A. Pagnotta
Research Subjects
Research Interests
A. Pagnotta has a total of 2 co-authors. Here are the top co-authors by number of shared publications:
F. Greco
Institute of Orthopedics, University of Ancona, Largo Cappelli 1, 60100 Ancona, Italy
1 shared publication- Nicola Specchia
Department of Orthopedic Surgery, Umberto I General Hospital, Università Politecnica delle Marche, Ancona, Italy
1 shared publication
Latest Publications
- 1 January 2002
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 20 (1) , 163-168
Androgen receptors in Dupuytren's contracture
Palmar fascia tissue and cultured cells from patients with Dupuytren's contracture and from normal subjects were characterized and analyzed for androgen receptor expression. Androgen receptors have never been studied in Dupuytren's myofibroblasts and may have a role in its high male predominance. Surgical samples were collected from eight patients undergoing surgery for Dupuytren's contracture and from four patients with carpal tunnel syndrome, used as control tissue. Immunohistochemical analysis was performed on tissue samples and on cell cultures with anti‐androgen receptor, anti‐α‐smooth muscle actin, anti‐fibronectin, and anti‐type I and III collagen antibodies using the biotin–avidin peroxidase method as revelatory system. Immunostaining for androgen receptors in tissue samples and cultured cells revealed nuclear reaction in many Dupuytren's myofibroblasts, but in few fibroblasts of the normal palmar fascia. In a double‐labeling study, androgen receptors were seen to co‐localize with α‐actin in both cell cultures and tissue samples.We present the first evidence that the palmar fascia is a target tissue for androgen action and that the expression of androgen receptors in Dupuytren's contracture is considerably higher than in the normal palmar fascia. Further studies will need to evaluate whether the androgen‐responsive state of the tissue is related to the high incidence of Dupuytren's contracture in the male sex. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
- 1 January 2002
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 20 (1) , 163-168
Androgen receptors in Dupuytren's contracture
Palmar fascia tissue and cultured cells from patients with Dupuytren's contracture and from normal subjects were characterized and analyzed for androgen receptor expression. Androgen receptors have never been studied in Dupuytren's myofibroblasts and may have a role in its high male predominance. Surgical samples were collected from eight patients undergoing surgery for Dupuytren's contracture and from four patients with carpal tunnel syndrome, used as control tissue. Immunohistochemical analysis was performed on tissue samples and on cell cultures with anti‐androgen receptor, anti‐α‐smooth muscle actin, anti‐fibronectin, and anti‐type I and III collagen antibodies using the biotin–avidin peroxidase method as revelatory system. Immunostaining for androgen receptors in tissue samples and cultured cells revealed nuclear reaction in many Dupuytren's myofibroblasts, but in few fibroblasts of the normal palmar fascia. In a double‐labeling study, androgen receptors were seen to co‐localize with α‐actin in both cell cultures and tissue samples.We present the first evidence that the palmar fascia is a target tissue for androgen action and that the expression of androgen receptors in Dupuytren's contracture is considerably higher than in the normal palmar fascia. Further studies will need to evaluate whether the androgen‐responsive state of the tissue is related to the high incidence of Dupuytren's contracture in the male sex. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.