Ao Tang

Rice yield and quality decline due to excessive fertiliser use is problematic in China. To increase rice grain filling and improve rice yield and quality, a nitrogen reduction and density increase study in 2023 and 2024 was imposed on a long-term experimental field. The four treatments adopted for the study were normal nitrogen and normal density (CK), normal nitrogen and increased density (NN+ID), reduced nitrogen in panicle fertiliser and increased density (RPN+ID), and reduced nitrogen in basal fertiliser and increased density (RBN+ID). RPN+ID and RBN+ID, respectively, produced a 3.0% and 5.1% higher yield than CK in both years. The mean grain filling rate (Va) of superior grains in RBN+ID increased by 12.5%, while the mean grain filling rate (Va) of inferior grains in the RPN+ID treatment increased by 4.2% with respect to CK. RPN+ID caused 0.4%, 9.6%, and 13.3% decline in the brown rice rate, chalkiness degree, and chalkiness rate, respectively, while RBN+ID triggered 0.4%, 7.2%, and 11.0% decline in the brown rice rate, chalkiness degree, and chalkiness rate, respectively. RPN+ID stimulated 4.2% and 3.1% increases in flavour and straight-chain amylose values, respectively. Whereas a 20% reduction in basal nitrogen fertiliser and a 32% increase in density improved the yield and appearance quality of rice, a 20% reduction in nitrogen fertiliser at the panicle stage and a 32% increase in density promoted a higher steaming flavour quality. Therefore, an appropriate reduction in nitrogen fertiliser while simultaneously increasing rice density has a significant impact on rice quality, fertiliser pollution reduction, and is a theoretical basis for rice yield and quality improvement in Northeast China.

Crop production is heavily dependent on fertilizers that negatively impact the environment; therefore, research on biochar to improve the soil’s properties and reduce greenhouse gas emissions has intensified over the years. To elucidate rice yield and greenhouse gas emission (GHG) arising from the application of biochar and N fertilizer on paddy soil in Northeast China, a 3-year (2015–2017) field experiment was established. Adopting a split-plot design with three replicates, two nitrogen (N) fertilizer levels in the main plots were designated as follows: 120 kg N ha⁻¹ (N1, 2/3 of N application rate for optimal local rice yield); 180 kg N ha⁻¹ (N2, full N application rate for optimal local rice yield); and four biochar application rates of no biochar (C0, control); 1.0 t ha⁻¹ biochar (C1); 1.5 t ha⁻¹ biochar (C2); and 2.0 t ha⁻¹ biochar (C3) were designated as sub-treatments. The results showed that in 2015, biochar amendment increased GHG emissions while between 2016 and 2017, biochar amendment of 1.5 t ha⁻¹ decreased CH₄ emissions, global warming potential (GWP), and greenhouse gasses intensity (GHGI) by 11.3%, 10.9%, and 17.0%, respectively. On average, for the years 2016 and 2017, the N₂O fluxes were 17.0% lower in the N2 plots compared to the N1 plots. Biochar amendment of 1.5 t ha⁻¹ recorded an 8.6% increase in rice yield compared to the control. The soil properties of the study site showed that biochar amendment of 1, 1.5, and 2 t ha⁻¹ augmented soil organic matter by 3.3%, 5.3%, and 5.2%, respectively, and soil phosphorus availability by 6.4%, 11.2%, and 22.6%, respectively. The co-application of biochar at 1.5 t ha⁻¹ and 180 kg N ha⁻¹ effectively regulated GHG emissions while maintaining crop yield. Appropriate co-application of biochar with N fertilizer can be adopted for emission reduction and rice yield maintenance while maintaining soil fertility in Northeast China.

Tillage practices are of critical importance in maintaining soil quality on cropland and for food production, with rice cultivation representing a significant portion of the world’s food production and greenhouse gas (GHG) emissions. While numerous studies have examined the effects of reduced and no-tillage on soil GHG emissions and rice yields, the impact of adopting a rotational approach to tillage practices on the rice cultivation cycle remains uncertain. In this study, we conducted a four-year (2017–2020) field experiment in a single rice-growing area in Northeast China with the aim of investigating the effects of different tillage practices on GHG emissions from paddy fields and rice yields under full straw return conditions. We set up three experimental treatments: rotary tillage, plowing, and rotational tillage (i.e., a combination of one year of plowing and one year of rotary tillage). The results showed that averaged across all treatments, average methane (CH₄, 302.6 ± 51.1 kg ha⁻¹) and nitrous oxide (N₂O, 0.86 ± 0.361 kg ha⁻¹) emissions and rice yield (9.0 ± 0.9 t ha⁻¹) did not exhibit significant inter-annual variability during the entire experimental period and were comparable to the average for the region. The ranking of GHG emissions during the rice-growing season was as follows: rotary tillage > plowing > rotational tillage. Across the experimental period, CH₄ and N₂O emissions were 9.1% and 8.5% lower in the plowing treatment and 21.2% and 13.1% lower in the rotational tillage treatment compared to the rotary tillage treatment. During the experimental period, there was no significant effect of tillage treatments on rice yield. This reduction in emissions may be attributed to changes in soil penetration resistance. In the rotational and plowing treatments, soil penetration resistance was in a range more adapted to rice growth and GHG emissions reduction compared to the rotary tillage treatment. The yield-scale GHG emission intensity was reduced by 12.7% and 26.1% in the plowing and rotational tillage treatments, respectively, in comparison to the rotary tillage treatment. This suggests that rotational tillage is a management practice that can achieve greenhouse gas emission reductions in paddy fields and stabilize or possibly increase rice yields. Consequently, the results demonstrated that a rotational alternation of multiple tillage practices is a synergistic strategy for achieving low carbon and high yield in rice in the cold rice-growing region of Northeast China.

The genetic diversity in 370 aromatic rice germplasms introduced from home and abroad, was investigated by using a total of 60 SSR markers. 361 alleles were detected, with the number of alleles (Na) per loci ranging from 2 to 10. The Nei's genetic diversity index (He) among loci varied widely from 0.104 at RM308 to 0.885 at RM2634 with an average value of 0.663. There was an obvious difference in SSR allelic diversity between indica and japonica rice. Indica rice showed more variation than japonica rice both for Na and He. Meanwhile, the genetic diversity of the landraces was higher than that of the improved varieties, and Na of the improved varieties was 86.5% of the landraces. Analysis of molecular variance indicated that 43.08% of the variation was from genetic differences between subspecies. It was also demonstrated that the genetic differentiation among different rice ecological regions ranged from 1.69% to 14.40%. There was significant differentiation between Southern China and Southwestern China, Central China and Southwestern China, respectively. Cluster analysis showed that 370 aromatic rice varieties were mainly classified into indica and japonica, and aromatic rice varieties from the same areas or the neighboring provinces were basically assigned to the same group.