Elodie Petit
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Elodie Petit has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Acácia Ferreira Vicente
ANSES, National and EU Reference Laboratory for Brucellosis , France
1 shared publication - Bruno Garin-Bastuji
French Agency for Food, Occupational and Environmental Health Safety (ANSES), EU/OIE/FAO Reference, Laboratory for Brucellosis, Maisons-Alfort 94706, France
1 shared publication - Claire Ponsart
ANSES, National and EU Reference Laboratory for Brucellosis , France
1 shared publication - Isabelle Jacques
Institut Universitaire Technologique (IUT), Département Génie Biologique, Université de Tours, 37082 Tours, France
1 shared publication - Luca Freddi
ANSES, National and EU Reference Laboratory for Brucellosis , France
1 shared publication - Maryne Jay
EU/OIE/FAO & National Reference Laboratory for animal Brucellosis, Animal Health Laboratory, Paris-Est University/Anses, Maisons-Alfort, France
1 shared publication
Latest Publications
- Open Access
- 31 July 2023
- Published by MDPI AG in Microorganisms
- Vol. 11 (8) , 1976
Evaluation of a Lateral Flow Immunochromatography Assay (LFIA) for Diagnosis and Surveillance of Brucellosis in French Alpine Ibex (Capra ibex)
France has been officially free of bovine brucellosis since 2005. Nevertheless, in 2012, as the source of two human cases, a bovine outbreak due to B. melitensis biovar 3 was confirmed in the French Alpine Bargy massif, due to a spillover from wild, protected Alpine ibex (Capra ibex). In order to reduce high Brucella prevalence in the local ibex population, successive management strategies have been implemented. Lateral flow immunochromatography assay (LFIA) was thus identified as a promising on-site screening test, allowing for a rapid diagnosis far from the laboratory. This study compared a commercial LFIA for brucellosis diagnosis with the WOAH-recommended tests for small ruminants (i.e., Rose Bengal test (RBT), Complement fixation test, (CFT) and Indirect ELISA, (iELISA)). LFIA showed the same analytical sensitivity as iELISA on successive dilutions of the International Standard anti-Brucella melitensis Serum (ISaBmS) and the EU Goat Brucella Standard Serum (EUGBSS). Selectivity was estimated at 100% when vaccinated ibex sera were analyzed. When used on samples from naturally infected ibex, LFIA showed high concordance, as well as relative sensitivity and specificity (>97.25%) in comparison with RBT and CFT. This work shows high reliability and ensures a better standardization of LFIA testing for wild ruminants.
- 31 July 2023
- Published by MDPI AG in Microorganisms
- Vol. 11 (8) , 1976
Open AccessEvaluation of a Lateral Flow Immunochromatography Assay (LFIA) for Diagnosis and Surveillance of Brucellosis in French Alpine Ibex (Capra ibex)
France has been officially free of bovine brucellosis since 2005. Nevertheless, in 2012, as the source of two human cases, a bovine outbreak due to B. melitensis biovar 3 was confirmed in the French Alpine Bargy massif, due to a spillover from wild, protected Alpine ibex (Capra ibex). In order to reduce high Brucella prevalence in the local ibex population, successive management strategies have been implemented. Lateral flow immunochromatography assay (LFIA) was thus identified as a promising on-site screening test, allowing for a rapid diagnosis far from the laboratory. This study compared a commercial LFIA for brucellosis diagnosis with the WOAH-recommended tests for small ruminants (i.e., Rose Bengal test (RBT), Complement fixation test, (CFT) and Indirect ELISA, (iELISA)). LFIA showed the same analytical sensitivity as iELISA on successive dilutions of the International Standard anti-Brucella melitensis Serum (ISaBmS) and the EU Goat Brucella Standard Serum (EUGBSS). Selectivity was estimated at 100% when vaccinated ibex sera were analyzed. When used on samples from naturally infected ibex, LFIA showed high concordance, as well as relative sensitivity and specificity (>97.25%) in comparison with RBT and CFT. This work shows high reliability and ensures a better standardization of LFIA testing for wild ruminants.