Roshanak Abbasi

Recent advancements in material science have leveraged artificial intelligence (AI) to significantly enhance the design and synthesis of aluminum nanoparticles (Al NPs), enabling sub-nanometer control over structural and functional properties. By integrating machine learning (ML) algorithms with computational modeling, researchers have optimized nanoparticle size, morphology, and surface characteristics to support applications such as energy storage, catalytic reactions, and lightweight composites. AI-driven methods such as active learning and generative design streamline the discovery of novel synthesis routes, reducing trial-and-error and enabling scalable production. These nanoparticles (NPs) demonstrate a 15-30% increase in thermal stability, up to 2x enhancement in surface reactivity, and improved energy density compared to conventional counterparts, making them essential for sustainable technologies, aerospace engineering, and next-generation batteries. This innovation underscores the synergy between AI and nanotechnology, enabling rapid, cost-effective development of advanced materials with tailored functionalities.

Background The study was designed to explore the enhanced impact of nano-hydroxyapatite and emdogain on the survival and osteogenic/odontogenic differentiation of human stem cells isolated from the apical papilla (hSCAPs). Materials and Methods In this in vitro trial, hSCAPS obtained from intact impacted immature third molars were confirmed to have characteristic cell surface markers, then exposed to nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain for durations of 1–3 days. The survival of apical papilla stem cells was measured using a methyl thiazolyl tetrazolium assay. The quantitative reverse transcription polymerase chain reaction, alkaline phosphatase activity (ALP) and Alizarin red staining were used to evaluate osteogenic-odontogenic differentiation. Analysis of the data was done using one-way ANOVA, t-test, and Mann–Whitney test (α = 0.05). Results At 1–3 days, emdogain exhibited no significant impact on the survival of human stem cells from the apical papilla. In contrast, nanohydroxyapatite (α > 0.05) and nanohydroxyapatite coated with emdogain demonstrated a notable reduction in cell survival compared to the control group (α < 0.05). The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes demonstrated a notable increase in the group treated with nanohydroxyapatite coated with emdogain compared to the other groups (α < 0.05), and furthermore, this group exhibited more pronounced mineralized nodules than the other experimental groups. Conclusion In contrast to nanohydroxyapatite, Emdogain did not demonstrate a detrimental effect on the survival of hSCAPs. Nanohydroxyapatite, emdogain, and nanohydroxyapatite coated with emdogain increased osteogenic/odontogenic differentiation of hSCAPs.

Aim: This study compared the apical transportation and centering ratio of ProTaper Next (PTN) and XP‐endo Shaper (XPS) nickel titanium (NiTi) rotary files in curved root canals using cone beam computed tomography (CBCT). Methodology: The current in vitro study involved the mesiobuccal canals of mesial roots in 44 extracted mandibular first molars that exhibited apical curvature ranging from 10° to 30°. Two experimental groups were randomly formed from the teeth ( n = 22) and subjected to instrumentation with PTN and XPS. CBCT scans were performed before and after instrumentation on the teeth, and the apical transport and centering ratio were calculated at 3, 4, and 5 mm from the apex. Group comparisons were conducted using an independent t ‐test with a significance level set at alpha = 0.05. Results: Comparisons within groups did not reveal any statistically significant differences in the magnitude of canal transportation in the buccolingual (BL) and mesiodistal (MD) directions at any level from the apex, neither in the XPS group nor in the PTN group ( p > 0.05). Canal transportation in both BL and MD directions was significantly greater in PTN than in XPS ( p < 0.05). The centering ratio in the MD and BL directions was the same at 3, 4, and 5 mm from the apex in the PTN ( p > 0.05) and also in the XPS ( p > 0.05) groups. The centering ratio was significantly higher in XPS than in PTN ( p < 0.05) except at 5 mm from the apex in the MD direction and 4 and 5 mm from the apex in the BL direction ( p > 0.05). Conclusions: Both buccolingually and mesiodistally, PTN led to greater apical transport than XPS and also showed a lower centering ratio.

Background: The effectiveness of final root canal irrigants is crucial for successful conventional and regenerative endodontic treatments. This study aimed to systematically review the impact of final irrigants on dentin, disinfection, and regenerative potential in endodontic therapies. Methods: MEDLINE, Cochrane Library, and Web of Science databases were electronically searched for articles on final irrigants in conventional and regenerative endodontics, evaluating effects on dentin, smear layer, fracture resistance, stem cells, growth factors, and antimicrobial properties. The risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation risk of bias tool. Results: Overall, 25 eligible studies were included in this review after screening 2842 articles. Commonly assessed irrigants included ethylenediaminetetraacetic acid (EDTA) (21 studies), sodium hypochlorite (NaOCl; 12 studies), and citric acid (6 studies). EDTA could enhance growth factors but could weaken dentin. NaOCl affected dentin despite its antimicrobial benefits. Nanoparticles, chitosan, peracetic acid (PAA), and citric acid showed potential benefits. A meta-analysis was not performed due to methodological heterogeneity. Conclusion: In general, optimized irrigation strategies balancing disinfection, biocompatibility, and regenerative potential are necessary. More research is required to develop improved irrigation protocols. Registration: PROSPERO CRD42023420406.

Background: This investigation set out to compare the impacts of low-level diode laser (LLDL) and red light-emitting diode (LED) on the survival of human dental pulp stem cells (hDPSCs) and osteoge...

Purpose Currently, regenerative endodontic treatments are gaining more and more attention, and stem cells play a significant role in these treatments. In order to enhance stem cell proliferation and differentiation, a variety of methods and materials have been used. The purpose of this study was to determine the effects of magnesium oxide nanoparticles and LED irradiation on the survival and differentiation of human stem cells from apical papilla. Methods The MTT test was used to measure the cell survival of SCAPs that had been exposed to different concentrations of magnesium oxide nanoparticles after 24 and 48 h, and the concentration with the highest cell survival rate was picked for further studies. The cells were classified into four distinct groups based on their treatment: (1) control, which received no exposure, (2) exposure to magnesium oxide nanoparticles, (3) exposure to light emitting diode (LED) irradiation (635 nm, 200 mW/cm²) for 30 s, (4) exposure simultaneously with magnesium oxide nanoparticles and LED irradiation. A green approach was employed to synthesize magnesium oxide nanoparticles. Quantitative real time PCR was used to measure the gene expression of osteo/odontogenic markers such as BSP, DSPP, ALP and DMP1 in all four groups after treatment, and Alizarin red S staining (ARS) was used to determine the osteogenic differentiation of SCAPs by demonstrating the Matrix mineralization. Results The highest viability of SCAPs was observed after 24 h in concentration 1 and 10 µg/mL and after 48 h in concentration 1 µg/mL, which were not significantly different from the control group. In both times, the survival of SCAPs decreased with increasing concentration of magnesium oxide nanoparticles (MgONPs). According to the results of Real-time PCR, after 24 and 48 h, the highest differentiation of BSP, DMP1, ALP and DSPP genes was observed in the LED + MgONPs group, followed by MgONPs and then LED, and in all 3 experimental groups, it was significantly higher than control group (P < 0.05). Also, after 24 and 48 h, the density of ARS increased in all groups compared to the control group, and the highest density was observed in the MgONPs + LED and MgONPs groups. Conclusion This research concluded that exposure to SCAPs, MgONPs, and LED irradiation has a significant effect on enhancing gene expression of odontogenic/osteogenic markers and increasing matrix mineralization.

Introduction: Considering the positive impact of laser treatment on the proliferation of certain cell types, we opted to perform a systematic review aimed at evaluating the effects of laser therapy and photobiomodulation on the proliferation and differentiation of human dental pulp stem cells (hDPSCs). Methods: We included all research studies examining the impact of laser therapy on hDPSCs, without limitations on publication dates or article languages. The major international databases, including PubMed, ISI Web of Science, and Scopus, were searched from inception to April 2022 by the relevant keywords. Results: In total, 1886 studies were identified in the initial search from the mentioned databases and other sources. Finally, 17 relevant studies were included in the present systematic review after removing duplicates and non-relevant articles. The results indicated the useful effect of low-level laser therapy (LLLT) on the hDPSCs. Conclusion: The findings of this systematic review indicate the useful role of LLLT in cell therapy, proliferation, and differentiation associated with hDPSCs.

Background An experimental study was conducted to examine whether melatonin influences osteogenic/odontogenic differentiation of human stem cells derived from the apical papilla (hSCAPs). Materials and methods In order to isolate hSCAPs, the undeveloped root of a third molar of a human tooth was used. Melatonin was administered to the experimental groups in an osteogenic medium. No treatment was administered to the control group. The methyl thiazolyl tetrazolium (MTT) assay was performed on days 1, 2, and 3 to assess cell viability (n = 8). A determination of odontogenic/osteogenic differentiation was accomplished using alkaline phosphatase (ALP) activity alizarin red staining (ARS) (n = 6), and the expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR) (n = 3) on days 1, 2, and 7. Evaluation of the data was conducted using SPSS version 18. All experiments were conducted at least three times. The Mann Whitney U test, the ANOVA analysis, Tukey’s test, and t-test was implemented to analyze the data (α = 0.05). Results After 24 h, 48 h, and 72 h, No significant difference was observed between the control group and the melatonin treatment group in terms of viability of hSCAPs. (from 1 up to 10 µg/ml) (P > 0.05). The assessment of ARS and ALP activity showed that melatonin treatment enhanced osteogenic differentiation of hSCAPs (P < 0.001). Melatonin treatment caused hSCAPs to show an increase of genes related to osteogenic/odontogenic differentiation. These genes included ALP, dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) (P < 0.001). Conclusions Melatonin treatment enhanced osteogenic/odontogenic differentiation of hSCAPs with a dose dependent effect on cell viability.

Background: This experimental study aimed to assess the effect of irradiation of red light-emitting diode (LED) and Diode low-level laser (LLL) on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs). Materials and methods: SCAPs were isolated from the human tooth root. The experimental groups were subjected to 4 J/cm² diode low level laser and red LED irradiation in osteogenic medium. The control group did not receive any irradiation. Cell viability/proliferation of SCAPs was assessed by the methyl thiazolyl tetrazolium (MTT) assay on days 1 and 2 (n = 9). Osteogenic differentiation was evaluated by alizarin red staining (ARS) (n = 3), and expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR) (n = 12) on days 1 and 2. SPSS version 18 was used for data evaluation. The Kruskal-Wallis and Mann-Whitney tests were used to compare the groups at each time point. Results: The MTT assay showed no significant difference in cell viability/proliferation of SCAPs in the low level laser, red LED, and control groups at 24 or 48 h (P < 0.001). The ARS assessment showed that low level laser and red LED irradiation enhanced osteogenic differentiation of SCAPs. low level laser and red LED irradiation both induced over-expression of osteogenic/dentinogenic genes including alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) in SCAPs. Up-regulation of genes was significantly greater in low level laser irradiation group than red LED group (P < 0.001). Conclusion: Diode low level laser irradiation with 4 J/cm² energy density and red LED irradiation enhanced osteogenic differentiation of SCAPs without adversely affecting cell viability.