal groups in pre and post-exercise were compared. A significant weight gain was observed (p = 0.005) in all groups. This study concluded that exercise has a considerable effect on leptin and lipid parameters; however, exercise alone was not sufficient for weight control and could be effective in weight control only when accompanied by a restricted diet.