Vladislav Borisov
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Vladislav Borisov has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
- Anna Marsano
Department of Surgery and Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland
1 shared publication - Friedrich Stefan Eckstein
Department of Cardiac Surgery, University Hospital Basel , Basel, Switzerland
1 shared publication - Giulia Milan
Department of Surgery and Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland
1 shared publication - Giuseppe Isu
Laboratory of Cardiac Surgery and Engineering, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland
1 shared publication - Gregor Hutter
Department of Biomedicine, Basel University Hospital, Basel, Switzerland
1 shared publication - Gregory Reid
Department of Biomedical Engineering, University of Basel, 4123 Basel, Switzerland
1 shared publication
Latest Publications
- Open Access
- 4 July 2023
- Published by MDPI AG in Bioengineering
- Vol. 10 (7) , 800
Upscaled Skeletal Muscle Engineered Tissue with In Vivo Vascularization and Innervation Potential
Engineering functional tissues of clinically relevant size (in mm-scale) in vitro is still a challenge in tissue engineering due to low oxygen diffusion and lack of vascularization. To address these limitations, a perfusion bioreactor was used to generate contractile engineered muscles of a 3 mm-thickness and a 8 mm-diameter. This study aimed to upscale the process to 50 mm in diameter by combining murine skeletal myoblasts (SkMbs) with human adipose-derived stromal vascular fraction (SVF) cells, providing high neuro-vascular potential in vivo. SkMbs were cultured on a type-I-collagen scaffold with (co-culture) or without (monoculture) SVF. Large-scale muscle-like tissue showed an increase in the maturation index over time (49.18 ± 1.63% and 76.63 ± 1.22%, at 9 and 11 days, respectively) and a similar force of contraction in mono- (43.4 ± 2.28 µN) or co-cultured (47.6 ± 4.7 µN) tissues. Four weeks after implantation in subcutaneous pockets of nude rats, the vessel length density within the constructs was significantly higher in SVF co-cultured tissues (5.03 ± 0.29 mm/mm2) compared to monocultured tissues (3.68 ± 0.32 mm/mm2) (p < 0.005). Although no mature neuromuscular junctions were present, nerve-like structures were predominantly observed in the engineered tissues co-cultured with SVF cells. This study demonstrates that SVF cells can support both in vivo vascularization and innervation of contractile muscle-like tissues, making significant progress towards clinical translation.
- 4 July 2023
- Published by MDPI AG in Bioengineering
- Vol. 10 (7) , 800
Open AccessUpscaled Skeletal Muscle Engineered Tissue with In Vivo Vascularization and Innervation Potential
Engineering functional tissues of clinically relevant size (in mm-scale) in vitro is still a challenge in tissue engineering due to low oxygen diffusion and lack of vascularization. To address these limitations, a perfusion bioreactor was used to generate contractile engineered muscles of a 3 mm-thickness and a 8 mm-diameter. This study aimed to upscale the process to 50 mm in diameter by combining murine skeletal myoblasts (SkMbs) with human adipose-derived stromal vascular fraction (SVF) cells, providing high neuro-vascular potential in vivo. SkMbs were cultured on a type-I-collagen scaffold with (co-culture) or without (monoculture) SVF. Large-scale muscle-like tissue showed an increase in the maturation index over time (49.18 ± 1.63% and 76.63 ± 1.22%, at 9 and 11 days, respectively) and a similar force of contraction in mono- (43.4 ± 2.28 µN) or co-cultured (47.6 ± 4.7 µN) tissues. Four weeks after implantation in subcutaneous pockets of nude rats, the vessel length density within the constructs was significantly higher in SVF co-cultured tissues (5.03 ± 0.29 mm/mm2) compared to monocultured tissues (3.68 ± 0.32 mm/mm2) (p < 0.005). Although no mature neuromuscular junctions were present, nerve-like structures were predominantly observed in the engineered tissues co-cultured with SVF cells. This study demonstrates that SVF cells can support both in vivo vascularization and innervation of contractile muscle-like tissues, making significant progress towards clinical translation.