Amèlia Jansen van Vuuren
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Amèlia Jansen van Vuuren has a total of 11 co-authors. Here are the top co-authors by number of shared publications:
- Charlot Vandevoorde
GSI Helmholtzzentrum für Schwerionenforschung, Germany
1 shared publication - Farzana Rahiman
Skin Research Lab, Department of Medical Biosciences, University of the Western Cape
1 shared publication - Julie Bolcaen
Radiation Biophysics Division, SSC Laboratory, iThemba LABS, Cape Town 7131, South Africa
1 shared publication - Marco Durante
Biophysics Department, GSI Helmholtzzentrum für Schwerionenforschung, Planckstraße 1, 64291, Darmstadt, Germany; Institute of Condensed Matter Physics, Technische Universität Darmstadt, Hochschulstr. 6, 64289, Darmstadt, Germany; Department of Physics "Ettore Pancini", University Federico II, Via Cintia, 21 - Building 6, 80126, Naples, Italy
1 shared publication - Maryna de Kock
Department of Medical Bioscience, University of the Western Cape, Cape Town 7535, South Africa
1 shared publication - Monique Engelbrecht
Department of Medical Bioscience, Faculty of Natural Sciences, University of the Western Cape, Cape Town 7535, South Africa
1 shared publication
Latest Publications
- Open Access
Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (Loxodonta africana)
Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.
Open AccessEstablishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (Loxodonta africana)
Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.